Evidence for a sodium-dependent sugar transport in rat tracheal epithelium

Saumon, Georges; Seigné, Eric; Clérici, Christine

doi:10.1016/0005-2736(90)90121-4

Cited by 10 publications

(2 citation statements)

References 14 publications

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“…Recent studies in both rodents [20] and humans [21] have demonstrated that AQP5 is present in the apical membranes of the surface epithelium of the proximal airways, in agreement with the finding of mercury-sensitive water channels in this region [26]. Another type of water permeation-associated with glucose transport-has been identified in tracheal and alveolar epithelia in man and in several animal species [10,12,15,32]. Thus, the family of glucose-transporters, including both uniport glucose transporters and the Na + /glucose cotransporter, is another mechanism that could be involved in airway transepithelial fluid transport.…”

Section: Introductionmentioning

confidence: 63%

Water permeability in human airway epithelium

Pedersen

Procida

Larsen

et al. 2005

Pflugers Arch - Eur J Physiol

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Osmotic water permeability (P(f)) was studied in spheroid-shaped human airway epithelia explants derived from nasal polyps by the use of a new improved tissue collection and isolation procedure. The fluid-filled spheroids were lined with a single cell layer with the ciliated apical cell membrane facing the outside. They were capable of surviving hours of experiment involving continuous superfusion of the bathing medium and changes of osmolarity. A new image analysis technique was developed for measuring the spheroid diameters, giving high time and measurement resolutions. The transepithelial P(f), determined by the changes of the apical solution osmolarity, was not influenced by the presence of glucose, Na(+), or Na(+)/glucose-cotransport inhibitors in the bath, but was sensitive to the aquaporin (AQP) inhibitor HgCl(2). The measured P(f) levels and the values of activation energy were in the range of those seen in AQP-associated water transport. Together, these results indicate the presence of an AQP in the apical membrane of the spheroids. Notably, identical values for P(f) were found in CF and non-CF airway preparations, as was the case also for the calculated spontaneous fluid absorption rates.

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Section: Introductionmentioning

confidence: 63%

Water permeability in human airway epithelium

Pedersen

Procida

Larsen

et al. 2005

Pflugers Arch - Eur J Physiol

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“…SGLT1 isoform has been indicated as the glucose/galactose transporter, localized in the brush border membrane of airway epithelial cells, which actively removes glucose from the ASL (Barker et al. 1989; Saumon et al. 1990, 1996; de Prost & Saumon, 2007).…”

Section: Introductionmentioning

confidence: 99%

Glucose transporter/T1R3‐expressing cells in rat tracheal epithelium

et al. 2012

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Glucose transport plays an important role in maintaining low sugar concentration in airway surface liquid (ASL), which is critical for mucociliary clearance and bacterial colonization. Experimental evidence indicates that glucose ⁄ hexose uptake in lung ⁄ airway cells occurs by means of two structurally distinct glucose transporter pathways: the Na + -dependent glucose transporters (SGLT family) and the facilitative glucose transporters (GLUT family). In this study, we examined the expression of the major glucose transporters of the intestine, GLUT2, GLUT5, SGLT1 and T1R3 taste receptor subunit, in the trachea of rats using immunohistochemistry and immunoelectron microscopy, and compared them using double-labeled confocal microscopy. We found that GLUT2, GLUT5, SGLT1 and T1R3 are selectively expressed in different cell types. T1R3 and GLUT2 are predominantly expressed in subsets of solitary chemoreceptor cells (SCCs) and ciliated cells, GLUT5 is present in subsets of SCCs and in secretory cells, and SGLT1 is exclusively expressed in a unique cell type, SCCs. Furthermore, we demonstrated that T1R3 is colocalized with SGLT1 in SCCs and with GLUT2 transporter in ciliated cells. In conclusion, these findings reveal that different cell types are associated with the uptake of glucose in ASL and that, due to their T1R3 expression, SCCs and ciliated cells are most likely to participate in the chemosensory process in ASL.

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Luminal glucose enhances transepithelial Na+ and fluid transports in rat lungs

Saumon

Dreyfuss

1991

Pfl�gers Arch

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The effects of luminal glucose on transepithelial Na+ and fluid transports were investigated in rat lungs. Two preparations were used: isolated, perfused lungs and lungs in situ perfused with blood (cross-circulations), a situation more comparable to that existing in vivo. Unidirectional (efflux from air spaces, J(out)) and net (Jnet) Na+ fluxes and fluid absorption from air spaces were estimated in lungs filled with bicarbonate-buffered solutions containing 10 mmol/l of either mannitol, glucose or alpha-methyl-D-glucopyranoside, or 0.1 mmol/l phlorizin in the presence of glucose. In the presence of mannitol J(out) was estimated to be 7.8 +/- 1.02 pmol cm-2 s-1 in isolated lungs and 9.2 +/- 0.97 pmol cm-2 s-1 in lungs in situ, and Jnet 1.0 +/- 0.33 and 2.5 +/- 0.35 pmol cm-2 s-1, respectively. When glucose replaced mannitol J(out) (+ 30% + 40%), Jnet (+200% + 300%) and fluid absorption (+ 100% + 400%) were enhanced in both preparations. Substituting methyl glucoside for mannitol increased Na+ and fluid absorption rates to the same extent as glucose. Phlorizin, in the presence of glucose, reduced Na+ and fluid transports to values similar to those observed in the presence of mannitol. These changes did not result from modifications of the paracellular permeabilities (assessed with [3H]mannitol).(ABSTRACT TRUNCATED AT 250 WORDS)

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Evidence for a sodium-dependent sugar transport in rat tracheal epithelium

Cited by 10 publications

References 14 publications

Water permeability in human airway epithelium

Water permeability in human airway epithelium

Glucose transporter/T1R3‐expressing cells in rat tracheal epithelium

Luminal glucose enhances transepithelial Na+ and fluid transports in rat lungs

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