2013
DOI: 10.1007/s12015-013-9472-1
|View full text |Cite
|
Sign up to set email alerts
|

Evidence for self-maintaining pluripotent murine stem cells in embryoid bodies

Abstract: Pluripotent stem cells have great potential for regenerative medicine; however, their clinical use is associated with a risk of tumor formation. We utilized pluripotent cells expressing green fluorescent protein and puromycin resistance under control of the Oct4 promoter to study the persistence of potential pluripotent cells under embryoid body (EB) culture conditions, which are commonly used to obtain organotypic cells. We found that i.) OCT4-expressing cells dramatically decrease during the first week of di… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
8
0

Year Published

2014
2014
2020
2020

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 10 publications
(8 citation statements)
references
References 37 publications
0
8
0
Order By: Relevance
“…Moreover, the LIF effect is lost on cells differentiated for two days or more, stressing the point that LIF displays differential effects depending upon cell maturity and that there is a decisive step for commitment between two and three days of LIF withdrawal. Furthermore, EBs grown for more than 20 days in vitro can re-express Oct4 and recover pluripotent properties, presenting another window of potential plasticity in the mESC model [ 72 ]. However, it has not been described whether this late effect is LIF-dependent, which could be a possibility since LIF is expressed in differentiated cells from day 6 and later ([ 42 ] and our unpublished results).…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, the LIF effect is lost on cells differentiated for two days or more, stressing the point that LIF displays differential effects depending upon cell maturity and that there is a decisive step for commitment between two and three days of LIF withdrawal. Furthermore, EBs grown for more than 20 days in vitro can re-express Oct4 and recover pluripotent properties, presenting another window of potential plasticity in the mESC model [ 72 ]. However, it has not been described whether this late effect is LIF-dependent, which could be a possibility since LIF is expressed in differentiated cells from day 6 and later ([ 42 ] and our unpublished results).…”
Section: Discussionmentioning
confidence: 99%
“…Intensive studies revealed that transcriptional factors, such as Snail, Slug, and Twist regulate the EMT process [24]. Slug, the best-characterized member of the Snail superfamily, regulate chondrogenic phenotypes in MSCs [25]. In lung cancer cells, co-overexpression of Oct4 and Nanog enhanced the tumor-initiating capability through activation of Slug expression [26].…”
Section: Discussionmentioning
confidence: 99%
“…Mechanisms behind the DNA repair switch that occurs upon reprogramming to pluripotency and how reprogramming can revert DNA repair functionalities are still unknown. Utilizing systems biology-based technologies to profile the reprogramming factors’ interactome could provide answers [ 23 ].…”
Section: Dna Damage and Repair Status During Reprogrammingmentioning
confidence: 99%