Evidence for fibroblasts as the major source of prostacyclin and prostaglandin synthesis in dental cyst in man

Harvey, W. Proctor; Guat-Chen, Foo; Gordon, Duncan A.; Meghji, S; Evans, A.F.; Harris, Malcolm

doi:10.1016/0003-9969(84)90059-1

Cited by 40 publications

(13 citation statements)

References 15 publications

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“…Because 3C8 Ag has turned out to be PGIS in the current study, fibroblast and FDC exhibit another common feature of expressing PGIS. Fibroblasts are the major producer of prostacyclin (40). When the requirement for FDC increases in the GC during immune response, fibroblasts in connective tissues may migrate to lymphoid follicles in response to certain chemokines and differentiate to FDCs as a result of interaction with GC B cells.…”

Section: Discussionmentioning

confidence: 99%

Human Follicular Dendritic Cells Express Prostacyclin Synthase: A Novel Mechanism to Control T Cell Numbers in the Germinal Center

Lee

Kim

et al. 2005

The Journal of Immunology

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Stromal cells in the lymphoid organs provide a microenvironment where lymphocytes undergo various biological processes such as development, homing, clonal expansion, and differentiation. Follicular dendritic cells (FDCs) in the primary and secondary follicles of the peripheral lymphoid tissues interact with lymphocytes by contacting directly or producing diffusible molecules. To understand the biological role of human FDC at the molecular level, we developed a mAb, 3C8, that recognizes FDC but not bone marrow-derived cells. Through expression cloning and proteome analysis, we identified the protein that is recognized by 3C8 mAb, which revealed that FDC expresses prostacyclin synthase. The 3C8 protein purified from FDC-like cells indeed displayed the enzymatic activity of prostacyclin synthase and converted PGH2 into prostacyclin. In addition, prostacyclin significantly inhibited proliferation of T cells but delayed their spontaneous apoptosis. These findings may help explain why T cells constitute only a minor population compared with B cells in the germinal center.

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Section: Discussionmentioning

confidence: 99%

Human Follicular Dendritic Cells Express Prostacyclin Synthase: A Novel Mechanism to Control T Cell Numbers in the Germinal Center

Lee

Kim

et al. 2005

The Journal of Immunology

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“…Their identity was confirmed by co-chromatography with authentic standards [26]. This was the first demonstration that dental cysts synthesised PGI2, and, in view of its bone resorbing activity demonstrated in vivo, indicated that in might play a role in the destruction of bone adjacent to the cyst wall.…”

Section: Resultsmentioning

confidence: 83%

“…Assays for PGE2 in tumour cell-treated fibroblast medium were performed using a commercial antiserum (Steranti). Prostaglandins from five apical cyst cultures were identified by thin-layer chromatography of immunoreactive material and comparison with known standards [26].…”

Section: Methodsmentioning

confidence: 99%

Prostaglandins in pathological bone resorption

Harvey

Meghji

1986

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“…Several bone-resorbing factors have been characterized, including prostaglandins (PGE 2 , PGI 2 ), leukotrienes, and collagenases that were already isolated from radicular cysts (Harvey et al, 1984;Meghji e t a l., 1989;Hoenig e t a l., 1991). However, the bone resorption process has been only partially inhibited by the action of cyclooxygenase and lipoxygenase inhibitors (Hoenig et al, 1991), indicating the participation of other mediators in this regulation.…”

Section: Discussionmentioning

confidence: 99%

Levels of GM-CSF, IL-3, and IL-6 in Fluid and Tissue from Human Radicular Cysts

Gervasio¹,

Silva²,

Taketomi³

et al. 2002

Journal of Dental Research

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Cytokines released by immune system cells play an important role in cyst enlargement. This study aimed to determine, by ELISA, the levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 3 (IL-3), and IL-6 in fluid and tissue from human radicular cysts. GM-CSF was found in 42.8% of the fluid samples (164.3 pg/mL) and IL-6 in 92.8% (641.4 pg/mL). No IL-3 was detected in any fluid samples. In the tissue samples, 28.6% were positive for IL-3 (369.2 pg/mL), 86.4% for IL-6 (92.4 pg/mL), and 95.8% for GM-CSF (200.5 pg/mL). It can be concluded that GM-CSF and IL-6 were widely found in the fluid and tissue samples. In contrast, IL-3 was found only in the cystic tissue, even though in few lesions. These cytokines may contribute to the inflammation, cystic growth, and bone resorption that characterize cystic lesions.

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Evidence for fibroblasts as the major source of prostacyclin and prostaglandin synthesis in dental cyst in man

Cited by 40 publications

References 15 publications

Human Follicular Dendritic Cells Express Prostacyclin Synthase: A Novel Mechanism to Control T Cell Numbers in the Germinal Center

Human Follicular Dendritic Cells Express Prostacyclin Synthase: A Novel Mechanism to Control T Cell Numbers in the Germinal Center

Prostaglandins in pathological bone resorption

Levels of GM-CSF, IL-3, and IL-6 in Fluid and Tissue from Human Radicular Cysts

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