Evidence for natural transformation of Bacillus subtilis in foodstuffs

Bräutigam, Martin; Hertel, Christian; Hammes, Walter P.

doi:10.1111/j.1574-6968.1997.tb12691.x

Cited by 11 publications

(3 citation statements)

References 11 publications

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“…In the future more environmental compartments where gene transfer occurs will be identified. It is conceivable that Bräutigam et al, 1997;Davison, 1999;Day, 1998;Kay et al, 2002;Li et al, 2001;Lorenz and Wackernagel, 1994;Mercer et al, 2001;Schlimme et al, 1997;Stotzky et al, 1990. with improved experimental techniques gene transfer will be confirmed in very specific habitats where detection efforts have so far failed. For instance, it may be possible to identify natural transformation in the gastrointestinal tract, a habitat known to be rich in DNA degrading activity and probably not optimal for the development of DNA uptake competence of bacteria, but where high concentrations of bacteria are exposed to a variety of DNA molecules ingested with the food (Jonas et al, 2001).…”

Section: Microbial Horizontal Gene Transfer Processesmentioning

confidence: 95%

Microbial horizontal gene transfer and the DNA release from transgenic crop plants

Vries

Wackernagel

2005

Plant Soil

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Intraspecific and interspecific horizontal gene transfers among prokaryotes by mechanisms like conjugation, transduction and transformation are part of their life style. Experimental data and nucleotide sequence analyses show that these processes appear to occur in any prokaryotic habitat and have shaped microbial genomes throughout evolution over hundreds of million years. Here we summarize studies with a focus on the possibility of the transfer of free recombinant DNA released from transgenic plants to microorganisms by transformation. A list of 87 species capable of natural transformation is presented. We discuss monitoring techniques which allowed detection of the spread of intact DNA from plants during their growth, in the process of decay and by pollen dispersal including novel biomonitoring assays for measuring the transforming potential of DNA in the environment. Also, studies on the persistence of free DNA in soil habitats and the potential of bacteria to take up DNA in soil are summarized. On the other hand, the various barriers evolved in prokaryotes which suppress interspecific gene transfer and recombination will be addressed along with studies aiming to estimate the chance of a gene transfer from plant to microbe. The results suggest that, although such transfers could be possible in principle, each of the many steps involved from the release of intact DNA from a plant cell to integration into a prokaryotic genome has such a low probability that a successful transfer event be extremely rare. Further, interspecies transfer of chromosomal DNA is mostly negative for the recipient, and, if not, in the absence of a selective advantage the transformant will be lost. It is stressed that the nucleotide sequences introduced into transgenic plants are much less likely to be captured from the transgenic plants than directly from those organisms (often bacteria or viruses) from which they were originally derived.

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Section: Microbial Horizontal Gene Transfer Processesmentioning

confidence: 95%

Microbial horizontal gene transfer and the DNA release from transgenic crop plants

Vries

Wackernagel

2005

Plant Soil

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“…These results, and those obtained in challenge experiments performed to investigate the effect of added DNase I in sausages, indicate that the meat matrix had a protective effect on free plasmid DNA. Further information of DNA stability in milk was reported by Bräutigam et al [24], who showed that free bacterial DNA remained detectable for 12 days in ultra high temperature-treated milk stored at 4 °C whereas storage at 20 °C resulted in a rapid degradation within a few days.…”

Section: Dna Stabilitymentioning

confidence: 84%

Methods for Detection of Genetically Modified Microorganisms used in Food Fermentation Processes

Hammes

Hertel

Bauer

2006

Genetically Engineered Food

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“…Thus, environmental determinants are important factors affecting natural transformation. Natural transformation has been observed and thoroughly studied in a wide variety of bacterial species, including Streptococcus pneumoniae (S. pneumoniae) (Lefrancois et al, 1998;Johnsborg and Havarstein, 2009;Straume et al, 2015), Neisseria (Facius and Meyer, 1993;Hamilton and Dillard, 2006;Zhang et al, 2013), Bacillus subtilis (B. subtilis) (Kosovich and Prozorov, 1990;Brautigam et al, 1997;Le et al, 2017), Haemophilus influenzae (H. influenzae) (Redfield, 1993;Mell et al, 2011), Vibrio cholerae (V. cholerae) (Frischer et al, 1990;Watve et al, 2014;Dalia et al, 2017), and Acinetobacter baylyi (A. baylyi) (Vaneechoutte et al, 2006;Merod and Wuertz, 2014;Utnes et al, 2015;Hulter et al, 2017;Leong et al, 2017;Suarez et al, 2017;Nero et al, 2018;Mantilla-Calderon et al, 2019). Although most bacteria possess competence genes, numerous conditions or signals trigger competence and are often species specific (Seitz and Blokesch, 2013).…”

Section: Introductionmentioning

confidence: 99%

Effect of Nutritional Determinants and TonB on the Natural Transformation of Riemerella anatipestifer

Zhang

Huang

et al. 2021

Front. Microbiol.

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Riemerella anatipestifer is a gram-negative bacterium that is the first naturally competent bacterium identified in the family Flavobacteriaceae. However, the determinants that influence the natural transformation and the underlying mechanism remain unknown. In this study, we evaluated the effects of various nutritional factors of the GCB medium [glucose, L-glutamine, vitamin B1, Fe (NO3)3, NaCl, phosphate, and peptone], on the natural transformation of R. anatipestifer ATCC 11845. Among the assayed nutrients, peptone and phosphate affected the natural transformation of R. anatipestifer ATCC 11845, and the transformation frequency was significantly decreased when phosphate or peptone was removed from the GCB medium. When the iron chelator 2,2′-dipyridyl (Dip) was added, the transformation frequency was decreased by approximately 100-fold and restored gradually when Fe (NO3)3 was added, suggesting that the natural transformation of R. anatipestifer ATCC 11845 requires iron. Given the importance of TonB in nutrient transportation, we further identified whether TonB is involved in the natural transformation of R. anatipestifer ATCC 11845. Mutation of tonBA or tonBB, but not tbfA, was shown to inhibit the natural transformation of R. anatipestifer ATCC 11845 in the GCB medium. In parallel, it was shown that the tonBB mutant, but not the tonBA mutant, decreased iron acquisition in the GCB medium. This result suggested that the tonBB mutant affects the natural transformation frequency due to the deficiency of iron utilization.

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Evidence for natural transformation of Bacillus subtilis in foodstuffs

Cited by 11 publications

References 11 publications

Microbial horizontal gene transfer and the DNA release from transgenic crop plants

Microbial horizontal gene transfer and the DNA release from transgenic crop plants

Methods for Detection of Genetically Modified Microorganisms used in Food Fermentation Processes

Effect of Nutritional Determinants and TonB on the Natural Transformation of Riemerella anatipestifer

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