Evidence for paternal DNA transmission to gynogenetic grass carp

Mao, Zhuangwen; Fu, Yeqing; Wang, Yude; Wang, Shi; Zhang, Minghe; Gao, Xin; Luo, Kun; Qin, Qinbo; Chun, Zhang; Tao, Min; Yao, Zhanzhou; Liu, Shaojun

doi:10.1186/s12863-018-0712-x

Cited by 19 publications

(3 citation statements)

References 12 publications

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“…In studies of androgenetic rainbow trout, maternal chromosomal fragments were found in karyotyped-treated offspring (Ocalewicz et al, 2004). Paternally derived markers were found in treated offspring of gynogenetic grass carp (Ctenopharyngodon idellus) using microsatellites (Mao et al, 2019), and in gynogenetic gibel carp (Carassius gibelio)…”

Section: Discussionmentioning

confidence: 99%

“…In studies of androgenetic rainbow trout, maternal chromosomal fragments were found in karyotyped‐treated offspring (Ocalewicz et al ., 2004). Paternally derived markers were found in treated offspring of gynogenetic grass carp ( Ctenopharyngodon idellus ) using microsatellites (Mao et al ., 2019), and in gynogenetic gibel carp ( Carassius gibelio ) using PCR primers specific to the paternal donor and subsequent amplicon sequencing (Chen et al ., 2020; Yi et al ., 2003). The diversity of findings of these studies highlights a benefit of using genome‐wide approaches that enable assignment of alleles and haplotypes to the parents, particularly the male, as the authors have done here.…”

Section: Discussionmentioning

confidence: 99%

“…Although some studies have shown that the transmission of genomic fragments is detrimental (Dorafshan et al ., 2014; Michalik et al ., 2015; Ocalewicz et al ., 2010), other researchers have taken advantage of the presence of paternally derived residuals (Chen et al ., 2020; Thorgaard et al ., 1985). Although the degree of chromosomal fragmentation can vary by the intensity of irradiation (Chen et al ., 2020; Chourrout & Quillet, 1982; Komen & Thorgaard, 2007; Mao et al ., 2019; Thorgaard et al ., 1985; Yi et al ., 2003), the precise magnitude and frequency of genetic leakage from sires into gynogenetic diploids are largely unknown. Developing efficient computational genomic methodologies to quantify the genome‐wide paternal contribution is essential to research using gynogenetic fish.…”

Section: Introductionmentioning

confidence: 99%

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Genome‐wide analysis facilitates estimation of the amount of male contribution in meiotic gynogenetic three‐spined stickleback (Gasterosteus aculeatus)

Currey

Walker

Bassham

et al. 2023

Journal of Fish Biology

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Gynogenetic embryosthose inheriting only maternal DNAcan be experimentally created by fertilizing eggs with radiation-treated sperm containing inactivated paternal chromosomes. Diploidy in the zygotes can be maintained through prevention of the second meiosis or restored by preventing the first mitosis after the maternal chromosome complement has been replicated. These gynogenetic organisms are useful in many fields including aquaculture, evolutionary biology and genomics. Although gynogenetic organisms have been created in numerous species, the completeness of uni-parental inheritance has often been assumed rather than thoroughly quantified across the genome. Instead, when tests of uni-parental inheritance occur, they typically rely on well-studied genetically determined phenotypes that represent a very small sub-set of the genome. Only assessing small genomic regions for paternal inheritance leaves the question of whether some paternal contributions to offspring might still have occurred. In this study, the authors quantify the efficacy of creating gynogenetic diploid three-spined stickleback fish (Gasterosteus aculeatus). To this end, the authors mirrored previous assessments of paternal contribution using well-studied genetically determined phenotypes including sex and genetically dominant morphological traits but expanded on previous studies using dense restriction site-associated DNA sequencing (RAD-seq) markers in parents and offspring to assess paternal inheritance genome-wide. In the gynogenetic diploids, the authors found no male genotypes underlying their phenotypes of interestsex and dominant phenotypic traits. Using genome-wide assessments of paternal contribution, nevertheless, the authors found evidence of a small, yet potentially important, amount of paternally "leaked" genetic material. The application of this genome-wide approach identifies the need for more widespread assessment of paternal contributions to gynogenetic animals and promises benefits for many aspects of aquaculture, evolutionary biology and genomics.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome‐wide analysis facilitates estimation of the amount of male contribution in meiotic gynogenetic three‐spined stickleback (Gasterosteus aculeatus)

Currey

Walker

Bassham

et al. 2023

Journal of Fish Biology

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Comparative analysis and gut bacterial community assemblages of grass carp and crucian carp in new lineages from the Dongting Lake area

et al. 2020

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Gut microbiota are known to play an important role in health and nutrition of the host and have been attracting an increasing attention. Farming of new lineages of grass carp and crucian carp has been developed rapidly as these species were found to outperform indigenous ones in terms of growth rate and susceptibility to diseases. Despite this rapid development, no studies have addressed the characteristics of their gut microbiota as a potential factor responsible for the improved characteristics. To reveal whether microbiomes of the new lineages are different from indigenous ones, and therefore could be responsible for improved growth features, intestinal microbiota from the new lineages were subjected to high‐throughput sequencing. While the phyla Firmicutes, Fusobacteria and Proteobacteria were representing the core bacterial communities that comprised more than 75% in all fish intestinal samples, significant differences were found in the microbial community composition of the new linages versus indigenous fish populations, suggesting the possibility that results in the advantages of enhanced disease resistance and rapid growth for the new fish lineages. Bacterial composition was similar between herbivorous and omnivorous fish. The relative abundance of Bacteroidetes and Actinobacteria was significantly higher in omnivores compared to that of herbivores, whereas Cetobacterium_sp. was abundant in herbivores. We also found that the gut microbiota of freshwater fish in the Dongting lake area was distinct from those of other areas. Network graphs showed the reduced overall connectivity of gut bacteria in indigenous fish, whereas the bacteria of the new fish lineage groups showed hubs with more node degree. A phylogenetic investigation of communities by reconstruction of unobserved states inferred function profile showed several metabolic processes were more active in the new lineages compared to indigenous fish. Our findings suggest that differences in gut bacterial community composition may be an important factor contributing to the rapid growth and high disease resistance of the new fish lineages.

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Rapid Genomic and Epigenetic Alterations in Gynogenetic Carassius auratus Red Var. Derived from Distant Hybridization

et al. 2020

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Evidence for paternal DNA transmission to gynogenetic grass carp

Cited by 19 publications

References 12 publications

Genome‐wide analysis facilitates estimation of the amount of male contribution in meiotic gynogenetic three‐spined stickleback (Gasterosteus aculeatus)

Genome‐wide analysis facilitates estimation of the amount of male contribution in meiotic gynogenetic three‐spined stickleback (Gasterosteus aculeatus)

Comparative analysis and gut bacterial community assemblages of grass carp and crucian carp in new lineages from the Dongting Lake area

Rapid Genomic and Epigenetic Alterations in Gynogenetic Carassius auratus Red Var. Derived from Distant Hybridization

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