Evidence for multiple origins of identical insecticide resistance mutations in the aphid Myzus persicae

Anstead, James A.; Williamson, Martin S.; Denholm, I.

doi:10.1016/j.ibmb.2004.12.004

Cited by 65 publications

(59 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This indicates that populations were isolated from each other and kdr occurrence seems to be an independent effect probably reflecting the insecticide strategy used by each ranch. Multiple independent origins of kdr mutation appear to occur also in other organisms (ANSTEAD et al, 2005;ALON et al, 2006). In the same region, it is possible to find a population with a very low kdr allelic frequency and another one with relatively high frequency, as seen in the Central-West region; even in the same county (Corumbá, State of Mato Grosso do Sul, Central-West region), the percentage of kdr allelic frequency varied from 2 to 32.…”

Section: Discussionmentioning

confidence: 99%

Knockdown resistance in pyrethroid-resistant horn fly (Diptera: Muscidae) populations in Brazil

Sabatini¹,

Ribolla²,

Barros³

et al. 2009

RBPV

View full text Add to dashboard Cite

To investigate the kdr (knockdown resistance) resistance-associated gene mutation and determine its frequency in pyrethroid-resistant horn fly (Haematobia irritans) populations, a total of 1,804 horn flies of 37 different populations from all Brazilian regions (North, Northeast, Central-West, Southeast, and South) were molecular screened through polymerase chain reaction (PCR). The kdr gene was not detected in 87.08% of the flies. However, the gene was amplified in 12.92% of the flies, of which 11.70% were resistant heterozygous and 1.22% were resistant homozygous. Deviation from Hardy-Weinberg equilibrium (HWE) was found only in 1 ranch with an excess of heterozygous. When populations were grouped by region, three metapopulations showed significant deviations of HWE (Central-West population, South population and Southeast population). This indicates that populations are isolated one from another and kdr occurrence seems to be an independent effect probably reflecting the insecticide strategy used by each ranch. Although resistance to pyrethroids is disseminated throughout Brazil, only 48% of resistant populations had kdr flies, and the frequency of kdr individuals in each of these resistant populations was quite low. But this study shows that, with the apparent exception of the Northeast region, the kdr mechanism associated with pyrethroid resistance occurs all over Brazil.Keywords: Haematobia irritans, horn fly, resistance, pyrethroid. ResumoCom o objetivo de verificar a ocorrência e determinar a frequência da mutação kdr (knock down resistance) em populações de Haematobia irritans (mosca-dos-chifres) resistentes aos piretróides, foram analisados 1.804 indivíduos de 37 populações de todas as Regiões do Brasil. Com exceção da Região Nordeste, o kdr (knock down resistance gene) foi encontrado em populações de todas as regiões. A mutação não foi detectada em 87,08% dos indivíduos. Entretanto, o gene foi amplificado de 12,92% das moscas, das quais 11,70% se mostraram heterozigotas resistentes e 1,22% homozigotas resistentes. Em todas as populações verificou-se equilíbrio de acordo com a Lei de Hardy e Weinberg, exceto uma com excesso de heterozigotos. Entretanto, quando agrupamos diferentes populações numa metapopulação de acordo com a região geográfica, é possível observar um desvio nas populações Centro-Oeste, Sul e Sudeste, indicando isolamento populacional e que a ocorrência do kdr é provavelmente um efeito independente, talvez refletindo a estratégia de uso do inseticida de cada produtor. Apesar da resistência aos piretróides estar disseminada por todo o país, apenas 48% das populações resistentes apresentaram o kdr, e a frequência de indivíduos kdr nas populações resistentes se mostrou bastante baixa. À exceção da Região Nordeste, o mecanismo de resistência ligado ao kdr ocorre em todo o país.

show abstract

Section: Discussionmentioning

confidence: 99%

Knockdown resistance in pyrethroid-resistant horn fly (Diptera: Muscidae) populations in Brazil

Sabatini¹,

Ribolla²,

Barros³

et al. 2009

RBPV

View full text Add to dashboard Cite

show abstract

“…CYP6CM1 has, like other cases of resistance (Andreev et al, 1999;Ffrench-Constant et al, 2004;Anstead et al, 2005), multiple independent origins and has originated at least once in each biotype. It was reported previously that target-site resistance to pyrethroids evolved independently in the B and Q biotypes, most likely after pyrethroids were introduced to control B. tabaci in the field (Alon et al, 2006).…”

Section: Cyp6cm1vqmentioning

confidence: 99%

Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae)

Karunker

Benting

Lueke

et al. 2008

Insect Biochemistry and Molecular Biology

Self Cite

378

326

View full text Add to dashboard Cite

“…The first mutation in the sodium channel that conferred resistance was detected in Musca domestica. subsequently mutations in other insects have been identified including Culicidae resistant to pyrethroids (Martínez et al, 1998;Brengues et al, 2003;Anstead, 2005;Saavedra et al, 2007;Brooke, 2008;Chang et al 2009;Yanola et al, 2010).…”

Section: Voltage-gated Sodium Channelmentioning

confidence: 99%

Susceptibility Status of Aedes aegypti to Insecticides in Colombia

Serrano¹

2012

Insecticides - Pest Engineering

View full text Add to dashboard Cite

Evidence for multiple origins of identical insecticide resistance mutations in the aphid Myzus persicae

Cited by 65 publications

References 39 publications

Knockdown resistance in pyrethroid-resistant horn fly (Diptera: Muscidae) populations in Brazil

Knockdown resistance in pyrethroid-resistant horn fly (Diptera: Muscidae) populations in Brazil

Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae)

Susceptibility Status of Aedes aegypti to Insecticides in Colombia

Contact Info

Product

Resources

About