Evidence for Recombination of Mitochondrial DNA in Triploid Crucian Carp

Guo, Xing; Liu, Shao Jun; Liu, Yun

doi:10.1534/genetics.105.049841

Cited by 71 publications

(54 citation statements)

References 39 publications

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“…However, some females and males of F 2 hybrids were able to generate diploid eggs and diploid sperm, respectively, which fertilized each other to form the bisexual tetraploid hybrids in F 3 . Until now the population of F 3 -F 16 was proved to be tetraploid hybrids, indicating that their tetraploidy could be stably inherited from one generation to another (Liu et al 2001(Liu et al , 2004Sun et al 2003;Guo et al 2006). In that study, the formation of unreduced diploid gametes was due to the premeiotic endoreduplication of oogonia or spermatogonia of females or males of the F 2 (Liu et al 2001).…”

Section: Discussionmentioning

confidence: 96%

The Formation of the Polyploid Hybrids From Different Subfamily Fish Crossings and Its Evolutionary Significance

et al. 2007

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This study provides genetic evidences at the chromosome, DNA content, DNA fragment and sequence, and morphological levels to support the successful establishment of the polyploid hybrids of red crucian carp 3 blunt snout bream, which belonged to a different subfamily of fish (Cyprininae subfamily and Cultrinae subfamily) in the catalog. We successfully obtained the sterile triploid hybrids and bisexual fertile tetraploid hybrids of red crucian carp (RCC) ($) 3 blunt snout bream (BSB) (#) as well as their pentaploid hybrids. The triploid hybrids possessed 124 chromosomes with two sets from RCC and one set from BSB; the tetraploid hybrids had 148 chromosomes with two sets from RCC and two sets from BSB. The females of tetraploid hybrids produced unreduced tetraploid eggs that were fertilized with the haploid sperm of BSB to generate pentaploid hybrids with 172 chromosomes with three sets from BSB and two sets from RCC. The ploidy levels of triploid, tetraploid, and pentaploid hybrids were confirmed by counting chromosomal number, forming chromosomal karyotype, and measuring DNA content and erythrocyte nuclear volume. The similar and different DNA fragments were PCR amplified and sequenced in triploid, tetraploid hybrids, and their parents, indicating their molecular genetic relationship and genetic markers. In addition, this study also presents results about the phenotypes and feeding habits of polyploid hybrids and discusses the formation mechanism of the polyploid hybrids. It is the first report on the formation of the triploid, tetraploid, and pentaploid hybrids by crossing parents with a different chromosome number in vertebrates. The formation of the polyploid hybrids is potentially interesting in both evolution and fish genetic breeding.

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Section: Discussionmentioning

confidence: 96%

The Formation of the Polyploid Hybrids From Different Subfamily Fish Crossings and Its Evolutionary Significance

et al. 2007

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“…In the present context, the question is whether Muller's ratchet contributes to mutation accumulation in mitochondrial genomes, so that estimates of recombination should use whole-genome sequence data (e.g. Gantenbein et al 2005;Guo et al 2006;Ujvari et al 2007).…”

Section: How Much Recombination Does It Take To Counter Mutation Accumentioning

confidence: 99%

The causes of mutation accumulation in mitochondrial genomes

2009

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A fundamental observation across eukaryotic taxa is that mitochondrial genomes have a higher load of deleterious mutations than nuclear genomes. Identifying the evolutionary forces that drive this difference is important to understanding the rates and patterns of sequence evolution, the efficacy of natural selection, the maintenance of sex and recombination and the mechanisms underlying human ageing and many diseases. Recent studies have implicated the presumed asexuality of mitochondrial genomes as responsible for their high mutational load. We review the current body of knowledge on mitochondrial mutation accumulation and recombination, and conclude that asexuality, per se, may not be the primary determinant of the high mutation load in mitochondrial DNA (mtDNA). Very little recombination is required to counter mutation accumulation, and recent evidence suggests that mitochondrial genomes do experience occasional recombination. Instead, a high rate of accumulation of mildly deleterious mutations in mtDNA may result from the small effective population size associated with effectively haploid inheritance. This type of transmission is nearly ubiquitous among mitochondrial genomes. We also describe an experimental framework using variation in mating system between closely related species to disentangle the root causes of mutation accumulation in mitochondrial genomes.

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“…Both events have been documented to occur in mammals (Gyllensten et al, 1991;Schwartz and Vissing, 2002;Ladoukakis and Eyre-Walker, 2004;Zhao et al, 2004), birds (Kvist et al, 2003), fish (Magoulas and Zouros, 1993;Guo et al, 2006;Ciborowski et al, 2007), mollusks (Ladoukakis and Zouros, 2001), amphibians (Ujvari et al, 2007), arthropods (Meusel and Moritz, 1993;Gantenbein et al, 2005;Arunkumar et al, 2006;Sherengul et al, 2006;Fontaine et al, 2007) and nematodes (Lunt and Hyman, 1997;Armstrong et al, 2007). What is more, gender-associated and tissue-specific transmission of both paternal and maternal mtDNA ('doubly uniparental inheritance' (DUI)) appears to be the norm in some bivalves (Breton et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Lost in the zygote: the dilution of paternal mtDNA upon fertilization

Wolff¹,

Gemmell²

2008

Heredity

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The mechanisms by which paternal inheritance of mitochondrial DNA (mtDNA) (paternal leakage) and, subsequently, recombination of mtDNA are prevented vary in a speciesspecific manner with one mechanism in common: paternally derived mtDNA is assumed to be vastly outnumbered by maternal mtDNA in the zygote. To date, this dilution effect has only been described for two mammalian species, human and mouse. Here, we estimate the mtDNA content of chinook salmon oocytes to evaluate the dilution effect operating in another vertebrate; the first such study outside a mammalian system. Employing real-time PCR, we determined the mtDNA content of chinook salmon oocytes to be 3.2 Â 10 9 ± 1.0 Â 10 9 , and recently, we determined the mtDNA content of chinook salmon sperm to be 5.73 ± 2.28 per gamete. Accordingly, the ratio of paternal-to-maternal mtDNA if paternal leakage occurs is estimated to be 1:5.5 Â 10 8 . This contribution of paternal mtDNA to the overall mtDNA pool in salmon zygotes is three to five orders of magnitude smaller than those revealed for the mammalian system, strongly suggesting that paternal inheritance of mtDNA per offspring will be much less likely in this system than in mammals.

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Evidence for Recombination of Mitochondrial DNA in Triploid Crucian Carp

Cited by 71 publications

References 39 publications

The Formation of the Polyploid Hybrids From Different Subfamily Fish Crossings and Its Evolutionary Significance

The Formation of the Polyploid Hybrids From Different Subfamily Fish Crossings and Its Evolutionary Significance

The causes of mutation accumulation in mitochondrial genomes

Lost in the zygote: the dilution of paternal mtDNA upon fertilization

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