Evidence for an Unconventional Radiosensitivity of Rat 9L Subcutaneous Tumors

Identification of hypoxia in cells and tissues of epigastric 9L rat glioma using EF5 [2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide]

“…The 9L glioma has been used extensively as a model for studies of radiotherapy (Leith et al, 1975;Wallen et al, 1980). Our data support the resistant nature of the 9L glioma shown by previous investigators (Leith et al, 1975;Wallen et al, 1980).…”

“…Recently, this tumour has been described as a tissue isolate grown on the epigastric branch of the femoral vessels (Evans and Koch, 1994). Typically, intracerebral and small subcutaneous 9L tumours are characterised as having minimal necrosis and no severe hypoxia (Leith et al, 1975;Wallen et al, 1980). However the 9L glioma has also been reported to contain uniform or moderate, intermittent hypoxia (Moulder and Rockwell, 1984;Wong et al, 1990;Franko et al, 1992).…”

“…After successive in vivo and in vitro transfers, a cell line was established (9L) which produced a gliosarcoma when implanted intracerebrally. Since that time, the 9L has been used extensively as a subcutaneous and intracerebral tumour model, especially for studies of radiosensitivity (Leith et al, 1975;Wallen et al, 1980). Recently, this tumour has been described as a tissue isolate grown on the epigastric branch of the femoral vessels (Evans and Koch, 1994).…”

Cell cycle distribution of hypoxia and progression of hypoxic tumour cells in vivo

“…However, when some specific tumours were compared at different sizes, there was a trend for the hypoxic fraction to increase as size increased, although this was not true for all tumour types (Stanley et al, 1977;Siemann, 1980;Wallen et al, 1980;Okunieff et al, 1986;Fu et al, 1990). Over the size range of 4-to 12-mm diameter, SaF, CaNT and Rh tumours showed no correlation between size and hypoxic fraction measured by flow cytometric analysis of bioreductively bound NITP abducts (Figure 2).…”

Hypoxic cells and in situ chemopotentiation of the nitrosoureas by misonidazole

“…(Barker et al, 1973;Wheeler et al, 1979) and s.c. tumours, and the in situ growth characteristics CCNU (4,8,12,16,or 20mgkg-1) either alone or simultaneously with 2.5 mmol kg-1 (0.5mg kg-1) of MISO. After 20-24 h, the rats were killed by cervical dislocation, the tumours removed and assayed for colony formation as previously described (Rosenblum et al, 1975a;Wallen et al, 1980). Tumours from rats injected with saline, MISO alone or HPMC were handled similarly and assayed in each experiment.…”