Evidence for FSH-Dependent Upregulation of SPATA2 (Spermatogenesis-Associated Protein 2)

Onisto, Maurizio; Liliana, Slongo M.; Graziotto, Romina; Zotti, Lorenza; Negro, Alessandro; Merico, Maurizio; Moro, Enrico

doi:10.1006/bbrc.2001.4754

Cited by 15 publications

(3 citation statements)

References 19 publications

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“…Initially, SPATA2 was described as a protein that might be involved in spermatogenesis, as it is highly expressed in testis and strongly upregulated during spermatogenesis (Onisto et al., 2001). Interestingly, CYLD-deficient mice, despite having no overt phenotype, have been reported to show defects in spermatogenesis (Wright et al., 2007).…”

Section: Discussionmentioning

confidence: 99%

SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes

et al. 2016

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SummaryRecruitment of the deubiquitinase CYLD to signaling complexes is mediated by its interaction with HOIP, the catalytically active component of the linear ubiquitin chain assembly complex (LUBAC). Here, we identify SPATA2 as a constitutive direct binding partner of HOIP that bridges the interaction between CYLD and HOIP. SPATA2 recruitment to TNFR1- and NOD2-signaling complexes is dependent on HOIP, and loss of SPATA2 abolishes CYLD recruitment. Deficiency in SPATA2 exerts limited effects on gene activation pathways but diminishes necroptosis induced by tumor necrosis factor (TNF), resembling loss of CYLD. In summary, we describe SPATA2 as a previously unrecognized factor in LUBAC-dependent signaling pathways that serves as an adaptor between HOIP and CYLD, thereby enabling recruitment of CYLD to signaling complexes.

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Section: Discussionmentioning

confidence: 99%

SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes

et al. 2016

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“…SPR, upregulated in both males of P. sanguinolentus and C. feriatus, was also expressed in embryonic and larval stages of Drosophila as well as in the adult male nervous system, while the expression of SP was confined to the male reproductive system [62]. Maurizio et al [63] reported that SPATA2 was expressed in testis of rats and originated from Sertoli cells in the period of infantile-juvenile and increases gradually during growing up. Some genes like IR, SRY, and DMRT were specifically expressed in males.…”

Section: Genes Differentially Expressed In Females and Males Of P Sanguinolentusmentioning

confidence: 99%

Identification of Sex-Related Genes from the Three-Spot Swimming Crab Portunus sanguinolentus and Comparative Analysis with the Crucifix Crab Charybdis feriatus

Zhang

Waiho

Ikhwanuddin

et al. 2021

Animals

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Crabs within the family Portunidae are important marine species in both aquaculture and fishery sectors. The current aquaculture status of most portunids, however, still relies on wild-caught fisheries due to the lack of essential knowledge regarding their reproductive biology and underlying governing mechanism. With the advancement of sequencing technology, transcriptome sequencing has been progressively used to understand various physiological processes, especially on non-model organisms. In the present study, we compared the differentially expressed genes (DEGs) between sexes of Portunus sanguinolentus based on their gonadal transcriptome profiles and subsequently contrasted them with the gonadal DEGs of Charybdis feriatus, the other member of Family Portunidae. In total, 40,964 DEGs between ovaries and testes were uncovered, with 27,578 up- and 13,386 down-regulated in females. Among those, some sex-related DEGs were identified, including a dmrt-like (DMRT) gene which was specifically expressed in males. C. feriatus has approximately 63.5% of genes common with P. sanguinolentus, with 62.6% showing similar expression patterns. Interestingly, the DMRT gene was specifically expressed in male P. sanguinolentus while its homologous gene—doublesex (DSX)—was specifically expressed in male C. feriatus. The DEGs obtained from the gonadal transcriptome of P. sanguinolentus are a beneficial resource for future genetic and genomic research in P. sanguinolentus and its close species. The transcriptomic comparison analysis might provide references for better understanding the sex determination and differentiation mechanisms among portunids.

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“…Using a set of primers designed on the basis of human pd1 gene, the entire coding sequence was cloned from a rat testis cDNA pool and subsequently completed with 5’ and 3’ UTR regions [5]. Sequence analysis revealed an open reading frame encoding a protein of 511 amino acids with an overall identity of 85% between human and rat proteins.…”

Section: The Rat Orthologue Of Human Pd1: Spata2 (Spermatogenesis-assmentioning

confidence: 99%

The Story of SPATA2 (Spermatogenesis-Associated Protein 2): From Sertoli Cells to Pancreatic Beta-Cells

Maran

Tassone²,

Masola³

et al. 2009

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In an attempt to isolate new spermatogenesis-associated genes, pd1 was initially identified and cloned as a novel human cDNA sequence from testis cDNA library. The novel gene was submitted to GenBank under accession n° U28164 in 1996. PD1 expression was demonstrated at the Sertoli cell level with a production which appeared to be under the influence of neighbouring spermatogenic cells. The rat orthologue of human pd1 was further cloned and, according to the Gene Nomenclature Committee, was renamed spata2 (spermatogenesis-associated protein 2) gene on the basis of its FSH-dependent up-regulation and developmental expression. The analysis of the human and rat cDNA sequences disclosed an open reading frame for a protein of 520 and 511 amino acids respectively, with an overall identity of 85%. Subsequently, a zebrafish orthologue of the human spata2 gene was identified. The consensus open reading frame (1650 bp) encodes a polypeptide of 550 amino acids, which shares 37% identity with the human spata2. By means of whole-mount in situ hybridisation it has been shown that spata2 transcripts are maternally derived and become strongly localised in the central nervous system at early developmental stages. At the same time, RT-PCR analysis demonstrated that several adult zebrafish tissues expressed high level of spata2 mRNA providing evidence that this gene may have a broader function than previously described. More recently, novel findings have highlighted a potential role of spata2 during pancreatic development and β-cell proliferation. In this review we will discuss spata2 gene expression and regulation as well as focus on novel evidence, which suggests a role for this protein in pancreatic β-cell function.

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Evidence for FSH-Dependent Upregulation of SPATA2 (Spermatogenesis-Associated Protein 2)

Cited by 15 publications

References 19 publications

SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes

SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes

Identification of Sex-Related Genes from the Three-Spot Swimming Crab Portunus sanguinolentus and Comparative Analysis with the Crucifix Crab Charybdis feriatus

The Story of SPATA2 (Spermatogenesis-Associated Protein 2): From Sertoli Cells to Pancreatic Beta-Cells

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