Evaluation the safety and synergistic effect of NiFe2O4 nanoparticles with antibiotic against Pseudomonas aeruginosa

Abstract

The antimicrobial resistance currently impedes and threatens the future of effective prevention and treatment of the continually expanding range of infections caused by bacteria. This study aimed to identify the bacterial causes the wound infection among animals and using the antibiotic/nanoparticles mixture as a new attempt for the treatment the wound infection induced in rats. For this purpose, 112 swabs wound infection cases in the different animal types (36 sheep, 21 goats, 12 cows, 4 horses, 8 dogs, 9 rabbits, 7 genies pigs and 15 rats) were studied in the for bacterial isolation. The Pseudomonas aeruginosa was tested for its sensitivity to the antibiotics and the nanoparticles (CoFe2O4 and NiFe2O4) in vitro by using the MIC method. Also the wound infection was induced in the rats and the effect of nanoparticles/antibiotics mixture were tested in vivo. The results showed that P. aeruginosa was the predominant bacterial type that the caused wound infection. The minimum inhibitor concentration of NiFe2O4 and CoFe2O4 nanoparticles were 32 µg /ml and 16 µg /ml respectively. A clear synergistic effect of antibiotic/ nanoparticles as antibacterial were noticed which appear as a decrease in MIC and increase of the inhibitory diameter zone. According to the result of Random Amplification of Polymorphic DNA test, the nanoparticles effects on genetic material of P. aeruginosa observed as an appearance/disappearance of bands, increase in thickness and clarity of the bands.

Effect of garlic oil nanoemulsion against multidrug resistant Pseudomonas aeruginosa isolated from broiler

“…The MIC method was used to assess the sensitivity of Pseudomonas aeruginosa to the antibiotics and the nanoparticles (CoFe2O4 and NiFe2O4) in vitro which had minimum inhibitor concentrations of 32 g/ml and 16 g/ml, respectively. The appearance/disappearance of bands, an increase in the thickness and clarity of the bands, and other effects of nanoparticles on P. aeruginosa's genetic material were noted in the results of the Random Amplification of Polymorphic DNA test (62).…”

The Antibacterial Activity of Biosynthesized Silver Nanoparticles from Tribulus terrestris Against Pseudomonas aeruginosa

“…The agar was excavated using a sterile 5 mm cork borer, and 100 μl of Silver nanoparticles at each concentration (25, 50, 100 and 150 µg/ml) were added to each well. After incubating Petri plates at 37°C for 24 hours, the inhibitory zone width was measured in millimeters using a zone reader [16].…”

Synthesis and Characterization of Tetracycline Loaded Methionine-Coated NiFe2O4 Nanoparticles for Anticancer and Antibacterial Applications

“…Different concentrations of Tet-Met-NiFe 2 O 4 (0-70 µg/mL) were added to the cultured A375 and HFF cells and incubated for 72 h. For cell proliferation studies, the cells were incubated with 0.5 mg/mL of MTT reagent for 4 h. Then, the purple crystal formazan formed was dissolved in 100 µL of DMSO for the colorimetric determination of the cells' oxidoreductase enzymatic activity. The absorbance values of both control and test concentrations were measured at 570 nm while reference blank measurements taken at 630 nm, and then the cell viability and IC 50 values were calculated [7,15,37]. The percentage of cell viability was calculated using Equation (1).…”