Periprosthetic joint infection (PJI) remains a challenging complication and is a leading reason for failure following total joint arthroplasty (TJA). There is a significant economic burden to the treatment of PJI. [1] In addition to the economic burden, elderly patients with comorbid conditions are at increased risk of postoperative morbidity and mortality when undergoing treatment of PJI. [2] Currently, many different strategies are used to mitigate the risk of PJI after TJA and newer approaches are constantly in development. Several organizations have subsequently recommended evidence-based guidelines for the prevention of PJI and surgical site infections (SSIs). The most notable are the International Consensus Meeting (ICM), [3] World Health Organization (WHO), [4] and the Center for Disease Control and Prevention (CDC) [5] guidelines. In parallel to new scientific developments, these guidelines sometimes change their recommendations. However, the alignment of orthopedic surgeons with these guidelines may differ due to their experiences Objectives: This study aims to assess the methods employed by Turkish orthopedic surgeons to prevent periprosthetic joint infection (PJI) in total joint arthroplasty (TJA). Patients and methods: The data obtained for this study, conducted between January 2019 and February 2019, were gathered by sending out an online survey to Turkish Society of Orthopedics and Traumatology members (n=2,267). A total of 354 orthopedic surgeons responded and completed survey. The survey had 23 questions which include the experience, academic position, hospital where the physician works, monthly arthroplasty numbers, and infection prevention methods employed before, during, and after surgery. Results: The period for antibiotics prophylaxis showed variability, with about 63% of surgeons using prophylaxis longer than 24 hours. In terms of academic position, 52.4% of professors and 52.8% of associate professors used prophylaxis for the first 24 hours whereas this rate was 31.3% in operators (p=0.01). Of surgeons, 50.7% who perform more than 10 arthroplasties per month and 33.6% of surgeons who perform less than 11 arthroplasties per month used 24-hour antibiotic prophylaxis (p=0.006). Blood glucose level assessment prior to surgery was performed by the majority of surgeons (94%). A total of 118 orthopedic surgeons (33.3%) performed methicillinresistant Staphylococcus aureus (MRSA) decolonization with 54.7% of associate professors, 59.5% of professors, and 24.7% of operators (p=0.001). Only 60 surgeons (16.9%) checked vitamin D levels. Conclusion: Our study results demonstrated that the majority of orthopedic surgeons in Turkey do not follow the antibiotic prophylaxis recommendations and they use antibiotic prophylaxis for longer periods. Professors and associate professors, and surgeons with higher monthly arthroplasty numbers than surgeons with lower monthly arthroplasty numbers follow the recommended periods more than their counterparts. Most surgeons assess blood glucose levels whereas a small num...