Evidence for co-selection of antibiotic resistance genes and mobile genetic elements in metal polluted urban soils

Zhao, Yi; Cocerva, Tatiana; Cox, Siobhan; Tardif, Stacie; Su, Jian; Zhu, Yanmin; Brandt, Kristian K.

doi:10.1016/j.scitotenv.2018.11.372

Cited by 205 publications

(90 citation statements)

References 54 publications

(61 reference statements)

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“…Our results suggest that fecal contribution was the major driver of increased abundance of intl1 and ARGs included in this study. This conclusion offers a different scenario from what has been suggested in other studies that favor metals as selective agents of microbial antibiotic resistance (12-15), through co-selection process in the environment (11). However, it is important to note that fecal pollution was not considered in any of these studies.…”

Section: Discussioncontrasting

confidence: 70%

“…Urban soil samples of Belfast in Northern Ireland, exhibited a pattern of co-occurrence between metals (Zn, Cu, Cd, Co, Ni, Hg, Cr and As) and many ARGs. Moreover, the degree of metal toxicity was positively correlated with the abundance of MGEs, and ARGs (12). Metals, as Cu and Zncan in some cases exert stronger selection pressure over soil microbial communities for the selection of resistant bacteria, even more than specific antibiotics (13).…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic resistance genes and class 1 integron: Evidence of fecal pollution as a major driver for their abundance in water and sediments impacted by metal contamination and wastewater in the Andean region of Bolivia

Agramont

Gutiérrez-Cortez

Joffré

et al. 2020

Preprint

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26Water and sediment samples affected by mining activities were collected from 27 three lakes in Bolivia, the pristine Andean lake Pata Khota, the Milluni Chico lake 28 directly impacted by acid mine drainage, and the Uru-Uru lake located close to 29 Oruro city and highly polluted by mining activities and human wastewater 30 discharges. Physicochemical parameters, including metal compositions, were 31 analyzed in water and sediment samples. Antibiotic resistance genes (ARGs), 32 were screened for, and verified by quantitative PCR together with the mobile 33 element class 1 integron (intl1) as well as crAssphage, a marker of human fecal 34 pollution. The gene intl1 showed a positive correlation with sul1, sul2, tetA and 35 blaOXA-2. CrAssphage was only detected in Uru-Uru lake and its tributaries and 36 significantly higher abundance of ARGs were found in these sites. Multivariate 37 analysis showed that crAssphage abundance, electrical conductivity and pH were 38 positively correlated with higher levels of intl1 and ARGs. Taken together our 39 results suggest that fecal pollution is the major driver of higher ARGs and intl1 in 40 wastewater and mining contaminated environments. 41 3 48 can be incorporated and replicated in environmental microorganisms, thereby 49 increasing their concentration (3). 50 It has been reported that anthropogenic activities cause pollution of aquatic 51 environments with ARGs and MGEs (4). Wastewater discharges cause co-52 occurrence of MGEs and different ARGs in water and sediments (5). At a 53 continental scale, ARGs in sediments are strongly correlated with MGEs and 54antibiotic residues (6). Recently, it has been observed that microorganisms living in 55 aquatic microbial communities that came from wastewater were able to transfer 56 ARGs via horizontal gene transfer (HGT) after exposure to low levels of antibiotics 57 and biocides (7). Many of the ARGs that can be found in clinical settings have also 58 been found in the environment, suggesting the possibility of movement and 59 dissemination between these two scenarios (8). 60Mining activities cause contamination of downstream water with dissolved 61 metals (9) where heavy metals tend to accumulate in sediments (10). It has been 62 suggested that heavy metals can favor selection of ARGs via co-selection, i.e. the 63 simultaneous acquisition of both, ARG and metal resistance genes, where the 64 presence of metals constitutes the selective pressure (11). Several studies support 65 this relation. Urban soil samples of Belfast in Northern Ireland, exhibited a pattern 66 of co-occurrence between metals (Zn, Cu, Cd, Co, Ni, Hg, Cr and As) and many 67 ARGs. Moreover, the degree of metal toxicity was positively correlated with the 68 abundance of MGEs, and ARGs (12). Metals, as Cu and Zncan in some cases 69 exert stronger selection pressure over soil microbial communities for the selection 70 of resistant bacteria, even more than specific antibiotics (13). In the Dongying river 71 in China, the levels of Cu and Cr were positively c...

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Section: Discussioncontrasting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance genes and class 1 integron: Evidence of fecal pollution as a major driver for their abundance in water and sediments impacted by metal contamination and wastewater in the Andean region of Bolivia

Agramont

Gutiérrez-Cortez

Joffré

et al. 2020

Preprint

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“…The increase in MRGs induced by heavy metal pressure could facilitate the proliferation of ARGs. Moreover, coupling of genes for multidrug and multi-metals resistance were abundant, suggesting that many more types of antibiotic and metal resistance could be subjected to heavy metal selection pressure [5,12,20,23].…”

Section: Heavy Metals Induced Co-selection For Argsmentioning

confidence: 99%

“…The increasing spread and accumulation of antibiotic resistance genes (ARGs) in the environment due to unregulated use pose risks to human/animal health and challenge life-saving antibiotic therapies, which have raised global concerns [1][2][3][4]. Antibiotic resistance is prevalent in many natural environments including soil [5][6], surface water [7], groundwater [8], air [9], even in the gut microbiota [10][11]. The dispersal of ARGs in these environments presently is mainly associated with the improper disposal of environmental wastes containing contaminants as products of anthropogenic activities [5,[12][13].…”

Section: Introductionmentioning

confidence: 99%

“…In the environment, determinants of antibiotic resistance triggering the occurrence and proliferation of ARGs are complex [12,[18][19]. Among these, heavy metal contamination is one of the most important factors in uencing ARGs [5,12,20]. Heavy metals can co-select for ARGs and metal resistance genes (MRGs) using several mechanisms, such as co-resistance [21].…”

Section: Introductionmentioning

confidence: 99%

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Heavy Metal-Induced Co-Selection for Antibiotic Resistance in Terrestrial Subsurface Soils

Wang¹,

Lan²,

Fei³

et al. 2020

Preprint

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Background: Terrestrial surface ecosystems are important sinks for antibiotic resistance genes (ARGs) due to the continuous discharge of contaminants from human-impacted ecosystems. However, factors determining the abundance and resistance types of ARGs in terrestrial subsurface soils remain largely unknown. In this study, we investigated the abundance and diversity of ARGs, and their correlations with metal resistance genes (MRGs), mobile genetic elements (MGEs), bacteria, and heavy metals in subsurface soils in a global scale using high throughput quantitative PCR and metagenomic sequencing approaches. Results: Abundant and diverse ARGs were detected with high spatial heterogeneity among the sampling sites. Vertically, there was no significant difference in the ARG profiles between the aquifer and non-aquifer soils. Heavy metals were the key factors shaping ARG patterns in soils with high heavy metal contents, while they induced no significant effect in low contents. Moreover, heavy metals could trigger the proliferation of antibiotic resistance by increasing MGE abundance or influencing bacterial communities. Metagenomic analysis also revealed the widespread co-occurrence of ARGs and MRGs, with heavy metals possibly aggravating the co-selection of ARGs and MRGs in soils with high heavy metal contents. Conclusions: This study highlighted the heavy metal-induced co-selection for ARGs and MRGs and revealed the occurrence of ARG pollution in terrestrial subsurface soils.

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Antibiotic Resistance in Soil

Wang

Tiedje

2020

The Handbook of Environmental Chemistry

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Evidence for co-selection of antibiotic resistance genes and mobile genetic elements in metal polluted urban soils

Abstract: Evidence for co-selection of antibiotic resistance genes and mobile genetic elements in metal polluted urban

Cited by 205 publications

References 54 publications

Antibiotic resistance genes and class 1 integron: Evidence of fecal pollution as a major driver for their abundance in water and sediments impacted by metal contamination and wastewater in the Andean region of Bolivia

Antibiotic resistance genes and class 1 integron: Evidence of fecal pollution as a major driver for their abundance in water and sediments impacted by metal contamination and wastewater in the Andean region of Bolivia

Heavy Metal-Induced Co-Selection for Antibiotic Resistance in Terrestrial Subsurface Soils

Antibiotic Resistance in Soil

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