Evidence for tandem integration of avian myeloblastosis virus DNA with endogenous provirus in leukemic chicken cells.

Shoyab, Mohammed; Thorley‐Lawson, David A.; Baluda, M A

doi:10.1073/pnas.73.5.1749

Cited by 16 publications

(11 citation statements)

References 35 publications

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“…Such repetitious DNA sequences have been implicated in gene regulation and chromosome structure (15). Also, proviral DNA sequences in normal and transformed chicken cells have been shown to be integrated next to middle repetitious cellular DNA sequences which appear to be nonrandomly interspersed among nonrepetitive sequences in eukaryotes (16,19,20,23,29).…”

mentioning

confidence: 99%

Homogeneity and complexity of avian oncornavirus proviral DNA determined by molecular hybridization

Evans¹,

Shoyab²,

Drohan³

et al. 1977

J Virol

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The homogeneity of DNA complementary to the 35S RNA subunit of avian myeloblastosis virus (AMV) has been demonstrated by single or multistep hybridization. For multistep hybridizations, 35S AMV RNA was preselected for its ability to hybridize either to unfractionated leukemic DNA or to leukemic DNA enriched for unique or for reiterated sequences. These experiments indicate that the viral genome is complementary to DNA sequences with a low reiteration frequency. Competition experiments confirm the absence of fasthybridizing sequences in viral DNA. Computer analyses of the data reveal that there are two to four copies of viral DNA in infected cells.

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mentioning

confidence: 99%

Homogeneity and complexity of avian oncornavirus proviral DNA determined by molecular hybridization

Evans¹,

Shoyab²,

Drohan³

et al. 1977

J Virol

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“…Other authors have shown that certain viruses such as SV40 [31] and polyoma [32] may be integrated in tan dem in the DNA of chronically infected cells, and Shoyab el at. [33] provided evidence that myeloblastosis virus is integrated in tandem with chicken endogenous virus. Tandem trans fections observed here are best explained by the integration of the two parental proviruses into the same integration site or two adjacent or slightly separate sites on the chromosomal DNA.…”

Section: Discussionmentioning

confidence: 99%

Tandem Integration of Avian Retroviruses in Double-Infected Cells

Carloni¹,

N²,

Hillova³

et al. 1980

Intervirology

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Chicken embryo fibroblasts were chronically infected by both RAV-1 and SR-E, and established SR-E-transformed quail cells were superinfected with RAV-1. About 75 and 50% of double-infected and superinfected cells, respectively, produced both parental viruses. The DNA purified from these cells contained infectious provirus of both parents and no detectable recombinant provirus. In assays using agar overlay, about 5% of the transformed foci that were induced by the DNA of double-infected, but not superinfected, cells were found to contain the progeny of both parents. This observation suggests that in double infections with avian retroviruses, DNA of both parent viruses may integrate into the host genome either in tandem or in close proximity.

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“…Melting profiles of the hybrids strongly suggest the presence of heterogeneous closely related sequences [6]. Whether these multiple endogenous viral genomes are localized randomly or in tandem [10] is the next question to be approached.…”

Section: Fig 1 a Hybridization Kinetics O F T-felv 3h-cdna With Framentioning

confidence: 99%

Reiteration Frequency of Feline Type C Viral Genomes in Homologous and Heterologous Host Cell DNA

Okabe¹,

duBuy²,

Hatanaka³

et al. 1978

Intervirology

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Differences in the multiplicity of proviral sequences of two feline endogenous viruses, RD-114 and FeLV, in homologous and heterologous host DNA were examined by viral cDNA hybridization using cellular DNA fractionated with respect to reiteration frequency. The endogenous proviral DNAs in cat cells were fractionated predominantly with intermediate-repeated sequences, while RD-114 proviralDNA in virus-infected human cells was fractionated together with unique-sequence DNA. These differences were consistent with the results of kinetic analysis using unfractionated DNA.

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Evidence for tandem integration of avian myeloblastosis virus DNA with endogenous provirus in leukemic chicken cells.

Cited by 16 publications

References 35 publications

Homogeneity and complexity of avian oncornavirus proviral DNA determined by molecular hybridization

Homogeneity and complexity of avian oncornavirus proviral DNA determined by molecular hybridization

Tandem Integration of Avian Retroviruses in Double-Infected Cells

Reiteration Frequency of Feline Type C Viral Genomes in Homologous and Heterologous Host Cell DNA

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