McKay et al. [(1996)Science 273, 924 -930] suggested that carbonate globules in the meteorite ALH84001 contained the fossil remains of Martian microbes. We have characterized a subpopulation of magnetite (Fe3O4) crystals present in abundance within the Fe-rich rims of these carbonate globules. We find these Martian magnetites to be both chemically and physically identical to terrestrial, biogenically precipitated, intracellular magnetites produced by magnetotactic bacteria strain MV-1. Specifically, both magnetite populations are single-domain and chemically pure, and exhibit a unique crystal habit we describe as truncated hexaoctahedral. There are no known reports of inorganic processes to explain the observation of truncated hexa-octahedral magnetites in a terrestrial sample. In bacteria strain MV-1 their presence is therefore likely a product of Natural Selection. Unless there is an unknown and unexplained inorganic process on Mars that is conspicuously absent on the Earth and forms truncated hexaoctahedral magnetites, we suggest that these magnetite crystals in the Martian meteorite ALH84001 were likely produced by a biogenic process. As such, these crystals are interpreted as Martian magnetofossils and constitute evidence of the oldest life yet found. W e report here the presence of single-domain, chemically pure, truncated hexa-octahedral magnetite crystals in terrestrial samples and Martian meteorite ALH84001. We suggest that the truncated hexa-octahedral magnetite crystals in the Martian meteorite ALH84001 were likely formed by a biogenic process. These magnetite crystals are embedded in Ϸ3.91 (Ϯ0.05)-Ga-old carbonate globules (1) that fill cracks and pore space in the 4.5-Ga-old Martian meteorite ALH84001. These truncated hexa-octahedral magnetite crystals are identical to those produced intracellularly by the marine magnetotactic bacterium strain MV-1 (2-7); natural selection has optimized the magnetic moment of MV-1 magnetite particles (2,(8)(9)(10)(11)(12). There is no known natural terrestrial inorganic mechanism that can explain the observation of truncated hexa-octahedral magnetite crystals associated with MV-1. Therefore, unless there is an unknown inorganic process on Mars, which seems to be absent on the Earth, we suggest that ALH84001 truncated hexaoctahedral magnetites formed by a similar mechanism to their terrestrial biogenic counterparts. As such, these crystals are interpreted as Martian magnetofossils and they constitute evidence of the oldest life yet found. In support of this, we note that early Mars likely had free-standing bodies of liquid water (13,14), and both organic (15) and inorganic carbon and energy (e.g., atmospheric CO 2 13 ) sources. Furthermore, early Mars also likely possessed a substantial planetary magnetic field (16), which would have been sufficient to support the evolution and growth of magnetotactic bacteria.
Characteristics of Biogenic MagnetiteMagnetotactic bacteria produce well ordered membranebounded intracellular crystals of magnetite (Fe 3 O 4 ) and͞or greigi...