“…RAD-Seq has been successfully used to identify sex-specific sequences in non-model species from diverse taxa, including fish (Drinan, Loher, & Hauser, 2018) , amphibians (Bewick et al, 2013) , non-avian reptilia (Gamble, 2016;Gamble et al, 2017Gamble et al, , 2015Gamble et al, , 2018Gamble & Zarkower, 2014;S. V. Nielsen, Banks, Diaz, Trainor, & Gamble, 2018;Stuart V. Nielsen, Daza, Pinto, & Gamble, 2019) , invertebrates (Carmichael et al, 2013;Mathers et al, 2015;Pratlong et al, 2017) , and plants (Kafkas, Khodaeiaminjan, Güney, & Kafkas, 2015) , and to identify the sex chromosomes and the sex locus in some of these species (Wilson et al, 2014) . Although dedicated pipelines have been developed to analyze RAD-Seq data specifically for sex determination (Gamble & Zarkower, 2014) , most of the above mentioned studies have used STACKS (J.…”