Evidence for 5,12-dihydroxy-6,8,10,14-eicosatetraenoate as a mediator of human neutrophil aggregation

O’Flaherty, Joseph T.; Hammett, M.J.; Shewmake, T.B.; Wykle, Robert L.; Love, Samuel H.; McCall, Charles E.; Thomas, Michael J.

doi:10.1016/0006-291x(81)90487-3

Cited by 56 publications

(18 citation statements)

References 12 publications

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“…AGEPC desensitizes subsequent AGEPC-or LTB4-induced aggregation equally well, but LTB4 desensitizes subsequent LTB4-induced aggregation more readily than subsequent AGEPC-induced aggregation. These data agree with the experiments of O'Flaherty et al (20), who found essentially the same degree of crossover between the two agonists. However, our data do not agree with the data of FordHutchinson (19), who found no evidence of cross-desensitization between AGEPC and LTB4.…”

supporting

confidence: 92%

“…Platelet-activating factor (PAF)' is a potent chemical mediator released from antigen-stimulated, IgE-senReceived for publication 20 April 1982 and in revised form 20 July 1982.…”

Section: Introductionmentioning

confidence: 99%

“…LTB4 is also a potent inducer of leukocyte aggregation, degranulation, and chemotaxis (14)(15)(16)(17)(18). Using desensitization experiments, conflicting evidence has appeared concerning the relationship(s) between AGEPC and hydroxy acid derivatives of arachidonic acid (19,20 In experiments where LTB4 and 5S,12S diHETE were separated, the same column was used, but an isocratic system of acetonitrile:water:acetic acid (65:35:0.1; 1.0 ml/min) was used for elution. Detection was at 280 nm with both systems using a Tracor model 970A detector (Tracor Analytic, Elk Grove Village, IL).…”

Section: Introductionmentioning

confidence: 99%

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Acetyl glyceryl ether phosphorylcholine stimulates leukotriene B4 synthesis in human polymorphonuclear leukocytes.

Lin¹,

Morton²,

Gorman³

1982

J. Clin. Invest.

172

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supporting

confidence: 92%

“…Platelet-activating factor (PAF)' is a potent chemical mediator released from antigen-stimulated, IgE-senReceived for publication 20 April 1982 and in revised form 20 July 1982.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acetyl glyceryl ether phosphorylcholine stimulates leukotriene B4 synthesis in human polymorphonuclear leukocytes.

Lin¹,

Morton²,

Gorman³

1982

J. Clin. Invest.

172

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“…The results suggest that the receptor site which interacts with UTP and ATP on the neutrophil may represent a fifth aggregating receptor site, in addition to those that already have been proposed for CSa, F-Met-Leu-Phe, leukotriene B4 and PAF (FordHutchinson, 1981;O'Flaherty, et al, 1981a). The neutrophil aggregation phenomena is thought to be related to the ability of PMNs to adhere to the vascular endothelium (O'Flaherty et al, 1978).…”

Section: Discussionmentioning

confidence: 70%

“…These results suggest that stimulation of arachidonic acid metabolism to produce a potent aggregating agent such as leukotriene B4 may potentiate the responses to UTP and ATP but not to ADP. In this context it has been proposed that other aggregating agents may work in part through the release of leukotriene B4 (O'Flaherty, Hammett, Shewmake, Wykle, Love, McCall & Thomas, 1981a).…”

Section: Discussionmentioning

confidence: 99%

Aggregation of Rat Neutrophils by Nucleotide Triphosphates

FORD‐HUTCHINSON

1982

British J Pharmacology

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Adenosine 5′‐triphosphate (ATP) and uridine 5′‐triphosphate (UTP) at concentrations of 3 × 10−7 m and greater cause a rapid partially reversible aggregation of rat polymorphonuclear leucocytes. Other neucleotide phosphates are much less active at producing aggregation responses; the agonist potencies being UTP > ATP > guanosine 5′‐triphosphate, cytidine 5′‐triphosphate, thymidine 5′‐triphosphate; ATP > adenosine 5′‐diphosphate (ADP) > adenosine 5′‐monophosphate (AMP); and ADP > uridine 5′‐diphosphate, thymidine 5′‐diphosphate, guanosine 5′‐diphosphate, cytidine 5′‐diphosphate. Adenosine is inactive. The hydrolysis resistant analogues of ATP, α‐β‐methylene ATP and β‐γ‐methylene ATP, do not cause neutrophil aggregation suggesting that hydrolysis of ATP and UTP may be required to initiate the aggregation response. It is postulated that ATP and UTP may be important stimulants of neutrophil function and may be involved in the adherence of these cells to the vascular endothelium.

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Prostaglandins and Other Metabolites of Arachidonic Acid: An Overview for the Otolaryngologist

Poole¹,

Pillsbury²

1985

Archives of Otolaryngology - Head and Neck Surgery

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\s=b\Metabolites of arachidonic acid have important regulatory functions within several areas of concern to the otolaryngologist. Prostaglandins, composing one group, are involved in smooth-muscle contraction, regulation of renal glomerular blood flow, and in the modulation of immune and allergic responses, inflammation, fever, pain, and tumor growth. A second group, the leukotrienes, may be even more important than prostaglandins in allergy and inflammation. The elusive slow-reacting substance of anaphylaxis belongs in this group. Two other metabolites, thromboxane and prostacyclin, seem to be critical in hemostasis and the metastatic spread of tumors. (Arch Otolaryngol 1985;111:317-321) During the past ten years, research concerning prostaglandins and related substances has proceeded at a faster rate than any other branch of medical investigation. New com¬ pounds have been discovered, charac¬ terized, and pharmacologically ma¬ nipulated. The acquired information has rapidly moved from basic research into clinical usefulness.Since this new and complex area has profound relevance for virtually every area of medicine and surgery, we have conducted a brief review, focusing on the aspects that have particular appli¬ cation in our specialty. METABOLISM AND PHYSIOLOGYThe family of prostaglandinlike compounds might be more correctly referred to as metabolites of arachi¬ donic acid, with true prostaglandins making up only one subset. The prostaglandins are manufactured in all cells, with the exception of those cells lacking microsomes (eg, mature red blood cells). Different cell types, how¬ ever, show marked variation in the amounts and ratios of the different arachidonic acid products. In general, they function as locally active hor¬ mones, with rapid hepatic and pulmo¬ nary catabolism resulting in very short systemic half-lives.1The major metabolic pathways from arachidonic acid are depicted in the Figure. For simplicity, some of the intermediates and less well-known products have been omitted. Arachi¬ donic acid is formed through the action of phospholipases on mem¬ brane phospholipids, which are derived from polyunsaturated longchain fatty acids. In particular, linoleic acid is an essential fatty acid alleg¬ edly because of its role as an arachi¬ donic acid precursor.2 Once in the microsomes, arachidonic acid metabo¬ lism proceeds either along the cyclo-oxygenase pathway or along the lipoxygenase pathway.In the first pathway, cyclo-oxyge¬ nase catalyzes two consecutive steps that lead to the formation of true prostaglandins (PGs). The classes of the prostaglandins are designated by letter suffixes, ie, PGE, PGD, PGF. The degree of unsaturation in a given PG is then depicted by a numerical subscript, as in PGE^PGE2, and PGF2. The important cyclo-oxygenase activity first converts arachidonic acid and molecular oxygen to the cyclic endoperoxide PGG2 and subse¬ quently to PGH2 by hydroperoxidation.3 Then PGH2 can be metabolized to any of the more stable prostaglan¬ dins (PGE2, PGD2, or PGF2), thromboxane, or to prost...

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Evidence for 5,12-dihydroxy-6,8,10,14-eicosatetraenoate as a mediator of human neutrophil aggregation

Cited by 56 publications

References 12 publications

Acetyl glyceryl ether phosphorylcholine stimulates leukotriene B4 synthesis in human polymorphonuclear leukocytes.

Acetyl glyceryl ether phosphorylcholine stimulates leukotriene B4 synthesis in human polymorphonuclear leukocytes.

Aggregation of Rat Neutrophils by Nucleotide Triphosphates

Prostaglandins and Other Metabolites of Arachidonic Acid: An Overview for the Otolaryngologist

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