Evidence for the existence of cAMP-dependent protein kinase phosphorylation system associated with specific phosphoproteins in stable microtubules from rat cerebral cortex

Development of polarity in human erythroleukemia cells: Roles of membrane ruffling and the centrosome

“…It has been reported that a cAMP-dependent protein kinase is associated with a partly purified preparation of cold-stable microtubules from rat cerebral cortex [Perez et al, 1993]. Other in vitro effects of cyclic AMP that could affect microtubules within cells have been reported: cyclic AMP stimulates phosphorylation of tau [Johnson, 1992], and phosphoryla- tion of a polypeptide in Paramecium that copurifies with the 22S dynein arm, thus increasing the velocity with which the 22S dynein causes microtubule gliding [Hamasaki et al, 1991].…”

Chronic Antidepressant Treatment Prevents Accumulation of Gsα in Cholesterol-Rich, Cytoskeletal-Associated, Plasma Membrane Domains (Lipid Rafts)

“…Chronic desipramine treatment has also been reported to phosphorylate certain microtubule-associated proteins (Perez et al, 1989). A fraction of PKA II associates with microtubules (Perez et al, 1993;Theurkauf and Vallee, 1982) via MAP2, which serves as an anchor and substrate for PKA II (Obar et al, 1989;Rubino et al, 1989). Thus, chronic PKA II activation as seen during antidepressant treatment leads to MAP2 phosphorylation and a subsequent decrease in microtubule assembly (Perez et al, 2000).…”

Abnormalities of cAMP signaling in affective disorders: implications for pathophysiology and treatment

“…An overall assessment of such investigations reveals that almost all intracellular pathways can regulate or be regulated by PKA [31–33]. Yet, another way to accomplish PKA modulation is by localizing the enzyme to specific cellular compartments [31,35,36]. Indeed, compartmentalization of the PKA seems to represent a regulatory mechanism that may increase the selectivity and intensity of cAMP‐mediated biological responses.…”

“…Indeed, compartmentalization of the PKA seems to represent a regulatory mechanism that may increase the selectivity and intensity of cAMP‐mediated biological responses. In this regard, it has been reported that up to 75% of the type‐II PKA is associated through an interaction between the RII subunit, with anchoring proteins to a wide array of subcellular structures, including centrosomes, endoplasmic reticulum, Golgi complex, microtubules, mitochondria, nuclear matrix, and secretory granules, thereby regulating phosphorylation processes in specific cell compartments [31,35,36].…”