Evidence for a new post-translational modification in Staphylococcus aureus: Hydroxymethylation of asparagine and glutamine

Waridel, Patrice; Ythier, Mathilde; Gfeller, Aurélie; Moreillon, Philippe; Quadroni, Manfredo

doi:10.1016/j.jprot.2011.12.014

Cited by 6 publications

(2 citation statements)

References 25 publications

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“…A recent shotgun based high resolution LC-MS/MS approach involving the enrichment of surface proteins using “trypsin shaving” was applied to the opportunistic pathogen S. aureus toward the identification of hydroxymethylation on aspargine and glutamine amino acid residues in an attempt to identify the presence and potential regulatory importance of this PTM on surface proteins which are known to assist in the colonization and invasion of the host cell ( Waridel et al, 2012 ). The authors reported a total of 15 proteins (mostly surface proteins) that contained hydroxymethylation modifications and could play a role in virulence factor modulation.…”

Section: Post Translational Modifications (Ptms)mentioning

confidence: 99%

Mass Spectrometry-Based Bacterial Proteomics: Focus on Dermatologic Microbial Pathogens

Soufi

Soufi²

2016

Front. Microbiol.

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The composition of human skin acts as a natural habitat for various bacterial species that function in a commensal and symbiotic fashion. In a healthy individual, bacterial flora serves to protect the host. Under certain conditions such as minor trauma, impaired host immunity, or environmental factors, the risk of developing skin infections is increased. Although a large majority of bacterial associated skin infections are common, a portion can potentially manifest into clinically significant morbidity. For example, Gram-positive species that typically reside on the skin such as Staphylococcus and Streptococcus can cause numerous epidermal (impetigo, ecthyma) and dermal (cellulitis, necrotizing fasciitis, erysipelas) skin infections. Moreover, the increasing incidence of bacterial antibiotic resistance represents a serious challenge to modern medicine and threatens the health care system. Therefore, it is critical to develop tools and strategies that can allow us to better elucidate the nature and mechanism of bacterial virulence. To this end, mass spectrometry (MS)-based proteomics has been revolutionizing biomedical research, and has positively impacted the microbiology field. Advances in MS technologies have paved the way for numerous bacterial proteomes and their respective post translational modifications (PTMs) to be accurately identified and quantified in a high throughput and robust fashion. This technological platform offers critical information with regards to signal transduction, adherence, and microbial–host interactions associated with bacterial pathogenesis. This mini-review serves to highlight the current progress proteomics has contributed toward the understanding of bacteria that are associated with skin related diseases, infections, and antibiotic resistance.

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Section: Post Translational Modifications (Ptms)mentioning

confidence: 99%

Mass Spectrometry-Based Bacterial Proteomics: Focus on Dermatologic Microbial Pathogens

Soufi

Soufi²

2016

Front. Microbiol.

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“…These results suggested the observed hydroxymethylation is selective, dependent on growth conditions and therefore an authentic post-translational modification, rather than an unspecific process. Although the authors speculated that the modification may modulate staphylococcal virulence there is no direct data in favor of this hypothesis and the function of this modification remains unknown (Waridel et al, 2012). Glycosylation of S. aureus proteins was also recently studied.…”

Section: Proteomic Analysis Of Postranslational Modificationsmentioning

confidence: 99%

Proteomics in studies of Staphylococcus aureus virulence

2015

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Staphylococcus aureus is a widespread, opportunistic pathogen that causes community and hospital acquired infections. Its high pathogenicity is driven by multifactorial and complex mechanisms determined by the ability of the bacterium to express a wide variety of virulence factors. The proteome secreted into extracellular milieu is a rich reservoir of such factors which include mainly nonenzymatic toxins and enzymes. Simultaneously, membrane proteins, membrane-cell wall interface proteins and cell wall-associated proteins also strongly influence staphylococcal virulence. Proteomics shows a great potential in exploring the role of the extracellular proteome in cell physiology, including the pathogenic potential of particular strains of staphylococci. In turn, understanding the bacterial physiology including the interconnections of particular factors within the extracellular proteomes is a key to the development of the ever needed, novel antibacterial strategies. Here, we briefly overview the latest applications of gel-based and gel-free proteomic techniques in the identification of the virulence factors within S. aureus secretome and surfacome. Such studies are of utmost importance in understanding the host-pathogen interactions, analysis of the role of staphylococcal regulatory systems and also the detection of posttranslational modifications emerging as important modifiers of the infection process.

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Post-translational modification of ESKAPE pathogens as a potential target in drug discovery

Tiwari

2019

Drug Discovery Today

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Evidence for a new post-translational modification in Staphylococcus aureus: Hydroxymethylation of asparagine and glutamine

Cited by 6 publications

References 25 publications

Mass Spectrometry-Based Bacterial Proteomics: Focus on Dermatologic Microbial Pathogens

Mass Spectrometry-Based Bacterial Proteomics: Focus on Dermatologic Microbial Pathogens

Proteomics in studies of Staphylococcus aureus virulence

Post-translational modification of ESKAPE pathogens as a potential target in drug discovery

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