Evidence for a hepatic‐renal antagonism in the production of hepatic erythropoietin in a phenylhydrazine‐induced compensated hemolytic state in the rat

Abstract

A compensated hemolytic state was induced in rats by injection of phenylhydrazine (PHZ) over a 6-week period. The liver and kidney were perfused to determine the levels and time of appearance of a hepatic erythropoietic factor (HEF) which induces the production of hepatic erythropoietin (Ep) and its antagonist, a renal inhibitory factor (RIF). Erythropoietin assays on the perfusates have been previously reported. The amount of HEF in perfusates recovered from the livers of the PHZ-treated rats was significantly higher during the 4th to 5th weeks of treatment, coinciding with the time of increased liver production of Ep. During the 6th week of PHZ treatment, the titer of RIF in perfusates recovered from the kidney was markedly increased and the HEF titer was decreased to near the control level, suggesting inhibitory action of RIF on HEF synthesis and/or effect. These findings indicate that an HEF/RIF control mechanism regulates hepatic Ep production in this compensated hemolytic condition.

Hematological effects of 1,3,5‐trinitrobenzene (TNB) in rats in vivo and in vitro

“…The hemolytic anemia observed with TNB is analogous to that reported with phenylhydrazine (PHZ). PHZ, like TNB, is a potent oxidant drug but does not cause hemolysis in vitro (Dornfest et al, 1983). It has been reported that PHZ can cross-link red-cell band 3 protein (senescent antigen), resulting in the binding of autologus immunoglobulin G (IgC) and complement deposition.…”

Bilirubin Lowering Potential of Aqueous Carica Papaya Extract in Induced Jaundice in Rats

“…Formation of Methaemoglobin and Heinz body formation are the other effects of PHZ toxicity [18]. PHZ is known for its ability to produce haemolysis in rats and humans [19][20][21]. PHZ is known to decrease haemoglobin levels, RBC count and PCV [18,22], however ACP (400 mg/kg and 800 mg/kg) especially the 800 mg/kg extract was able to significantly (p < 0.05) reverse the effect suggesting that ACP has the ability to boost red blood cell, haemoglobin and haematocrit levels as earlier demonstrated by a study conducted by Dharmarathna et al [23] on ACP, where it was observed that administration of ACP leaf extract caused a significant increase in the level of platelets and RBC in mice.…”

Broiler ascites: a review of the ascites work done at the poultry section of the Onderstepoort Veterinary Institute 1981–1990

“…Phenylhydrazine is used to induce anaemia in other species (Dornfest et al, 1983;Long, 1926. When we administered phenylhydrazine to broilers, the birds became anorexic and did not develop right ventricular hypertrophy (unpublished).…”