Evidence for the existence of an estrogen-responsive sexually dimorphic group of cells in the medial preoptic area of the 129SvEv mouse strain

Hill, Rachel Anne; Simpson, Evan R.; Boon, Wah Chin

doi:10.1038/ijir.2008.2

Cited by 8 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More than one article has described the lack of preoptic sexual dimorphism in one of the most widely used mouse strains, C57BL/6J (Hill et al, 2008), by Nissl staining or by using galanin or aromatase immunoreactivity (Brown et al, 1999;Hill et al, 2008). Unlike in other species, sexual dimorphic structures in the mice, when present, were in some cases larger in females than in males, as in the Nissl-stained anteroventral periventricular nucleus in the present study.…”

Section: Sexual Dimorphism In the Mouse Preoptic Areacontrasting

confidence: 56%

“…The cluster of galanin-immunoreactive neurons in the 129SvEv mouse POA also does not depend on androgen. Aromatase gene knockout (KO) mice with the 129SvEv background, which have higher titers of testosterone, exhibit a feminine size of this structure (Hill et al, 2008). It may be that the reported cluster in the POA is different from the aggregate of CB neurons in the present study, because the galanin-immunoreactive cluster lacks a sex difference in C57BL/6J mice and cannot be visualized by Nissl staining (Hill et al, 2008).…”

Section: Lack Of Effect Of Dhtmentioning

confidence: 74%

See 1 more Smart Citation

Estrogen configures sexual dimorphism in the preoptic area of C57BL/6J and ddN strains of mice

Orikasa

Sakuma

2010

J of Comparative Neurology

View full text Add to dashboard Cite

Immunohistochemistry using a calbindin D28k antibody revealed a marked sex difference in neuronal distribution in the central portion of the medial preoptic area in C57BL/6J and ddN strains of mice when the animals were sacrificed on D65 (D1 = the day of birth). Male mice had a distinct ellipsoidal cell aggregate, whereas females lacked such a structure. This sex difference was not observed in Nissl-stained sections. Co-localization of calbindin D28k and the neuron-specific nuclear protein NeuN confrmed that the cells in the aggregate were neurons. The aggregates were larger in males than in females in both strains. When observed on D65, males orchidectomized on D1 had smaller aggregates. However, daily injections of 2 microg estradiol benzoate through D1-D5 as well as a single injection of 100 microg testosterone propionate on D1 enlarged the aggregates in females, but a single injection of 100 microg dihydrotestosterone on D1 had no effect on the female phenotype. Similar endocrine manipulations had no effects in adult animals of both sexes. Thus, the calbindin-immunoreactive cell aggregates in the preoptic area of C57BL/6J and ddN mice are homologous to the sexually dimorphic nucleus of the rat preoptic area in terms of the morphology and sex steroid-dependent organization.

show abstract

Section: Sexual Dimorphism In the Mouse Preoptic Areacontrasting

confidence: 56%

Section: Lack Of Effect Of Dhtmentioning

confidence: 74%

Estrogen configures sexual dimorphism in the preoptic area of C57BL/6J and ddN strains of mice

Orikasa

Sakuma

2010

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…Aromatase knockout (ArKO) female mice are infertile as their reproductive organs do not develop properly (Simpson, 2004). These mice show impaired functionality in the amygdala and hypothalamus (Pierman et al, 2008) and increased apoptosis in the frontal cortex (Hill et al, 2008). Various studies on ArKO mice have implicated estrogen in neurodevelopment (Balthazart et al, 1991;Sasahara et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Ketamine attenuates cytochrome p450 aromatase gene expression and estradiol‐17β levels in zebrafish early life stages

Trickler

Guo

Cuevas

et al. 2013

J of Applied Toxicology

View full text Add to dashboard Cite

Ketamine, a dissociative anesthetic, is a noncompetitive antagonist of N-methyl-D-aspartate-type glutamate receptors. In rodents and non-human primates as well as in zebrafish embryos, ketamine has been shown to be neurotoxic. In cyclic female rats, ketamine has been shown to decrease serum estradiol-17β(E2) levels. E2 plays critical roles in neurodevelopment and neuroprotection. Cytochrome p450 (CYP) aromatase catalyzes E2 synthesis from androgens. Although ketamine down-regulates a number of CYP enzymes in rodents, its effect on the CYP aromatase (CYP19) is not known. Zebrafish have been used as a model system for examining mechanisms underlying drug effects. Here, using wild-type (WT) zebrafish (Danio rerio) embryos, we demonstrate that ketamine significantly reduced E2 levels compared with the control. However, the testosterone level was elevated in ketamine-treated embryos. These results are concordant with data from mammalian studies. Ketamine also attenuated the expression of the ovary form of CYP aromatase (cyp19a1a) at the transcriptional level but not the brain form of aromatase, cyp19a1b. Exogenous E2 potently induced the expression of cyp19a1b and vtg 1, both validated biomarkers of estrogenicity and endocrine disruption, but not cyp19a1a expression. Attenuation of activated ERK/MAPK levels, reportedly responsible for reduced human cyp19 transcription, was also observed in ketamine-treated embryos. These results suggest that reduced E2 levels in ketaminetreated embryos may have resulted from the suppression of cyp19a1a transcription. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.

show abstract

“…Morale et al ( 42 ) suggested that the inhibition of cytochrome p450 aromatase function contributes to Parkinson's disease. Others have demonstrated that female mice are infertile when the aromatase gene is silenced, as their reproductive organs cannot develop properly ( 43 ), that the function of the amygdala and hypothalamus in these mice is impaired ( 44 ) and that apoptosis in the frontal cortex is increased ( 45 ). Numerous studies on aromatase knockout mice have suggested that estrogen is important for neurodevelopment ( 46 ).…”

Section: Discussionmentioning

confidence: 99%

Selective regulation of neurosteroid biosynthesis under ketamine-induced apoptosis of cortical neurons in vitro

Wang

et al. 2015

Molecular Medicine Reports

View full text Add to dashboard Cite

Numerous studies have suggested that ketamine administration can induce neuroapoptosis in primary cultured cortical neurons. Neurosteroids modulate neuronal function and serve important roles in the central nervous system, however the role of neurosteroids in neuroapoptosis induced by ketamine remains to be elucidated. The present study aimed to explore whether neurosteroidogenesis was a pivotal mechanism for neuroprotection against ketamine-induced neuroapoptosis, and whether it may be selectively regulated under ketamine-induced neuroapoptosis conditions in primary cultured cortical neurons. To study this hypothesis, the effect of ketamine exposure on neurosteroidogenesis in primary cultured cortical neurons was investigated. Cholesterol, a substrate involved in the synthesis of neurosteroids, was added to the culture medium, and neurosteroids were quantified using high-performance liquid chromatography-tandem mass spectrometry analysis. The data demonstrated that cholesterol blocked ketamine-induced neuroapoptosis by promoting the synthesis of various neurosteroids, and the pathway of neurosteroid testosterone conversion into estradiol was inhibited by ketamine exposure. These data suggest that endogenous neurosteroids biosynthesis is critical for neuroprotection against ketamine-induced neuroapoptosis and inhibiting the biosynthesis of neuroprotective-neurosteroid estradiol is of notable importance for ketamine-induced neuroapoptosis.

show abstract

Evidence for the existence of an estrogen-responsive sexually dimorphic group of cells in the medial preoptic area of the 129SvEv mouse strain

Cited by 8 publications

References 36 publications

Estrogen configures sexual dimorphism in the preoptic area of C57BL/6J and ddN strains of mice

Estrogen configures sexual dimorphism in the preoptic area of C57BL/6J and ddN strains of mice

Ketamine attenuates cytochrome p450 aromatase gene expression and estradiol‐17β levels in zebrafish early life stages

Selective regulation of neurosteroid biosynthesis under ketamine-induced apoptosis of cortical neurons in vitro

Contact Info

Product

Resources

About