Evidence for ATP as a cotransmitter in dog mesenteric artery

Machaly, M.; Dalziel, H. H.; Sneddon, Peter

doi:10.1016/0014-2999(88)90636-x

Cited by 54 publications

(39 citation statements)

References 25 publications

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“…Modulation of ATP release by noradrenaline can only be revealed during nerve stimulation of very low frequencies (0.2±2 Hz) and short duration (10 s) [9,117]. Parallel with what has been described on transmitter release, the neurogenic contractions induced by electrical field stimulation of postganglionic sympathetic nerves within splanchnic vessels are biphasic [16,94,144,154]. In dog [115] and rabbit mesenteric arteries [181], the first component is purinergic in nature, develops rapidly, is transient and predominates at low frequencies (1-2 Hz) of stimulation, whereas the second component is adrenergic, develops after the purinergic one, is more long-lasting and becomes more evident as the frequency of stimulation increases (10 Hz).…”

Section: Effects Mediated By P2x and P2y Receptors In The Splanchnic mentioning

confidence: 83%

“…In what concerns the splanchnic circulation, the first description of such corelease was reported by Su [164] in the rabbit portal vein. Since then, growing evidence emerged that ATP is a cotransmitter in the splanchnic circulation of the dog [9,94,113,114,144,170], rabbit [140,181], guinea-pig [8], and rat [19,37,44].…”

Section: Effects Mediated By P2x and P2y Receptors In The Splanchnic mentioning

confidence: 99%

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Purinergic receptors in the splanchnic circulation

Morato

Sousa

Albino‐Teixeira

2008

Purinergic Signalling

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There is considerable evidence that purines are vasoactive molecules involved in the regulation of blood flow. Adenosine is a well known vasodilator that also acts as a modulator of the response to other vasoactive substances. Adenosine exerts its effects by interacting with adenosine receptors. These are metabotropic G-protein coupled receptors and include four subtypes, A 1 , A 2A , A 2B and A 3 . Adenosine triphosphate (ATP) is a co-transmitter in vascular neuroeffector junctions and is known to activate two distinct types of P2 receptors, P2X (ionotropic) and P2Y (metabotropic). ATP can exert either vasoconstrictive or vasorelaxant effects, depending on the P2 receptor subtype involved. Splanchnic vascular beds are of particular interest, as they receive a large fraction of the cardiac output. This review focus on purinergic receptors role in the splanchnic vasomotor control. Here, we give an overview on the distribution and diversity of effects of purinergic receptors in splanchnic vessels. Pre-and post-junctional receptormediated responses are summarized. Attention is also given to the interactions between purinergic receptors and other receptors in the splanchnic circulation.

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Section: Effects Mediated By P2x and P2y Receptors In The Splanchnic mentioning

confidence: 83%

Section: Effects Mediated By P2x and P2y Receptors In The Splanchnic mentioning

confidence: 99%

Purinergic receptors in the splanchnic circulation

Morato

Sousa

Albino‐Teixeira

2008

Purinergic Signalling

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“…The method for de sensitizing P2-purinoceptors with a, j3-methy lene-ATP has become widely accepted in recent years, and lends support to the hypothesis that ATP acts as a cotransmitter along with norepinephrine in several vascular tissues (Kennedy et al, 1986;War land and Burnstock, 1987;Machaly et al, 1988). Since the first observation by Needleman and co workers (1974) that ADP and ATP stimulate the re lease of prostaglandins in several organs, while adenosine and AMP do not, the relationship be tween P2-purinoceptors and prostaglandins has been confirmed in many studies (see Burnstock and Brown, 1981).…”

Section: Discussionmentioning

confidence: 94%

Heterogeneity of P₂-Purinoceptors in Brain Circulation

Torregrosa

Miranda

Salom

et al. 1990

J Cereb Blood Flow Metab

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Summary:The existence of P2-purinoceptors in the cere brovascular bed was examined by testing the effects of ATP and its stable analog, u,p-methylene-ATP, on CBF in the unanesthetized goat as well as on isometric tension in isolated goat middle cerebral artery. When injected directly into the cerebral circulation, A TP increased and u,p-methylene-ATP decreased CBF. Indomethacin did not modify either of these effects. The vasoconstrictor action of u,p-methylene-ATP was reduced by nicar dipine. "In vitro," both ATP and u,p-methylene-ATP contracted the cerebral arteries at resting tone, but the analog was more potent than A TP. 572cantly reduced the ATP-induced contractions. Nicar dipine inhibited both the u,p-methylene-ATP-and the ATP-induced contractile response. In preconstricted ar teries, ATP produced relaxation and u,p-methylene-ATP induced further contraction. The relaxant response to ATP was not modified by indomethacin. These results show the existence of two subtypes of P2-purinoceptors in brain circulation: P2x, more sensitive to u,P methylene-ATP than to ATP, which elicits cerebral vas oconstriction; and P2y, sensitive to ATP, which elicits cerebral vasodilation.

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“…The selectivivity of mATP has previously been established in the guinea-pig urinary bladder (Kasakov & Burnstock, 1982), chick rectum (Meldrum & Burnstock, 1985), rodent vas deferens Sneddon & Burnstock, 1984a), dog mesenteric artery (Muramatsu, 1986;Machaly et al, 1988), rabbit mesenteric artery (Kugelgen & Starke 1985;Ramme et al, 1987) and rat tail artery (Sneddon & Burnstock, 1984b).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, mATP has been used extensively in vitro to examine cotransmission by noradrenaline (NA) and ATP in a variety of sympathetically innervated tissues, including vascular smooth muscle (see for example Sneddon & Burnstock, 1984;Kennedy et al, 1986;Machaly et al, 1988). There is evidence that in some vessels, such as the rabbit saphenous artery, most of the sympathetic vasoconstriction is mediated by ATP acting on P21-purinoceptors (Burnstock & Warland, 1987).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the selectivity of α, β‐methylene ATP in inhibiting vascular responses of the rat in vivo and in vitro

Dalziel

Gray

Drummond

et al. 1990

British J Pharmacology

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1 The proposal that a,fi-methylene adenosine 5'-triphosphate (mATP) inhibits pressor responses in the pithed rat by selective desensitization of P2.-purinoceptors was examined by comparing the selectivity of its inhibitory effect on vascular responses in vitro and in vivo.2 In isolated ring preparations of rat femoral and tail artery, which had been denuded of endothelium, mATP markedly reduced the contractile response to exogenous ATP but had no effect on the response of the arteries to exogenous noradrenaline (NA). 3 In the pithed rat a substantial proportion of the pressor response to sympathetic nerve stimulation was resistant to a-adrenoceptor blockade, suggesting a non-adrenergic component to the sympathetic vasoconstriction. 4 In the pithed rat, repeated administration of desensitizing doses of mATP attenuated the pressor response to sympathetic nerve stimulation by approximately 80%, suggesting that a component of the sympathetic vasoconstriction is mediated by ATP acting on vascular P21-purinoceptors. However, the same mATP treatment also attenuated, to a similar degree, the pressor responses to intravenous NA, angiotensin II and vasopressin, indicating that the desensitization procedure was non-selective.5 These results demonstrate that while mATP can be used to desensitize selectively P2.-purinoceptors in vitro, its attenuation of the sympathetic nerve-mediated pressor response in vivo is non-selective.

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Evidence for ATP as a cotransmitter in dog mesenteric artery

Cited by 54 publications

References 25 publications

Purinergic receptors in the splanchnic circulation

Purinergic receptors in the splanchnic circulation

Heterogeneity of P₂-Purinoceptors in Brain Circulation

Investigation of the selectivity of α, β‐methylene ATP in inhibiting vascular responses of the rat in vivo and in vitro

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Evidence for ATP as a cotransmitter in dog mesenteric artery

Cited by 54 publications

References 25 publications

Purinergic receptors in the splanchnic circulation

Purinergic receptors in the splanchnic circulation

Heterogeneity of P2-Purinoceptors in Brain Circulation

Investigation of the selectivity of α, β‐methylene ATP in inhibiting vascular responses of the rat in vivo and in vitro

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Heterogeneity of P₂-Purinoceptors in Brain Circulation