Evalution of “indirect fluorescent antibody test‟ as potential screening test for Mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock

Abstract

In the present study, Indirect Fluorescent antibody test (iFAT) has been used as the "screening test" for the detection of MAP bacilli in the milk samples of lactating domestic livestock. A total of 372 milk samples from lactating animals were screened by iFAT and results were compared with microscopy of milk samples and milk ELISA test. Comparative analysis of the results of three tests showed that iFAT had fairly good sensitivity (84.7%) and specificity (90.4%), with respect to ZN staining (microscopy) with kappa value of 0.735 and "good" strength of agreement. Similar comparison with milk ELISA test revealed, sensitivity (73.3%) and specificity of (72.6%) with kappa value of 0.443 and strength of agreement was moderate. Lower or higher difference in sensitivity and specificity of iFAT with respect to ZN staining and milk ELISA may be due to the difference in detection "target" of the test i.e., antigen or antibody. It was concluded that iFAT was a reliable and sensitive diagnostic test for the detection of MAP in milk and can also be used for the "mass screening" of the milk samples.

Serodiagnosis of map infection by latex agglutination test in slaugtered buffaloes of Malwa region (M.P., India)

“…The results of screening of anti-MAP antibodies by latex agglutination test are shown in Table1, Figure1, 2 and 3. The present investigation was in consonance with the findings ofSingh et al (2016) who observed 51.4% infection in bovine milk samples and with 50.9% infection in paneer samples(Stephen et al, 2016).…”

BIO-INCIDENCE OF Mycobacterium avium SUBSPECIES paratuberculosis IN THE PASTEURIZED LIQUID MILK, FLAVOURED MILK AND MILK POWDER COMMERCIALLY SOLD BY LEADING MARKET BRANDS IN INDIA

“…Test was performed as per Singh et al (2016a). Briefly, smears were prepared on clean slides from (20 µl commercial milk) samples, air dried at room temperature and heat fixed.…”

“…screening of milk and milk products and to estimate bio-incidence of MAP Singh et al, 2016a;Singh et al, 2016b;Singh et al, 2016c). Present study first time used both traditional and newly developed and standardized serological and molecular tests (microscopy, IS900 PCR, i_ELISA, d_ELISA, LAT and i_FAT) to estimate bio-incidence of MAP in liquid pasteurized milk and milk products (flavoured milk and milk powder) made from pasteurized milk and sold by 10 leading commercial market brands in Mathura and Agra districts of South UP in North India.…”

Bio-detection of Mycobacterium avium subspecies paratuberculosis in the Commercial Milk and Milk Products Sold in Bhubaneswar, Odisha, India

“…Test was performed as per Singh et al, (2016a). Briefly, smears were prepared on clean slides from (20 µl commercial milk and milk products), air dried and heat fixed.…”