2000
DOI: 10.1002/(sici)1097-0215(20000215)85:4<503::aid-ijc10>3.0.co;2-l
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Evidence against a role of general protein kinase C downregulation in skin tumor promotion

Abstract: Using isoenzyme‐specific antibodies, we have performed an immunoblot analysis of the PKC isoenzyme pattern during the course of TPA‐induced tumor promotion in the epidermis of NMRI mice. The TPA‐sensitive PKC isoforms α, δ, ϵ, η, μ (and TPA‐insensitive PKCζ), but not PKCβ and γ, were found to be expressed in both normal and neoplastic epidermis. The immune signals of all TPA‐sensitive PKC isoforms were moderately and reversibly attenuated upon a single TPA treatment. Using different antibodies against PKCη and… Show more

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Cited by 11 publications
(3 citation statements)
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“…Our results partially support this hypothesis. However, there is evidence against the association between PKC down-regulation and skin tumor promotion (61).…”
Section: Discussionmentioning
confidence: 99%
“…Our results partially support this hypothesis. However, there is evidence against the association between PKC down-regulation and skin tumor promotion (61).…”
Section: Discussionmentioning
confidence: 99%
“…UVB light induces overexpression of ODC an enzyme that plays a critical role in photocarcinogenesis (Jansen et al, 2001). Therefore, the ODC activity served as a marker of the mutagenic and carcinogenic effects of UV light, because the activity of ODC in quiescent cells is extremely low and readily induced by UV light (Manzow et al, 2000). Furthermore, ODC is also activated by many other stimuli including tumor promoters and growth factors (Oguro and Yoshida, 2001), as well.…”
Section: Introductionmentioning
confidence: 99%
“…Phorbol ester-mediated downregulation of PKC protein levels in the mouse skin promotion model has been documented 73,74 . That phorbol esters induced acute activation of PKCs, followed by chronic downregulation of PKC protein levels, begs the question of whether cPKC and nPKCs in this context function as oncogenic drivers and/or as tumour suppressors.…”
Section: [H2] Phorbol Ester-mediated Tumour Promotionmentioning
confidence: 99%