Evidence for a juvenile hormone receptor involved in protein synthesis in Drosophila melanogaster.

Shemshedini, Lirim; Lanoue, M.; Wilson, Thomas G.

doi:10.1016/s0021-9258(19)39917-x

Cited by 100 publications

(13 citation statements)

References 34 publications

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“…Its presence during the larval intermoult periods and absence at the time of pupal commitment by the epidermis suggests a mechanism by which tissues could regulate their sensitivity to JH (Palli et al, 1991a). In D. melanogaster a cytosolic JH binding protein (Ko = 6.7 nM for JHIII) has been found in several tissues presumed to be JH sensitive and not in tissues presumed to be insensitive (Shemshedini et al, 1990). Furthermore, the binding affinity of the protein for JHIII is 10-fold higher in strains which are susceptible to the JH analogue, methoprene than in mutants exhibiting resistance to methoprene, strongly suggesting that the binding protein is the JH receptor (Shemshedini and Wilson, 1990).…”

Section: Mechanisms For Jh Actionmentioning

confidence: 99%

“…The second mechanism by which JH appears to exert its influence on cells appears to be mediated by membrane protein/second messenger systems and involves activation of protein kinase C (Y amomoto et al, 1988;Sevala and Davey, 1989). However, the presumptive intracellular receptor was also found in one of these tissues, suggesting that both mechanisms of response to JH may operate in a single tissue (Shemshedini et al, 1990).…”

Section: Mechanisms For Jh Actionmentioning

confidence: 99%

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Characterisation of juvenile hormone esterase in Drosophila melanogaster

Campbell

Healy

Oakeshott

1992

Insect Biochemistry and Molecular Biology

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Juvenile hormone (JH) is one of the main morphogenic hormones of insects and degradation of the hormone is believed to play an important role in its regulation in many insects. The two main routes for JH degradation are hydroy sis by JH esterase (JHE) and JH epoxide hydrolase (JHEH). • A major focus of research has been on juvenile hormone esterase (JHE) found in the haemolymph of final instar lepidopteran larvae, although considerable hydrolysis of JH occurs in other tissues and at other life stages. In contrast, no comprehensive study of JH degradation has been undertaken in any dipteran species. However, the available evidence suggests that very little JH degrading activity is found in the haemolymph of Diptera and several dipteran JHEs exhibit atypical patterns of sensitivity to inhibitors. These data suggested that both the expression pattern and the biochemical properties of dipteran JH degrading enzymes may differ from other insect orders, and therefore, that the role of JH degradation may be different.The aim of this project was to comprehensively characterise JH degradation in the dipteran, Drosophila melanogaster, with special emphasis on JHE. The first objective was to identify the JH degrading enzymes of D . melanogaster and the second was to deterrnine their expression patterns and biochemical properties in order to determine thei~ role in the regulation of JH titre. These data were compared with other data to deterrnine whether a general model could be developed for dipteran JH titre regulation. Finally, the roles of JH hydrolysis in the regulation of JH titre were compared among Diptera, Lepidoptera and other insects.Two approaches were taken to identify the JH degrading enzymes of D. melanogaster. The first was to investigate the properties of an esterase, denoted EST20, which can be visualised after native polyacrylarnide gel electrophoresis using artificial esterase substrates, a-and B-naphthylacetate. EST20 is shown to be a likely homologue of the p-esterase of Drosophila virilis, which has been claimed to be the JHE of this species. However, this study shows that JH hydrolytic activity is associated with neither EST20 in D. melanogaster nor the p-esterase in D . virilis.The second approach was to use a radiometric assay which identified two JH hydrolysing enzymes, JHE and JHEH. The developmental profile of JH hydrolytic activity in D. melanogaster correlates inversely with profiles of JH titre and production. Key features are prewandering and prepupal maxima of JH hydrolytic activity in whole body homogenates of final instar larvae. This developmental profile of expression is similar to the lepidopteran pattern suggesting that the role of JH hydrolysis is essentially similar during this period. In D. melanogaster, the prewandering maximum consists mainly of JHEH whereas the prepupal peak consists mainly of JHE. However, the relative contributions of JHE and JHEH at these times differs both among the Diptera and in other orders.JHE from the prepupal stage was purified to homogeneity by selectiv...

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Section: Mechanisms For Jh Actionmentioning

confidence: 99%

Section: Mechanisms For Jh Actionmentioning

confidence: 99%

Characterisation of juvenile hormone esterase in Drosophila melanogaster

Campbell

Healy

Oakeshott

1992

Insect Biochemistry and Molecular Biology

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“…Juvenile hormone has previously been shown to promote AG growth (Herndon et al, 1997; Shemshedini et al, 1990; T. G. Wilson, DeMoor, & Lei, 2003; K. Yamamoto, Chadarevian, & Pellegrini, 1988). Here we show that endoreplication, gland size, and overall fertility are affected when this signaling pathway is disrupted.…”

Section: Discussionmentioning

confidence: 99%

“…Juvenile Hormone (JH) signaling has been shown to promote accessory gland growth and protein synthesis in Drosophila and other insects (Herndon et al, 1997; Shemshedini, Lanoue, & Wilson, 1990; R. Yamamoto, Bai, Dolezal, Amdam, & Tatar, 2013). To test whether JH signaling may play a role in main cell endocycling on the day of eclosion in Drosophila accessory glands, we expressed RNAis to the JH nuclear receptors, Gce (germ cell-expressed bHLH-PAS) or Met (Methoprene tolerant), or their co-activator Tai (Taiman).…”

Section: Introductionmentioning

confidence: 99%

Cell cycle variants duringDrosophilaaccessory gland development

Box

Church

Hayes

et al. 2019

Preprint

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The Drosophila melanogaster accessory gland is a functional analog of the mammalian prostate made up of two secretory epithelial cell types, termed main and secondary cells.This tissue is responsible for making and secreting seminal fluid proteins and other molecules that contribute to successful reproduction. Here, we show that similar to the mammalian prostate, this tissue grows with age. We find that the adult accessory gland grows in part via endocycles to increase DNA content and cell size. The differentiated, binucleated main cells remain poised to endocycle in the adult gland, and tissue damage or upregulation of signals that promote growth are sufficient to trigger dramatic endocycling leading to increases in cell size and ploidy, independent of mating status. Our data establishes that the adult accessory gland is not quiescent, but instead uses endocycles to maintain accessory gland function throughout the fruit fly's lifespan.

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“…Juvenile hormone evolves as a gonadotrophic hormone ( Dubrovsky et al, 2002 ; Riddiford, 2012 ; Santos et al, 2019 ), which has been implicated in vitellogenesis and yolk protein uptake in Drosophila females ( Postlethwait and Weiser, 1973 ; Bownes, 1989 ; Saunders et al, 1990 ; Soller et al, 1999 ; Riddiford, 2012 ), larval fat body histolysis ( Postlethwait and Jones, 1978 ; Yamamoto et al, 2013 ), and male accessory gland protein synthesis ( Yamamoto et al, 1988 ; Shemshedini et al, 1990 ; Wolfner et al, 1997 ; Wilson et al, 2003 ).…”

Section: Physiological Actions Of Juvenile Hormone Intracellular Sign...mentioning

confidence: 99%

Juvenile Hormone Studies in Drosophila melanogaster

Zhang

Liu

2022

Front. Physiol.

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In the field of insect endocrinology, juvenile hormone (JH) is one of the most wondrous entomological terms. As a unique sesquiterpenoid hormone produced and released by the endocrine gland, corpus allatum (CA), JH is a critical regulator in multiple developmental and physiological processes, such as metamorphosis, reproduction, and behavior. Benefited from the precise genetic interventions and simplicity, the fruit fly, Drosophila melanogaster, is an indispensable model in JH studies. This review is aimed to present the regulatory factors on JH biosynthesis and an overview of the regulatory roles of JH in Drosophila. The future directions of JH studies are also discussed, and a few hot spots are highlighted.

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Evidence for a juvenile hormone receptor involved in protein synthesis in Drosophila melanogaster.

Cited by 100 publications

References 34 publications

Characterisation of juvenile hormone esterase in Drosophila melanogaster

Characterisation of juvenile hormone esterase in Drosophila melanogaster

Cell cycle variants duringDrosophilaaccessory gland development

Juvenile Hormone Studies in Drosophila melanogaster

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