Evidence for long‐term prevalence of cucumber vein yellowing virus in Sudan and genetic variation of the virus in Sudan and the Mediterranean Basin

Desbiez, Cécile; Caciagli, P.; Wipf-Scheibel, Catherine; Millot, Pauline; Ruiz, Leticia; Marian, Daniele; Dafalla, Gasim; Lecoq, Hervé

doi:10.1111/ppa.13055

Cited by 9 publications

(8 citation statements)

References 31 publications

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“…, the gene encoding a SARMP2-like protein) exists. From the point of view of resistance stability when confronted to different CVYV strains (Desbiez et al, 2019), the potential implication of RDR1a/b represents an optimistic perspective, given its broad spectrum of action (Leibman et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…In cucurbit-producing areas of heavy B. tabaci infestation, it can cause epidemics with massive yield losses and dramatic economic consequences. Although significant diversity has been reported for this virus, epidemics in Western Mediterranean countries seem to be associated to genetically uniform virus populations (Desbiez et al, 2019), perhaps as a consequence of single virus introductions followed by rapid epidemic expansions (Janssen et al, 2007). At the start of the CVYV epidemics, disease control relied heavily on early detection (Martínez-García et al, 2004) and eradication, and whitefly control.…”

Section: Introductionmentioning

confidence: 99%

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Mapping Cucumber Vein Yellowing Virus Resistance in Cucumber (Cucumis sativus L.) by Using BSA-seq Analysis

Pujol

Alexiou

Fontaine³

et al. 2019

Front. Plant Sci.

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Cucumber vein yellowing virus (CVYV) causes severe yield losses in cucurbit crops across Mediterranean countries. The control of this virus is based on cultural practices to prevent the presence of its vector (Bemisia tabaci) and breeding for natural resistance, which requires the identification of the loci involved and the development of molecular markers for linkage analysis. In this work, we mapped a monogenic locus for resistance to CVYV in cucumber by using a Bulked Segregant Analysis (BSA) strategy coupled with whole-genome resequencing. We phenotyped 135 F3 families from a segregating population between a susceptible pickling cucumber and a resistant Long Dutch type cucumber for CVYV resistance. Phenotypic analysis determined the monogenic and incomplete dominance inheritance of the resistance. We named the locus CsCvy-1. For mapping this locus, 15 resistant and 15 susceptible homozygous F2 individuals were selected for whole genome resequencing. By using a customized bioinformatics pipeline, we identified a unique region in chromosome 5 associated to resistance to CVYV, explaining more than 80% of the variability. The resequencing data provided us with additional SNP markers to decrease the interval of CsCvy-1 to 625 kb, containing 24 annotated genes. Markers flanking CsCvy-1 in a 5.3 cM interval were developed for marker-assisted selection (MAS) in breeding programs and will be useful for the identification of the target gene in future studies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mapping Cucumber Vein Yellowing Virus Resistance in Cucumber (Cucumis sativus L.) by Using BSA-seq Analysis

Pujol

Alexiou

Fontaine³

et al. 2019

Front. Plant Sci.

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“…Genes encoding the coat proteins (CP) of helical viruses were obtained from various sources: AltMV-CP (GenBank: FJ822136), SPFMV-CP (GenBank: NC001841) and PapMV-CP (GeneBank: D13957.1) were ordered from GeneArt (Thermo Fisher Scientific GENEART GmBH, Regensburg, Germany). CVYV-CP was amplified from cloned materials corresponding to the isolate CVYV-Esp (GeneBank: MK777994) [ 20 ] kindly provided by Cecile Desbiez (INRA, Avignon, France). TMV-CP/OAS was amplified from pEAQ- HT -TMV-CP/OAS [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants

Thuenemann

Byrne

Peyret

et al. 2021

Viruses

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The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies suggesting that an RNA scaffold may be necessary for their efficient production. To examine this, we compared the accumulation of VLPs from two potexviruses, papaya mosaic virus and alternanthera mosaic virus (AltMV), when the coat proteins were expressed from a replicating potato virus X- based vector (pEff) and a non-replicating vector (pEAQ-HT). Significantly greater quantities of VLPs could be purified when pEff was used. The pEff system was also very efficient at producing VLPs of helical viruses from different virus families. Examination of the RNA content of AltMV and tobacco mosaic virus VLPs produced from pEff revealed the presence of vector-derived RNA sequences, suggesting that the replicating RNA acts as a scaffold for VLP assembly. Cryo-EM analysis of the AltMV VLPs showed they had a structure very similar to that of authentic potexvirus particles. Thus, we conclude that vectors generating replicating forms of RNA, such as pEff, are very efficient for producing helical VLPs.

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“…Cucumber vein yellowing virus (CVYV) causes significant damage to cucumber and other cucurbit crops, such as zucchini and melon. The virus was first described in 1960 in Israel [1], and after its first finding it has now been reported in the Middle-East, the Mediterranean basin and Sudan [2][3][4][5][6][7][8][9][10]. Infected cucumber plants show netting and vein yellowing on young leaves, while older leaves show a general chlorosis [11].…”

Section: Introductionmentioning

confidence: 99%

The Amino-Proximal Region of the Coat Protein of Cucumber Vein Yellowing Virus (Family Potyviridae) Affects the Infection Process and Whitefly Transmission

Lindenau

Winter

Margaria

2021

Plants

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Most plant viruses rely on vector transmission for their spread and specific interactions between vector and virus have evolved to regulate this relationship. The whitefly Bemisia tabaci- transmitted cucumber vein yellowing virus (CVYV; genus Ipomovirus, family Potyviridae) is endemic in the Mediterranean Basin, where it causes significant losses in cucurbit crops. In this study, the role of the coat protein (CP) of CVYV for B. tabaci transmission and plant infection was investigated using a cloned and infectious CVYV cDNA and a collection of point and deletion mutants derived from this clone. Whitefly transmission of CVYV was abolished in a deletion mutant lacking amino acids in position 93–105 of the CP. This deletion mutant caused more severe disease symptoms compared to the cDNA clone representing the wild-type (wt) virus and movement efficiency was likewise affected. Two virus mutants carrying a partially restored CP were transmissible and showed symptoms comparable to the wt virus. Collectively, our data demonstrate that the N-terminus of the CVYV CP is a determinant for transmission by the whitefly vector and is involved in plant infection and symptom expression.

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Evidence for long‐term prevalence of cucumber vein yellowing virus in Sudan and genetic variation of the virus in Sudan and the Mediterranean Basin

Cited by 9 publications

References 31 publications

Mapping Cucumber Vein Yellowing Virus Resistance in Cucumber (Cucumis sativus L.) by Using BSA-seq Analysis

Mapping Cucumber Vein Yellowing Virus Resistance in Cucumber (Cucumis sativus L.) by Using BSA-seq Analysis

A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants

The Amino-Proximal Region of the Coat Protein of Cucumber Vein Yellowing Virus (Family Potyviridae) Affects the Infection Process and Whitefly Transmission

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