Evasion of host defense by Brucella

Yang, Jinke; Wang, Yue; Hou, Yuanpan; Sun, Mengyao; Xia, Tian; Wu, Xin

doi:10.1016/j.cellin.2023.100143

Cited by 4 publications

(2 citation statements)

References 143 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The anticipated outcome of the conducted in vivo studies is the induction of a Th1 response in the mouse immune system [52], reveals by elevated concentrations of IFN-γ, TNF-α, and IL-12 from the evaluated cytokines. Cytokine levels of IFNγ, TNF-α, IL-12 and IL-4 are shown in figure 4.…”

Section: Cytokine Production Assaymentioning

confidence: 99%

Construction of recombinant Omp25 or EipB protein loaded PLGA nanovaccines for Brucellosis protection

Akmayan,

Oztav,

Coksu

et al. 2024

Nanotechnology

View full text Add to dashboard Cite

Safe and effective vaccine candidates are needed to address the limitations of existing vaccines against Brucellosis, a disease responsible for substantial economic losses in livestock. The present study aimed to encapsulate Omp25 and EipB proteins, knowledged antigen properties, into PLGA nanoparticles, characterize synthesized nanoparticles with different methods, and assessed their in vitro/in vivo immunostimulatory activities to develop new vaccine candidates. The rOmp25 and EipB proteins produced with recombinant DNA technology were encapsulated into PLGA nanoparticles by double emulsion solvent evaporation technique. The nanoparticles were characterized using SEM, Zeta-sizer, and FTIR instruments to determine size, morphology, zeta potentials, and polydispersity index values, as well as to analyze functional groups chemically. Additionally, the release profiles and encapsulation efficiencies were assessed using UV–Vis spectroscopy. After loading with recombinant proteins, O-NPs reached sizes of 221.2±5.21 nm, while E-NPs reached sizes of 274.4±9.51 nm. The cumulative release rates of the antigens, monitored until the end of day 14, were determined to be 90.39% for O-NPs and 56.1% for E-NPs. Following the assessment of the in vitro cytotoxicity and immunostimulatory effects of both proteins and nanoparticles on the J774 murine macrophage cells, in vivo immunization experiments were conducted using concentrations of 16 µg/ml for each protein. Both free antigens and antigen-containing nanoparticles excessively induced humoral immunity by increasing produced Brucella-specific IgG antibody levels for 3 times in contrast to control. Furthermore, it was also demonstrated that vaccine candidates stimulated Th1-mediated cellular immunity as well since they significantly raised IFN-gamma and IL-12 cytokine levels in murine splenocytes rather than IL-4 following to immunization. Additionally, the vaccine candidates conferred higher than 90% protection from the infection according to challenge results. Our findings reveal that PLGA nanoparticles constructed with the encapsulation of recombinant Omp25 or EipB proteins possess great potential to trigger Brucella-specific humoral and cellular immune response.

show abstract

Section: Cytokine Production Assaymentioning

confidence: 99%

Construction of recombinant Omp25 or EipB protein loaded PLGA nanovaccines for Brucellosis protection

Akmayan,

Oztav,

Coksu

et al. 2024

Nanotechnology

View full text Add to dashboard Cite

show abstract

“…Autophagy is one of the main elimination mechanisms to degrade intracellular pathogens. It involves the coordinated actions of immune cells like macrophages, immune molecules like pattern recognition receptors (PRRs), pathogen-associated molecular patterns (PAMPs), and complement systems ( Yang et al., 2024 ). Although almost 90% of internalized Brucellae are killed with the first stage of infection, some evade the cell-mediated immunity by stealthy mechanisms which enable them survive along with the immune responses and replicate within intracellular niches ( Amjadi et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Role of myeloid-derived suppressor cells in chronic brucellosis

Hou,

Kong,

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

IntroductionHuman brucellosis, a Brucella infection caused most common zoonosis in the world, remains a serious public health burden in China. Brucella chronic infection always causes immunosuppressive status and results in severe organ or tissue damages. The aim of this work was to study the role of the myeloid-derived suppressor cells (MDSCs) in human chronic brucellosis.MethodsFifty cases of chronic brucellosis and 40 healthy individual controls were enrolled in this study. We analyzed the frequency and subsets of MDSCs in PBMC between the chronic brucellosis and healthy control groups by flow cytometry. Furthermore, we also measured the inflammatory-related cytokines in serum samples and the MDSCs inhibition ability to the proliferation of T cells in vitro.ResultsWe found that the frequency of MDSCs in peripheral blood and the level of IL-6 and IL-10 Th2 cytokines and Arginase-1 were significantly increased in chronic brucellosis patients. In addition, we also found that the T cell function was suppressed in vitro by co-culturing with MDSCs from brucellosis patients.ConclusionOur study described an increase of immunosuppressive MDSCs in peripheral blood of chronic brucellosis patients. These results contribute to the understanding of Brucella persistent infection, which may provide an insight for effective treatment of chronic brucellosis patients in clinical practice.

show abstract

SIRT2 Inhibition Enhances Mitochondrial Apoptosis in Brucella-Infected Bovine Placental Trophoblast Cells

Zhang,

Qi,

et al. 2024

Preprint

View full text Add to dashboard Cite

Brucella being a successful pathogen, employs a plethora of immune evasion mechanisms. This contributes to pathogenesis, persistence and also limits the efficacy of available treatment. Increasing understanding of host-pathogen interactions suggests that integrating host-directed strategies with existing anti-Brucella treatments could lead to more effective bacterial clearance and a reduction in drug-resistant strains. SIRT2 is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase found in mammals. It can deacetylate various transcription factors and regulatory proteins, playing a crucial role in host-pathogen interactions and pathogen infection-induced apoptosis. In this study, we investigate the role of SIRT2 in Brucella-induced cell apoptosis using bovine placental trophoblast cells. Our results indicate that B. abortus A19 infection upregulates SIRT2 protein expression and significantly induces mitochondrial apoptosis in these cells. Furthermore, Inhibition of SIRT2 exacerbates B. abortus A19-induced mitochondrial apoptosis and markedly inhibits intracellular bacterial survival. These results prove the role of SIRT2 in Brucella pathogenesis and the mechanism of action.

show abstract

Evasion of host defense by Brucella

Cited by 4 publications

References 143 publications

Construction of recombinant Omp25 or EipB protein loaded PLGA nanovaccines for Brucellosis protection

Construction of recombinant Omp25 or EipB protein loaded PLGA nanovaccines for Brucellosis protection

Role of myeloid-derived suppressor cells in chronic brucellosis

SIRT2 Inhibition Enhances Mitochondrial Apoptosis in Brucella-Infected Bovine Placental Trophoblast Cells

Contact Info

Product

Resources

About