Evasion of mucosal defenses during Aeromonas hydrophila infection of channel catfish (Ictalurus punctatus) skin

Li, Chao; Wang, Ruijia; Su, Baofeng; Luo, Yongchao; Terhune, Jeffery S.; Beck, Benjamin H.; Peatman, Eric

doi:10.1016/j.dci.2012.11.009

Cited by 100 publications

(85 citation statements)

References 76 publications

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“…For instance, the expression of alkaline phosphatase in the zebrafish intestinal epithelium in response to the gut microbiota can dephosphorylate and thus detoxify lipopolysaccharide, a structural and immunodominant molecule present on the outer membranes of Gram-negative bacteria (Bates et al, 2007). In contrast, it is widely recognized that fish can develop severe infections in the presence of wounds (Chu and Lu, 2008) or under stressful growth conditions, such as an unfavorable water temperature, poor water quality, or high stocking density (Li et al, 2013). Therefore, fish reared under intensive conditions are susceptible to infection by opportunistic A. hydrophila because of their high stress levels.…”

Section: Discussionmentioning

confidence: 95%

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Aeromonas hydrophila induces intestinal inflammation in grass carp (Ctenopharyngodon idella): An experimental model

et al. 2014

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Section: Discussionmentioning

confidence: 95%

“…in a variety of farmed fish, including the rainbow trout and grass carp (Nya and Austin, 2009;Xu et al, 1987). As an opportunistic pathogen, A. hydrophila typically does not cause disease in fish unless the fish are stressed or unhealthy (Li et al, 2013). This is largely because the host can maintain a homeostasis and tolerate the pathogen.…”

Section: Discussionmentioning

confidence: 98%

Aeromonas hydrophila induces intestinal inflammation in grass carp (Ctenopharyngodon idella): An experimental model

et al. 2014

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“…MBLs have been identified in the proteome of skin mucus from Atlantic cod, indicating they may be present constitutively at barrier defenses. 71 In catfish, we have observed induction of MASP2 in catfish gill following F. columnare infection, 47 higher levels of MBL1 in channel and blue catfish skin following A. hydrophila infection, 38,39 and 86-fold higher expression of MBL2b in resistant channel catfish gill tissue compared to susceptible gill at 1 h following F. columnare challenge. 25 Galectins constitute a lectin family defined by their binding specificity for b-galactoside sugars.…”

Section: Lectinsmentioning

confidence: 95%

“…Microarray analyses revealed differential expression patterns of MUC5AC, MUC5B at early timepoints following challenge in the more susceptible channel catfish but not in blue catfish. 38,39 The gaps in our understanding of mucin dynamics in catfish skin are numerous. Mucin secretion in the skin, like the gill, undoubtedly responds to complex signals from environment, host, pathogen, and the commensal microbiome.…”

Section: Mucins In the Gillmentioning

confidence: 99%

“…44 Relatedly, after infection with Aeromonas hydrophila, several components of desmosomes including desmocollin 1a, desmoglein and plakoglobin were perturbed in the skin of channel catfish. 38 Transcriptomic profiling of gill and skin after an experimental exposure to Flavobacterium columnare showed rapid (by 1 hr) and robust alterations in the expression of factors related to junctional processes. 25 Intriguingly, a comparison of na€ ıve fish obtained from columnaris resistant and susceptible families revealed that some claudin family members in resistant fish were expressed at levels >100 fold lower than resistant fish.…”

Section: Cell Junctionsmentioning

confidence: 99%

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Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

et al. 2015

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The mucosal barriers of catfish (Ictalurus spp) constitute the first line of defense against pathogen invasion while simultaneously carrying out a diverse array of other critical physiological processes, includ\ing nutrient adsorption, osmoregulation, waste excretion, and environmental sensing. Catfish depend more heavily on mucosal barriers than their terrestrial counterparts as they are continuously interacting with the aquatic microbiota. Our understanding of these barriers, while growing, is still limited relative to that of mammalian model systems. Nevertheless, a combination of molecular and cellular studies in catfish over the last few decades, and particularly within the last few years, has helped to elucidate many of the primary actors and pathways critical to their mucosal health. Here we describe aspects of innate and adaptive immune responses in the primary mucosal tissues (skin, gill, and intestine) of catfish, focusing on mucus-driven responses, pathogen recognition, soluble mediators, and immunoglobulin and T-cell derived immunity. Modulation of mucosal barriers will be critical moving forward for crafting better diets, improving vaccine delivery, enhancing water quality, and ensuring sustainable production practices in catfish.

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Effectiveness of Cathelicidin Antimicrobial Peptide against Ictalurid Catfish Bacterial Pathogens

et al. 2021

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One of the major goals in aquaculture is to protect fish against infectious diseases as disease outbreaks could lead to economic losses if not controlled. Antimicrobial peptides (AMPs), a class of highly conserved peptides known to possess direct antimicrobial activities against invading pathogens, were evaluated for their ability to protect Channel Catfish Ictalurus punctatus and hybrid catfish (female Channel Catfish × male Blue Catfish I. furcatus) against infection caused by the fish pathogen Aeromonas hydrophila ML09‐119. To identify effective peptides, the minimum inhibitory concentrations against bacterial pathogens Edwardsiella ictaluri S97‐773, Edwardsiella piscicida E22‐10, A. hydrophila ML09‐119, Aeromonas veronii 03X03876, and Flavobacterium columnare GL‐001 were determined in vitro. In general and overall, cathelicidins derived from alligator and sea snake exhibited more potent and rapid antimicrobial activities against the tested catfish pathogens as compared to cecropin and pleurocidin AMPs and ampicillin, the antibiotic control. When the peptides (2.5 µg of peptide/g of fish) were injected into fish and simultaneously challenged with A. hydrophila through immersion, increased survival rates in Channel Catfish and hybrid catfish were observed in both cathelicidin (alligator and sea snake) treatments as compared to other peptides and the infected control (P < 0.001) with alligator cathelicidin being the overall best treatment. Bacterial numbers in the kidney and liver of Channel Catfish and hybrid catfish also decreased (P < 0.05) for cathelicidin‐injected groups at 24 and 48 h after challenge infection. These results show the potential of cathelicidin to protect catfish against bacterial infections and suggest that an approach overexpressing the peptide in transgenic fish, which is the long‐term goal of this research program, may provide a method of decreasing bacterial disease problems in catfish as delivering the peptides via individual injection or feeding would not be economically feasible.

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Evasion of mucosal defenses during Aeromonas hydrophila infection of channel catfish (Ictalurus punctatus) skin

Cited by 100 publications

References 76 publications

Aeromonas hydrophila induces intestinal inflammation in grass carp (Ctenopharyngodon idella): An experimental model

Aeromonas hydrophila induces intestinal inflammation in grass carp (Ctenopharyngodon idella): An experimental model

Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

Effectiveness of Cathelicidin Antimicrobial Peptide against Ictalurid Catfish Bacterial Pathogens

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