Evidence for a Specific Microwave Radiation Effect on the Green Fluorescent Protein

Copty, A.; Neve-Oz, Yair; Barak, I.; Golosovsky, M.; Davidov, D.

doi:10.1529/biophysj.106.084111

Cited by 51 publications

(38 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MW radiation effect observed here is probably mediated by the water molecules attached to the protein surface. The protein bound water absorbs MW radiation in the range of 1 -15 GHz [31,32] as compared to the free water molecules that absorb at 19 GHz [1]. The MW frequency of 2.45 GHz used in this investigation is thus fall in the range for protein bound water absorption band.…”

Section: Resultsmentioning

confidence: 97%

“…Microwave radiation (MW) is a non-ionizing electromagnetic radiation growingly used for investigating the properties of biomaterials due to its localized and rapid action of transmitting the energy in aqueous environment [1]. The MW energy is transmitted in the form of heat, while the parallel concept of athermal effect of MW is a subject of much discussion [2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…The MW has only few reports on its effect on protein conformation and most of these studies focus on the irreversible effects on proteins [9][10][11][12]. Moreover, the MW-based denaturation studies are carried out with small proteins [1,7,9,11]; while the similar studies on large proteins of multimeric nature is not known. We report here the effect of MW radiation on the structure of a homo octameric Alcohol Oxidase (AO-x).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microwave based reversible unfolding and refolding of alcohol oxidase protein probed by fluorescence and circular dichroism spectroscopy

Chinnadayyala¹,

Santhosh²,

Goswami³

2012

JBPC

View full text Add to dashboard Cite

The reversible effect of microwave mediated denaturation of protein at low exposure time of 10 s has been demonstrated for the first time. The effect of microwave (2.45 GHz and 900 W) was confirmed in a homo-octameric alcohol oxidase in aqueous solution of pH 7.5. The unfolding events did not transverse through any intermediate states and no subunits of the protein were detached during the process. The refolding of the protein achieved at 4˚C for 24 h had regenerated the native enzyme. This reversible refolding approach excludes any chemical reagent and therefore established as simple technique for protein unfolding-folding studies.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microwave based reversible unfolding and refolding of alcohol oxidase protein probed by fluorescence and circular dichroism spectroscopy

Chinnadayyala¹,

Santhosh²,

Goswami³

2012

JBPC

View full text Add to dashboard Cite

show abstract

“…There is a study showing that microwaves offer a clean, rapid, convenient, and cost-effective method for heating that can cause an athermal effect by polarising macromolecules . Thus, it is increasingly used for investigating the properties of biomaterials (Copty et al 2006).…”

mentioning

confidence: 99%

Effect of microwave modification on mechanical properties and structural characteristics of soy protein isolate and zein blended film

Wang¹,

Gao²,

Wang³

et al. 2016

Czech J. Food Sci.

View full text Add to dashboard Cite

Wang N., Gao Y.Z., Wang P., Yang S., Xie T.M., Xiao Z.G. (2016): Effect of microwave modification on mechanical properties and structural characteristics of soy protein isolate and zein blended film. Czech J. Food Sci., 34: 180-188.Soy protein isolate (SPI) and zein were blended at different ratios, and native and microwave-modified films were prepared at pH 12 in an ethanol/water (20 : 80) mixture. The effect of the microwave modification on the mechanical properties and structural characteristics of the SPI and zein blended films was investigated. The results show that the microwave-modified blended film of 3 : 1 SPI-zein demonstrated the best mechanical properties: the highest breaking strength (2900 g) and the highest fracturing distance (16.08 mm). The morphology of the microwave-modified film was more homogeneous and had fewer pinholes than the zein-only film. In addition, the glass transition temperature of the microwave-modified film rose by nearly half compared to the zein-only film, while the melting temperature increased by almost 2.5%. The secondary structure analysis indicates that a moderate amount of microwave treatment will promote a decrease in α-helix, β-turn, and random coil content and an increase in β-sheet content. This study provides an edible material with better flexibility for food packaging.

show abstract

“…In order to perform temperature measurements at the cellular level, further experiments based on the use of chemical reactions (42) and microfluorimetry techniques (43) would be carried out. Applied and Environmental Microbiology…”

mentioning

confidence: 99%

Thermal and Nonthermal Effects of Discontinuous Microwave Exposure (2.45 Gigahertz) on the Cell Membrane of Escherichia coli

Rougier

Prorot

Chazal

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

The aim of this study was to investigate the effects on the cell membranes of Escherichia coli of 2.45-GHz microwave (MW) treatment under various conditions with an average temperature of the cell suspension maintained at 37°C in order to examine the possible thermal versus nonthermal effects of short-duration MW exposure. To this purpose, microwave irradiation of bacteria was performed under carefully defined and controlled parameters, resulting in a discontinuous MW exposure in order to maintain the average temperature of the bacterial cell suspensions at 37°C. Escherichia coli cells were exposed to 200-to 2,000-W discontinuous microwave (DW) treatments for different periods of time. For each experiment, conventional heating (CH) in a water bath at 37°C was performed as a control. The effects of DW exposure on cell membranes was investigated using flow cytometry (FCM), after propidium iodide (PI) staining of cells, in addition to the assessment of intracellular protein release in bacterial suspensions. No effect was detected when bacteria were exposed to conventional heating or 200 W, whereas cell membrane integrity was slightly altered when cell suspensions were subjected to powers ranging from 400 to 2,000 W. Thermal characterization suggested that the temperature reached by the microwave-exposed samples for the contact time studied was not high enough to explain the measured modifications of cell membrane integrity. Because the results indicated that the cell response is power dependent, the hypothesis of a specific electromagnetic threshold effect, probably related to the temperature increase, can be advanced.T he interaction of electromagnetic fields (EMFs) and various life processes has been studied and debated for more than half a century. Identifying and evaluating the biological effects of microwaves (MW) is complex and controversial. Whereas one of the current theories is that heat generation induced by microwaves is responsible for biological effects, there has been a persistent view in the physical and engineering sciences that microwave fields are unable to induce bioeffects other than by heating (1) (2). Because of the scarcity of information on the mechanism of interaction between microwave and biological systems, this controversy endures.A great number of studies of the thermal versus nonthermal bioeffects of low-power MW were performed with various cellular functions, including gene expression (3) and mutation (4), enzyme activity (5), unfolding of proteins (6), biochemical cell systems (7), cell wall (8), cell morphology (9), and cell proliferation (10-13). Whereas several authors showed nonthermal effects, safety standards have been set based solely upon the thermal effect of MW. The main reason was that no satisfactory mechanism was proposed to explain the nonthermal bioeffects.When applied at high power, MW bioeffects induced by heating constitute one of the modern approaches for sterilization and decontamination processes in the food industry. In fact, microwaves have long been known to i...

show abstract

Evidence for a Specific Microwave Radiation Effect on the Green Fluorescent Protein

Cited by 51 publications

References 40 publications

Microwave based reversible unfolding and refolding of alcohol oxidase protein probed by fluorescence and circular dichroism spectroscopy

Microwave based reversible unfolding and refolding of alcohol oxidase protein probed by fluorescence and circular dichroism spectroscopy

Effect of microwave modification on mechanical properties and structural characteristics of soy protein isolate and zein blended film

Thermal and Nonthermal Effects of Discontinuous Microwave Exposure (2.45 Gigahertz) on the Cell Membrane of Escherichia coli

Contact Info

Product

Resources

About