Evidence for a novel DNA component in chloroplasts of<i>Euglena gracilis</i>

Vandrey, Jobst P.; Stutz, Erhard

doi:10.1016/0014-5793(73)80452-1

Cited by 20 publications

(2 citation statements)

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“…Due to better storability (up to 2.5 months under refrigerated conditions) and reduced presence of polyphenols, mature leaves were preferred over young and tender leaves for the genomic DNA extraction from P. volubilis. The use of phenol: chloroform: isoamyl alcohol (25:24:1) mixture was effective in minimizing protein contamination in the DNA as was observed by Vandrey and Stutz (1973) in Euglena gracilis. The DNA extracted from all the populations was free from RNA and protein contamination, having OD260/280 values of 1.8-2.0.…”

Section: Standardization Of Dna Extraction Protocolmentioning

confidence: 99%

“…DNA extraction was done by modifying the CTAB protocol by Rogers and Benedich (1994). Phenol: Chloroform: Isoamyl alcohol (25:24:1) treatment was done as per Vandrey and Stutz (1973) to reduce protein contamination. The quality of DNA thus obtained was checked by electrophoresing (horizontal electrophoresis unit -BioRad, USA) in 0.8% agarose gel (in 1× TBE buffer), stained with ethidium bromide.…”

Section: Extraction Of Genomic Dna and Thermal Amplificationmentioning

confidence: 99%

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Genetic diversity of fragmented natural populations of Pyrenacantha volubilis Wight. in India

Ramachandran

Santhoshkumar

Mathew

et al. 2021

Preprint

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Pyrenacantha volubilis Wight. is a dioecious liana occurring in small, clustered populations. The species had no documented use until the recent past, when it was identified to be a source of a highly traded anti-cancer drug – camptothecin. In the present study, we examine the genetic diversity of 12 fragmented natural populations of P. volubilis using morphological and molecular traits. Twelve polymorphic Inter Simple Sequence Repeat (ISSR) primers and 29 agromorphological traits were used to discriminate the populations using UPGMA and NJ tree algorithms respectively. The ISSR amplicon profile had 133 distinct bands. The maximum number of amplicons were produced by UBC 844 (20 bands) and the average polymorphism was 80.07 per cent. The dendrograms obtained based on molecular and agro-morphological data are in close congruence. The Thiruvananthapuram population stood apart in both the analyses as a discrete outgroup: perhaps a consequence of local adaptation. Substantial genetic diversity exists among populations. This could be tapped in domestication, which is the only way forward for the long-term survival of this species. We also report for the first time a standardized method for extraction of genomic DNA from the leaves of P. volubilis.

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Section: Standardization Of Dna Extraction Protocolmentioning

confidence: 99%

Section: Extraction Of Genomic Dna and Thermal Amplificationmentioning

confidence: 99%