Evidence for Non-adrenergic Non-cholinergic Contractile Responses in Bovine and Swine Trachea

Matera, M; Amorena, Michele; Marabese, Ida; Loffreda, A.; D.»Agostino, B; Lucisano, A.; Rossi, Francesco

doi:10.1006/pupt.1997.0081

Cited by 8 publications

(5 citation statements)

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“…The muscle contractions directly elicited by SP and NKA are suggested to be predominantly mediated by the activation of NK1-R, which is in line with previous reports [13,19]. NK receptor antagonists were applied to further support this suggestion since muscle contractile responses and Ca 2+ mobilization evoked by SP and NKA were fully abolished by the potent and selective NK1-R antagonist, L732138 [20], but not by the selective NK2-R and NK3-R antagonists [21,22].…”

Section: Discussionsupporting

confidence: 68%

Involvement of Ca2+ signaling in tachykinin-mediated contractile responses in swine trachea

2005

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Neuropeptide tachykinins, present within sensory nerves, have been implicated as neurotransmitters involved in nonadrenergic and noncholinergic airway muscle contraction. The signal transduction pathways of tachykinins on muscle contraction and Ca2+ mobilization were investigated in swine trachea. Tachykinins, substance P (SP) and neurokinin A (NKA), concentration (1 nM to 1 microM)-dependently induced contractile responses with removal of epithelium, whereas neurokinin B (NKB) did not alter the muscle tension. The SP- and NKA-evoked muscle contractions were inhibited by NK1-R antagonist L732138, but not by either NK2-R antagonist MDL29913 or NK3-R antagonist SB218795. Consistently, SP-elicited increase in [Ca2+]i was abolished by NK1-R antagonist, neither by NK2-R nor NK3-R antagonists. The SP-induced muscular responses were significantly inhibited by L-type Ca2+ channel blocker verapamil and withdrawal of external Ca2+. Caffeine (10 mM) or ryanodine (50 microM) also partly suppressed the SP-induced muscle responses. Inhibition of inositol 1,4,5-trisphosphate (InsP3) receptor with 2-APB (75 microM) potently attenuated SP-evoked Ca2+ mobilization and muscle contraction, which was further inhibited by 2-APB under Ca2+-free external solution, but not completely. Unexpectedly, simultaneous blockade of InsP3 receptor and ryanodine receptor (RyR) by 2-APB and ryanodine enhanced SP-evoked muscle contraction and Ca2+ mobilization. This potentiation was virtually abolished by removal of external Ca2+, suggesting native Ca2+ channels may contribute to this phenomenon. These results demonstrate that tachykinins produce a potent muscle contraction associated with Ca2+ mobilization via tachykinin NK1- R-dependent activation of multiple signal transduction pathways involving Ca2+ influx and release of Ca2+ from InsP3- and ryanodine-sensitive Ca2+ stores. Blockade of both InsP3 receptor and RyR enhances the Ca2+ influx through native Ca2+ channels in plasma membrane, which is crucial to Ca2+ signaling in response to NK1 receptor activation.

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Section: Discussionsupporting

confidence: 68%

Involvement of Ca2+ signaling in tachykinin-mediated contractile responses in swine trachea

2005

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“…Since HTS has been proposed to stimulate C-fibers 29 , which also respond to capsaicin, we tested whether capsaicin (10 µM) affected HTS treatment 30 . Agarose beads loaded with capsaicin (ITS + Cap) resulted in an increase in the ASL layer height greater than in swine treated with ITS (ITS) nebulization alone (Fig.…”

Section: Resultsmentioning

confidence: 99%

Nebulized hypertonic saline triggers nervous system-mediated active liquid secretion in cystic fibrosis swine trachea

Luan

Tam

Belev

et al. 2019

Sci Rep

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Inhaled hypertonic saline (HTS) treatment is used to improve lung health in patients with cystic fibrosis (CF). The current consensus is that the treatment generates an osmotic gradient that draws water into the airways and increases airway surface liquid (ASL) volume. However, there is evidence that HTS may also stimulate active secretion of ASL by airway epithelia through the activation of sensory neurons. We tested the contribution of the nervous system and airway epithelia on HTS-stimulated ASL height increase in CF and wild-type swine airway. We used synchrotron-based imaging to investigate whether airway neurons and epithelia are involved in HTS treatment-triggered ASL secretion in CFTR−/− and wild-type swine. We showed that blocking parasympathetic and sensory neurons in airway resulted in ~50% reduction of the effect of HTS treatment on ASL volume in vivo. Incubating tracheal preparations with inhibitors of epithelial ion transport across airway decreased secretory responses to HTS treatment. CFTR−/− swine ex-vivo tracheal preparations showed substantially decreased secretory response to HTS treatment after blockage of neuronal activity. Our results indicated that HTS-triggered ASL secretion is partially mediated by the stimulation of airway neurons and the subsequent activation of active epithelia secretion; osmosis accounts for only ~50% of the effect.

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“…SP‐immunoreactive fibers emerging from the vagus nerve, along with sympathetic nerve fibers, are also present within the trachea wall (Baluk & Gabella, 1989). During experiments using tracheal smooth muscle this neuronal component is usually left intact, and its functionality is easily disclosed by electrical field stimulation (Baker et al ., 1993; Matera et al ., 1997; Sommer et al ., 1997). On the other hand, it is now well documented that caffeine induces release of brain neurotransmitters such as serotonin, dopamine and noradrenaline (Nehlig et al ., 1992).…”

Section: Discussionmentioning

confidence: 99%

Acetylcholine and tachykinins involvement in the caffeine‐induced biphasic change in intracellular Ca²⁺ in bovine airway smooth muscle

Montaño

Carbajal

Arreola

et al. 2003

British J Pharmacology

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1 Caffeine has been widely used as a pharmacological tool to evaluate Ca 2 þ release from the sarcoplasmic reticulum in isolated smooth muscle cells. However, in nervous tissue this drug also causes neurotransmitters release, which might cause additional effects when smooth muscle strips are evaluated. To assess this last possibility, simultaneous measurements of contraction and cytosolic Ca 2 þ concentration (using Fura -2/AM) were carried out in bovine airway smooth muscle strips during caffeine stimulation. 2 A first stimulation (S1, n ¼ 11) with caffeine (10 mm) induced a biphasic change in cytosolic Ca 2 þ , which consisted of a transient Ca 2 þ peak (254740 nm, X X7SEM) followed by a plateau (92713 nm), and a transient contraction (204.72731.56 mg tension mg tissue À1 ). A second caffeine stimulation (S2) produced a similar response but these parameters had a different magnitude. The S2/S1 ratios for these parameters were 0.6970.02, 0.8370.06 and 1.0170.03, respectively. Addition of o-conotoxin GVIA (1 mm) and tetrodotoxin (3.1 mm) before S2 significantly diminished these S2/S1 ratios (0.2670.05, 0.2670.09 and 0.6470.11, respectively, n ¼ 5, Po0.05), implicating the neurotransmitters release involvement in the response to caffeine. A similar effect (Po0.01) was observed with atropine (1 mm, n ¼ 4), the fragment 4 -11 of substance P (SP) (an SP receptor antagonist, 10 mm, n ¼ 5), and with both substances (n ¼ 4). 3 We discarded a direct effect of o-conotoxin GVIA (1 mm) plus tetrodotoxin (3.1 mm) or of atropine (1 mm) plus SP fragment 4 -11 on smooth muscle cells because they did not modify caffeine responses in isolated tracheal myocytes. 4 We confirmed by HPLC that caffeine increased the release of acetylcholine (from 0.4370.19 to 2.0770.56 nm mg tissue À1 , Po0.02) in bovine airway smooth muscle strips. Detection of substance P by ELISA was not statistically different after caffeine stimulation (geometric means before and after caffeine, 0.69 vs. 1.97 pg ml À1 mg tissue À1 , respectively, P ¼ 0.053). 5 We concluded that acetylcholine and tachykinins release are involved in the caffeine-induced biphasic changes in cytosolic Ca 2 þ concentration.

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Evidence for Non-adrenergic Non-cholinergic Contractile Responses in Bovine and Swine Trachea

Cited by 8 publications

References 1 publication

Involvement of Ca2+ signaling in tachykinin-mediated contractile responses in swine trachea

Involvement of Ca2+ signaling in tachykinin-mediated contractile responses in swine trachea

Nebulized hypertonic saline triggers nervous system-mediated active liquid secretion in cystic fibrosis swine trachea

Acetylcholine and tachykinins involvement in the caffeine‐induced biphasic change in intracellular Ca²⁺ in bovine airway smooth muscle

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Evidence for Non-adrenergic Non-cholinergic Contractile Responses in Bovine and Swine Trachea

Cited by 8 publications

References 1 publication

Involvement of Ca2+ signaling in tachykinin-mediated contractile responses in swine trachea

Involvement of Ca2+ signaling in tachykinin-mediated contractile responses in swine trachea

Nebulized hypertonic saline triggers nervous system-mediated active liquid secretion in cystic fibrosis swine trachea

Acetylcholine and tachykinins involvement in the caffeine‐induced biphasic change in intracellular Ca2+ in bovine airway smooth muscle

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Acetylcholine and tachykinins involvement in the caffeine‐induced biphasic change in intracellular Ca²⁺ in bovine airway smooth muscle