Evidence for cycle-dependent expression of full-length human chorionic gonadotropin/luteinizing hormone receptor mRNA in human endometrium and decidua

Licht, Peter B.; Wolff, Michael von; Berkholz, Alexander; Wildt, L.

doi:10.1016/s0015-0282(02)04822-7

Cited by 85 publications

(95 citation statements)

References 27 publications

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“…In fact, it has been shown that hCG administration during the secretory phase significantly modulates several endometrial paracrine parameters that are correlative with endometrial differentiation (eg, insulin‐like growth factor‐binding protein 1), angiogenesis (eg, vascular endothelial growth factor), implantation (eg, LIF, macrophage colony stimulating factor), and tissue remodeling (eg, matrix metallopeptidase 9) 14. Several studies have shown that the LH and hCG receptors are present in the human endometrium 11, 12. Furthermore, hCG has been detected at various levels in blastocyst culture media 15, 16.…”

Section: Discussionmentioning

confidence: 99%

“…It also has been reported that the specific interaction of blastocyst‐derived hCG and the endometrial luteinizing hormone (LH) and hCG receptors constitutes a fundamental component of the molecular dialogue at the materno‐fetal interface 9. Although the controversy that has been raised regarding the expression of the LH and hCG receptors in the human endometrium remains,10 several studies have shown that the LH and hCG receptors are present in the human endometrium 11, 12. In consideration of these reports, it seems that hCG that is used in luteal support might have a direct effect on the endometrium at implantation.…”

Section: Introductionmentioning

confidence: 99%

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Is human chorionic gonadotropin supplementation beneficial for frozen and thawed embryo transfer in estrogen/progesterone replacement cycles?: A randomized clinical trial

Shiotani

Matsumoto

Okamoto

et al. 2017

Reprod Medicine & Biology

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AimHuman chorionic gonadotropin (hCG) is used frequently for luteal support in fresh in vitro fertilization cycles as it induces progesterone secretion from the ovaries after oocyte retrieval and modulates the endometrium for implantation in fresh cycles. In contrast, hCG is not usually used for the transfer of cryopreserved‐thawed embryos in estrogen/progesterone replacement cycles because ovulation is suppressed. However, several studies have shown that luteinizing hormone and hCG receptors are present in the human endometrium and that hCG can directly induce the decidualization of endometrial stromal cells in vitro. Thus, this study evaluated whether hCG supplementation can be beneficial for cryopreserved‐thawed embryo transfer in estrogen/progesterone replacement cycles.MethodsOne‐hundred‐and seventy‐three cryopreserved‐thawed embryo transfer cycles with estrogen/progesterone replacement were divided randomly into two groups. Transdermal oestradiol was used in combination with vaginal progesterone suppositories for HR. The embryo transfer was performed on day 17 and/or day 20 of the HR therapy cycle in both groups. In Group A, 3000 IU of hCG was administered on days 17, 20, and 23. In Group B, hCG was not used.ResultsThere was no significant difference in the average age of the patients, the average number of previous assisted reproductive technology cycles, or the average number of embryo transfers between the two groups. The rates of pregnancy and implantation per embryo were 37.2% and 25.3%, respectively, in Group A and 35.6% and 21.7%, respectively, in Group B. The pregnancy and implantation rates were similar in both groups.ConclusionSupplementation with hCG is not beneficial for cryopreserved‐thawed embryo transfer in estrogen/progesterone replacement cycles.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Is human chorionic gonadotropin supplementation beneficial for frozen and thawed embryo transfer in estrogen/progesterone replacement cycles?: A randomized clinical trial

Shiotani

Matsumoto

Okamoto

et al. 2017

Reprod Medicine & Biology

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“…The reasoning for HCG priming was based on the fact that HCG/LH receptors are present in endometrium and even the expression of functional receptors appears to be cycledependent, being present from the proliferative phase and regulated by changes in the alternative splicing pattern [13]. Human chorionic gonadotropin, a major embryonic signal, plays a critical role in the initiation and maintenance of pregnancy To investigate possible direct effects of HCG on endometrial paracrine function in the human female in vivo, Licht and colleagues [12] developed an intrauterine microdialysis system that allows the continuous sampling from the uterine cavity over time as well as the application of exogenous HCG and the monitoring of the tissue response to this stimulus.…”

Section: Discussionmentioning

confidence: 99%

“Follicular HCG endometrium priming for IVF patients experiencing resisting thin endometrium. A proof of concept study”

Papanikolaou¹,

Kyrou²,

Zervakakou³

et al. 2013

J Assist Reprod Genet

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Purpose A thin endometrium is one of the most difficult problems encountered in assisted reproduction every day practice. Whether a daily dose of 150 IU HCG for 7 days concomitant with estrogen administration in estrogen replacement cycles can increase the endometrial thickness and improve pregnancy outcome, was the objective of the current study. Methods Seventeen infertile patients with successive implantation failures and resisting thin endometrium, being recipients of fresh donor or frozen embryos were recruited. This was a prospective cohort, proof of concept study, NCT01768247. On day-8 or 9 of the estrogen administration, and continuing 8 mg estrogen per day, subcutaneous injections of 150 IU HCG were initiated daily for 7 days. After a week on HCG priming, (day-14 or 15) endometrial thickness was controlled with ultrasound, and progesterone was initiated. Results Mean endometrial thickness was increased from 5.2 mm to 6 mm (p=0.008). 35.3 % of the patients had more than 20 % improvement of their endometrial thickness after HCG priming. 17 % achieved an endometrial thickness more than 7 mm, and 29.4 % did not improve their thickness at all. Interestingly, from the later two became pregnant. Overall, 41 % of them (7/17) finally delivered. Conclusions One hundred fifty IU HCG endometrial priming for 7 days in the proliferative phase of estrogen substituted cycles for frozen embryos is highly promising, as not only the thickness of the endometrium improves but also eventually the receptivity appears normalized.

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“…Various studies have been published concerning LHR splice variants, which play a significant role in LHR functions. In particular, alternative splicing appears to be associated with cycle-dependent regulation of LHR mRNA levels in human endometrium [21] and receptor down-regulation in rat corpora lutea [17]. In addition, the different types of splice variants have also been shown to take place in pathophysiological conditions, such as endometrial carcinomas, which is related to increased levels of spliced LHR transcripts [22] or in human ovarian [31,36] and breast epithelial cell tumors [15], in which alternative splicing is shown to be altered or ceased when compared to normal cells.…”

Section: Discussionmentioning

confidence: 99%

LH receptor gene expression in cumulus cells in women entering an ART program

Papamentzelopoulou

Mavrogianni

Partsinevelos

et al. 2012

J Assist Reprod Genet

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Purpose Luteinizing hormone (LH) exerts its actions through its receptor (LHR), which is mainly expressed in theca cells and to a lesser extent in oocytes, granulosa and cumulus cells. The aim of the present study was the investigation of a possible correlation between LHR gene and LHR splice variants expression in cumulus cells and ovarian response as well as ART outcome. Methods Forty patients undergoing ICSI treatment for male factor infertility underwent a long luteal GnRH-agonist downregulation protocol with a fixed 5-day rLH pretreatment prior to rFSH stimulation and samples of cumulus cells were collected on the day of egg collection. RNA extraction and cDNA preparation was followed by LHR gene expression investigation through real-time PCR. Furthermore, cumulus cells were investigated for the detection of LHR splice variants using reverse transcription PCR. Results Concerning LHR expression in cumulus cells, a statistically significant negative association was observed with the duration of ovarian stimulation (odds ratio00.23, p00.012). Interestingly, 6 over 7 women who fell pregnant expressed at least two specific types of LHR splice variants (735 bp, 621 bp), while only 1 out of 19 women that did not express any splice variant achieved a pregnancy. Conclusions Consequently, the present study provide a step towards a new role of LHR gene expression profiling as a biomarker in the prediction of ovarian response at least in terms of duration of stimulation and also a tentative role of LHR splice variants expression in the prediction of pregnancy success.

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Evidence for cycle-dependent expression of full-length human chorionic gonadotropin/luteinizing hormone receptor mRNA in human endometrium and decidua

Cited by 85 publications

References 27 publications

Is human chorionic gonadotropin supplementation beneficial for frozen and thawed embryo transfer in estrogen/progesterone replacement cycles?: A randomized clinical trial

Is human chorionic gonadotropin supplementation beneficial for frozen and thawed embryo transfer in estrogen/progesterone replacement cycles?: A randomized clinical trial

“Follicular HCG endometrium priming for IVF patients experiencing resisting thin endometrium. A proof of concept study”

LH receptor gene expression in cumulus cells in women entering an ART program

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