Evidence for sibling species in Cryptocercus punctulatus, the wood roach, from variation in mitochondrial DNA and karyotype

Fossil calibrations for the cockroach phylogeny (Insecta, Dictyoptera, Blattodea), comments on the use of wings for their identification, and a redescription of the oldest Bla

“…Molecular phylogenetic studies of cockroaches go back more than 20 years (Kambhampati et al, 1996), but molecular dating of cockroach phylogenies has only begun recently (Djernaes et al, 2015;Legendre et al, 2015;Che et al, 2017). One of the crucial points in divergence time estimation is choosing the correct fossils for age calibration, as these are highly influential on posterior age estimates (e.g., Inoue et al, 2010).…”

Coevolution between a cockroach and its bacterial endosymbiont: a biogeographical perspective

“…Cryptocercus darwini, C. garciai, C. punctulatus and C. wrighti were collected in the Appalachian mountains in the eastern USA (for details, see Kambhampati et al 1996;Burnside et al 1999). Cryptocercus clevelandi were collected during August 1999 in Oregon.…”

“…Primers for the ampli¢cation of the host mitochondrial 16S rRNA and 12S rRNA genes were given in Kambhampati et al (1996). Primers for the ampli¢cation of the endosymbiont genes (table 1) were designed, based on sequences from GenBank.…”

“…Two species of Cryptocercus occur in the palaearctic, one each in eastern Russia (Cryptocercus relictus) and south-central China (Cryptocercus primarius). Recent work has established the existence of ¢ve species in the nearctic (Kambhampati et al 1996;Nalepa et al 1997;Burnside et al 1999). One species, Cryptocercus clevelandi, is found in the northwestern USA while the other four, Cryptocercus darwini, Cryptocercus garciai, Cryptocercus punctulatus and Cryptocercus wrighti, are found in the southeastern USA.…”

What does Cryptocercus kyebangensis, n.sp. (Dictyoptera: Blattaria: Polyphagidae) from Korea reveal about Cryptocercus evolution? A study in morphology, molecular phylogeny, and chemistry of tergal glands

“…Because species in the genus Cryptocercus show slight interspecific variations in structure, researchers mainly use sequence divergence in the mitochondrial DNA (Nalepa et al 1997;Burnside et al 1999;Grandcolas et al 2001;Grandcolas et al 2005) in addition to some aspects of female genitalia (Grandcolas 2000;Aldrich et al 2004;Grandcolas et al 2005) and chromosome number (Kambhampati et al 1996;Burnside et al 1999) or bacterial endosymbionts (Clark et al 2003) to distinguish species. Aldrich et al (2004) indicated that the female genitalia exhibit consistent, species-specific variation and can be used to identify each of the 4 Cryptocercus species that occur in the Appalachian Mountains.…”