Evidence and Consequence of Porcine Endogenous Retrovirus Recombination

Bartosch, Birke; Stefanidis, Dimitrios; Myers, Richard; Weiss, Robin A.; Patience, Clive; Takeuchi, Yasuhiro

doi:10.1128/jvi.78.24.13880-13890.2004

Cited by 111 publications

(91 citation statements)

References 51 publications

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“…Alternatively, these distinct bursts may be explained by the presence of active elements in the genome only during a short period (corresponding to the peaks). These active elements may have arisen from ectopic recombinations between two defective copies, a mechanism known for retroviruses (Bartosch et al 2004). The presence of RIRE1, Kangourou, and Wallabi in the genome of many other Oryza species (Fig.…”

Section: Genomic Expansion In Oryzamentioning

confidence: 99%

Doubling genome size without polyploidization: Dynamics of retrotransposition-driven genomic expansions in Oryza australiensis, a wild relative of rice

Piégu¹,

Guyot²,

Picault³

et al. 2006

Genome Res.

542

472

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Retrotransposons are the main components of eukaryotic genomes, representing up to 80% of some large plant genomes. These mobile elements transpose via a “copy and paste” mechanism, thus increasing their copy number while active. Their accumulation is now accepted as the main factor of genome size increase in higher eukaryotes, besides polyploidy. However, the dynamics of this process are poorly understood. In this study, we show that Oryza australiensis, a wild relative of the Asian cultivated rice O. sativa, has undergone recent bursts of three LTR-retrotransposon families. This genome has accumulated more than 90,000 retrotransposon copies during the last three million years, leading to a rapid twofold increase of its size. In addition, phenetic analyses of these retrotransposons clearly confirm that the genomic bursts occurred posterior to the radiation of the species. This provides direct evidence of retrotransposon-mediated variation of genome size within a plant genus.

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Section: Genomic Expansion In Oryzamentioning

confidence: 99%

Doubling genome size without polyploidization: Dynamics of retrotransposition-driven genomic expansions in Oryza australiensis, a wild relative of rice

Piégu¹,

Guyot²,

Picault³

et al. 2006

Genome Res.

542

472

View full text Add to dashboard Cite

show abstract

“…To determine the PERV viral load, RNA was exhuman cells in vitro (2,12,36). Using the coculture infectivity test with human HEK293 and swine St Iowa tracted from pig blood plasma, reverse transcribed, and cDNA tested by real-time PCR.…”

Section: Quantification Of Perv Copy Number In Dpf Pigsmentioning

confidence: 99%

Porcine Endogenous Retrovirus (PERV) and its Transmission Characteristics: A Study of the New Zealand Designated Pathogen-Free Herd

et al. 2008

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Previously a strategy for monitoring of pigs intended for cell transplantation was developed and successfully applied to several representative herds in New Zealand. A designated pathogen-free (DPF) herd has been chosen as a good candidate for xenotransplantation. This herd has previously tested free of infectious agents relevant to xenotransplantation and we present here an in depth study of porcine endogenous retrovirus (PERV) transmission. A panel of assays that describes the constraints for the transmission of PERV has been suggested. It includes a) infectivity test in coculture of DPF pig primary cells with both human and pig target cell lines; b) RT activity in supernatant of stimulated primary cells from DPF pigs; c) viral load in donor's blood plasma; d) PERV proviral copy number in DPF pig genome; e) PERV class C prevalence in the herd and its recombination potential.

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“…Los genes de la clase I y III se localizan en el brazo corto mientras que los genes clase II se localizan en el brazo largo del mismo cromosoma. La región de las moléculas Clase I contienen en su interior doce genes, de los cuales 4 son funcionales; los genes tipo II codifican para las moléculas Clase II y los genes clase III codifican para factores de complemento, situación que es similar en el humano (27,28).…”

Section: Cmh En Cerdosunclassified

“…Por tal motivo, se ha estudiado el desarrollo de zoonosis a causa de agentes patógenos específicos de los porcinos, como es el caso del retrovirus endógeno porcino (PERV), el cual se multiplica en las células mononucleares periféricas, cé-lulas endoteliales, los islotes pancreáticos y algunas líneas celulares del riñón del cerdo (28,29,44,45).…”

Section: Riesgos Del Xenotransplanteunclassified

Del alotransplante al xenotransplante: la compatibilidad antigénica donante-receptor por medio del Complejo Mayor de Histocompatibilidad CMH

Amaya

Navarrete

2006

nova

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ResumenLos transplantes de órganos han sido una herramienta del área clínica para la búsqueda del mejoramiento de la calidad en la salud humana e igualmente brinda al paciente una nueva oportunidad de vida. Esta solución se ha visto obstaculizada por el déficit de órganos suficientes ya que para su obtención se han encontrado grandes dificultades, dentro de las cuales se pueden nombrar la negativa de las familias, los costos, la demora en la captación del órgano, la falta de infraestructura logística que permita realizarlo en un tiempo óptimo, las fallas en el transporte del órgano poniendo en riesgo el éxito del transplante, entre otras. Por todo lo anterior y teniendo en cuenta el posible rechazo a causa de la compatibilidad de los complejos reconocedores de antígeno entre donante y receptor, se ha fijado la mirada en órganos de animales denominados xenogénicos que puedan suplir esta problemática y nos permita tener una alternativa fácil de obtención de órganos para las personas que así lo requieran. Esta publicación tiene como objeto realizar una revisión amplia de las temáticas relacionadas con el transplante, con el fin de analizar desde el punto de vista molecular e inmunológico las ventajas y desventajas de un transplante xenogénico.Palabras claves: alogénico, complejo mayor de histocompatibilidad (CMH), histocompatibilidad, linfocitos T, polimorfismo, xenotransplante. AbstractOrgan transplants have been a clinical area tool for the search of the improvement of the quality in the human health and it also offers the patient a new opportunity of life. This solution has been blocked by the deficit of enough organs. To obtain them there have been big difficulties like the negative of the families, the costs, the delay in the reception of the organ, the lack of logistical infrastructure that allows to carry it in a timely fashion, the flaws in the transport of the organ risking the success of the transplant, among others. For all the above and keeping in mind the possible rejection because of the compatibility of the complex antigen to recognize among donor and receiver, the scientists have looked at organs of animals denominated xenogenics that can solve this problem and it will allow us to have an alternative for the easy obtaining of organs for people that require it. This publication tries to carry out a wide revision of the contents of the transplant with the purpose of analyzing the advantages and disadvantages of a xenogenic transplant from the molecular and immunologic point of view. ARTÍCULO DE REVISIÓN

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Evidence and Consequence of Porcine Endogenous Retrovirus Recombination

Abstract: The genetic nature and biological effects of recombination between porcine endogenous retroviruses (PERV) were studied. An infectious molecular clone was generated from a high-titer, human-tropic PERV isolate,

Cited by 111 publications

References 51 publications

Doubling genome size without polyploidization: Dynamics of retrotransposition-driven genomic expansions in Oryza australiensis, a wild relative of rice

Doubling genome size without polyploidization: Dynamics of retrotransposition-driven genomic expansions in Oryza australiensis, a wild relative of rice

Porcine Endogenous Retrovirus (PERV) and its Transmission Characteristics: A Study of the New Zealand Designated Pathogen-Free Herd

Del alotransplante al xenotransplante: la compatibilidad antigénica donante-receptor por medio del Complejo Mayor de Histocompatibilidad CMH

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