Evaluation the Surface Antigen of theSalmonella typhimuriumATCC 14028 Ghosts Prepared by “SLRP”

Abstract

Recently, bacterial ghosts (BGs) were prepared using a protocol based on critical chemical concentrations. It has been given the name “sponge like” (SL) protocol and used in its reduced form “sponge like reduced protocol” (SLRP). While specific antibody for Salmonella is available on the market under the commercial names (of some kits) such as Febrile Antigen Kit (N.S. BIO-TEC), we used the described Kit to investigate the validity of the SLRP. In this study, using SLRP we succeeded to prepare STGs with correct surface antigens could interact with their specific antibodies. Additionally the study has included oral vaccination with STGs with challenge test. The rats serums have been evaluated against both of the O and H antigens. The antigen-antibody interaction (agglutination) results of both the SLRP and the animal experiments prove that we have correct STGs able to immunize the rats against viable Salmonella. STGs could be used as vaccine and as adjuvant and in the antibodies and in the diagnostic kits production. This study is an additional step for the establishment of correct BGs for immunological purposes.

Cracking the Microbial Cell Wall

“…The protocol describes a new method for bacterial evacuation from their cytoplasmic contents without deforming their 3D structure or their surface antigens [1,2]. The protocol was originally optimized by Plackett-Burman design [1,3].…”

“…The used chemical compounds are SDS, NaOH, H 2 O 2 , CaCO 3 and the NaCl and the Ethanol were used during the evacuation processes. By determining each of the MIC and MGC and running either the full Plackett-Burman twelve experimental design as in the original protocol or running the best two experiments which giving the best results as in the reduced protocol, that guarantee full evacuation with nearly no effect on the 3D or the surface antigens [4,2].…”

Lysozymes, Proteinase K, Bacteriophage E Lysis Proteins, and some Chemical Compounds for Microbial Ghosts Preparation: a Review and Food for Thought

“…BGs can be used in the diagnostic Kit as a reference antigen, where it should give positive reaction with serum containing the proper antibodies [34].…”

Protein and DNA Isolation from Aspergillus Niger as well as Ghost Cells Formation

“…Its main concept is using active chemical compounds that could introduce pores in the microbes and degrade the DNA at concentrations which did not change the surface antigens or the 3D structure [13]. This enables evacuating gram-negative and gram-positive bacteria, eukaryotes and viruses [4,[12][13][14][16][17][18][19][20][21][22][23][24][25][26][27].…”