Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

Parillo, Francesco; Maranesi, Margherita; Mignini, Fiorenzo; Marinelli, Lisa; Stefano, Antonio Di; Boiti, Cristiano; Zerani, Massimo

doi:10.1371/journal.pone.0104797

Cited by 16 publications

(15 citation statements)

References 33 publications

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“…Following antioxidant administration, the murine ovarian transcriptome reflected an environment with enhanced cell survival and β-adrenergic signaling, decreased apoptotic signaling proinflammatory pathways, and altered antioxidant signaling compared to control aged mice. Upregulated β-adrenergic signaling may contribute to improved ovarian function through changes in follicular development and hormone secretion [37][38][39], particularly since ovarian sympathetic nerve activity may decline with ovarian aging [40]. The anti-inflammatory effects of antioxidant treatment are consistent with previous reports [41].…”

Section: Discussionsupporting

confidence: 81%

Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte

Katz‐Jaffe

Lane

Parks

et al. 2020

Life

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Advanced maternal age (AMA) is associated with reduced fertility due in part to diminished ovarian follicle quantity, inferior oocyte quality, chromosome aneuploidy, and lower implantation rates. Ovarian aging is accompanied by increased oxidative stress and blunted antioxidant signaling, such that antioxidant intervention could improve reproductive potential. The first aim of this study was to determine the molecular effects of antioxidant intervention in the ovaries and oocytes of aged mice, utilizing a supplement containing only naturally occurring açaí (Euterpe oleracea) with an oxygen radical absorbance capacity of 208,628 μmol Trolox equivalent (TE)/100 g indicating high antioxidant activity. Nine month old female CF-1 mice were administered 80 mg/day antioxidants (n = 12) or standard diet (n = 12) for 12 weeks. In the ovary, antioxidant treatment upregulated β-adrenergic signaling, downregulated apoptosis and proinflammatory signaling, and variably affected cell growth and antioxidant pathways (p < 0.05). Exogenous antioxidants also increased the oocyte expression of antioxidant genes GPX1, SOD2, and GSR (p < 0.05). A feasibility analysis was then conducted on female AMA infertility patients as a proof-of-principle investigation. Patients (n = 121; <45 years old) consented to receiving 600 mg antioxidants three times daily for ≥8 weeks preceding infertility treatment. Preliminary results indicate promising outcomes for AMA patients, warranting further investigation.

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Section: Discussionsupporting

confidence: 81%

Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte

Katz‐Jaffe

Lane

Parks

et al. 2020

Life

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“…The slope of these graphs was used to determine the reaction efficiency. Sample mRNA quantification was evaluated using iCycler system software, while mRNA gene expression was quantified using the 2 –ΔΔCt method [22,23]. The melting curve analysis was carried out, immediately after the PCR end cycle, in order to determine the specificity of each primer set.…”

Section: Methodsmentioning

confidence: 99%

Apelin System in Mammary Gland of Sheep Reared in Semi-Natural Pastures of the Central Apennines

Mercati

Maranesi

Dall’Aglio

et al. 2018

Animals

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Simple SummaryGrazing activity is fundamental to natural grassland biodiversity preservation. Increasing summer aridity decreases the grassland pastoral value, negatively affecting animal morpho-functional features and production with detrimental effects on the extensive sheep farming sustainability. Since adipokines represent a link between a subject’s energy availability and tissue metabolism, we investigated the presence and distribution of the system composed of the adipokine apelin and its receptor in the mammary gland of sheep during the period between maximum flowering and maximum dryness of the pasture, providing a group of sheep with food supplementation. This work represents a part of a wider study aimed to buffer the negative effects of increasing summer drought stress on farm income and to maintain the grassland biodiversity. Our findings improve the knowledge of apelin/receptor system function on the sheep mammary gland and this could be a useful tool in the farm management practices.AbstractSheep are the most bred species in the Central Italy Apennine using the natural pastures as a trophic resource and grazing activity is fundamental to maintain the grassland biodiversity: this goal can be reached only ensuring an economical sustainability to the farmers. This study aimed to investigate the apelin/apelin receptor system in ovine mammary gland and to evaluate the differences induced by food supplementation, in order to shed light on this system function. A flock of 15 Comisana x Appenninica adult dry ewes were free to graze from June until pasture maximum flowering (MxF). From this period to pasture maximum dryness (MxD), in addition to grazing, the experimental group (Exp) was supplemented with 600 g/day/head of cereals. Apelin and apelin receptor were assessed by Real-Time PCR and immunohistochemistry on the mammary glands of subjects pertaining to MxF, MxD and Exp groups. They were detected in alveolar and ductal epithelial cells. The pasture maximum flowering group showed significant differences in apelin expression compared with experimental and MxD groups. Apelin receptor expression significantly differed among the three groups. The reduced apelin receptor expression and immunoreactivity levels during parenchyma involution enables us to hypothesize that apelin receptor plays a modulating role in the system control.

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“…It was reported that DA/DR system exerts a dual modulatory function in the lifespan of corpora lutea (the DA/D1R as luteotropic and the DA/D3R as luteolytic). 70…”

Section: Resultsmentioning

confidence: 99%

“…It was reported that DA/DR system exerts a dual modulatory function in the lifespan of corpora lutea (the DA/D1R as luteotropic and the DA/ D3R as luteolytic). 70 Among the investigated molecules, aristola-1(10),8-dien, b-humulene, bicyclogermacrene, a-bisabolol oxide A, a-bisabolone oxide, coumarin-7-methoxy (herniarin, umbelliferone), b-eudesmol, ficusin, methyl jasmonate, nerolidol, p-cymen-8-ol, pinocarvone, spathulenol, thujone, (þ)-spathulenol, 7-epi-b-eudesmol, cis-a-bergamotene, trans-a-bergamotene, and (E)-methyl jasmonate (Figure 1) had a reasonable interaction with the dopamine receptors. The binding energy for the named ligands ranged from 88.60% to 100% of the cocrystallized ligand of the dopamine receptor.…”

Section: Dopamine Receptorsmentioning

confidence: 99%

An Assay on the Possible Effect of Essential Oil Constituents on Receptors Involved in Women’s Hormonal Health and Reproductive System Diseases

Sakhteman

Pasdaran

Afifi

et al. 2020

J Evid Based Complementary Altern Med

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Aromatic herbal remedies, hydrosols, and essential oils are widely used for women’s hormonal health. Scientific investigation of their major constituents may prevent unwanted infertility cases, fetal abnormalities, and drug-herb interactions. It also may lead to development of new medications. A list of 265 volatile molecules (mainly monoterpenes and sesquiterpenes) were prepared from a literature survey in Scopus and PubMed (2000-2019) on hydrosols and essential oils that are used for women’s hormonal and reproductive health conditions. The PDB (protein data bank) files of the receptors (136 native PDB files) that involve with oxytocin, progesterone, estrogen, prolactin, acetyl choline, androgen, dopamine, human chorionic gonadotropin, luteinizing hormone, follicle-stimulating hormone, aromatase, and HER2 receptors were downloaded from Protein Data Bank. An in silico study using AutoDock 4.2 and Vina in parallel mode was performed to investigate possible interactions of the ligands with the receptors. Drug likeliness was investigated for the most active molecules using DruLiTo software. Aristola-1(10),8-diene, bergapten (5-methoxypsoralen), α-bergamotene, bicyclogermacrene, α-bisabolol oxide A, α-bisabolone oxide, p-cymen-8-ol, 10-epi elemol, α-elemol, β-eudesmol, 7- epi-β-eudesmol, ficusin, β-humulene, methyl jasmonate, nerolidol, pinocarvone, (+)-spathulenol, and thujone had better interactions with some androgen, aromatase, estrogen, progesterone, HER2, AChR, and/or dopamine receptors. Most of these molecules had an acceptable drug likeliness except for α-bergamotene, bicyclogermacrene, β-humulene, and aristola-1(10),8-diene. Some volatile natural molecules can be considered as lead compound for drug development to treat hormonal conditions.

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Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

Cited by 16 publications

References 33 publications

Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte

Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte

Apelin System in Mammary Gland of Sheep Reared in Semi-Natural Pastures of the Central Apennines

An Assay on the Possible Effect of Essential Oil Constituents on Receptors Involved in Women’s Hormonal Health and Reproductive System Diseases

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