Evidence for the involvement of sperm angiotensin converting enzyme in fertilization

Foresta, Carlo; Mioni, Roberto; Rossato, Marco; Varotto, A.; Zorzi, Marco

doi:10.1111/j.1365-2605.1991.tb01101.x

Cited by 49 publications

(40 citation statements)

References 21 publications

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“…A role of the tACE in capacitation has been suggested by several authors (211,658). Previous reports have suggested that ACE is released during sperm capacitation (356), which was confirmed after exposure to ACE inhibition that affected the acrosome reaction and the ability of human sperm to penetrate an egg (212).…”

Section: Male Reproductive Tractmentioning

confidence: 86%

Physiology of Local Renin-Angiotensin Systems

Paul

Mehr²,

Kreutz³

2006

Physiological Reviews

1,512

1,371

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Since the first identification of renin by Tigerstedt and Bergmann in 1898, the renin-angiotensin system (RAS) has been extensively studied. The current view of the system is characterized by an increased complexity, as evidenced by the discovery of new functional components and pathways of the RAS. In recent years, the pathophysiological implications of the system have been the main focus of attention, and inhibitors of the RAS such as angiotensin-converting enzyme (ACE) inhibitors and angiotensin (ANG) II receptor blockers have become important clinical tools in the treatment of cardiovascular and renal diseases such as hypertension, heart failure, and diabetic nephropathy. Nevertheless, the tissue RAS also plays an important role in mediating diverse physiological functions. These focus not only on the classical actions of ANG on the cardiovascular system, namely, the maintenance of cardiovascular homeostasis, but also on other functions. Recently, the research efforts studying these noncardiovascular effects of the RAS have intensified, and a large body of data are now available to support the existence of numerous organ-based RAS exerting diverse physiological effects. ANG II has direct effects at the cellular level and can influence, for example, cell growth and differentiation, but also may play a role as a mediator of apoptosis. These universal paracrine and autocrine actions may be important in many organ systems and can mediate important physiological stimuli. Transgenic overexpression and knock-out strategies of RAS genes in animals have also shown a central functional role of the RAS in prenatal development. Taken together, these findings may become increasingly important in the study of organ physiology but also for a fresh look at the implications of these findings for organ pathophysiology.

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Section: Male Reproductive Tractmentioning

confidence: 86%

Physiology of Local Renin-Angiotensin Systems

Paul

Mehr²,

Kreutz³

2006

Physiological Reviews

1,512

1,371

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“…A previous study has shown that spontaneous capacitation, acrosomal reaction, and premature capacitation of frozen-thawed spermatozoa are inhibited by ACE inhibitors (Foresta et al 1991), which indicates that ACE may also be an important factor for premature capacitation in frozen-thawed sperm. The levels of ACE on the surface of spermatozoa have been shown to be inversely proportional to their fertilization capacity (Kohn et al 1998, Shibahara et al 2001.…”

Section: Proteins Related To Premature Capacitationmentioning

confidence: 94%

Identification of differentially expressed proteins in fresh and frozen–thawed boar spermatozoa by iTRAQ-coupled 2D LC–MS/MS

Chen

Zhu²,

Hu³

et al. 2014

Reproduction

101

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Cryodamage is a major problem in semen cryopreservation, causing changes in the levels of proteins that influence the function and motility of spermatozoa. In this study, protein samples prepared from fresh and frozen-thawed boar spermatozoa were compared using the isobaric tags for relative and absolute quantification (iTRAQ) labeling technique coupled to 2D LC-MS/MS analysis. A total of 41 differentially expressed proteins were identified and quantified, including 35 proteins that were present at higher levels and six proteins that were present at lower levels in frozen-thawed spermatozoa by at least a mean of 1.79-fold (P!0.05). On classifying into ten distinct categories using bioinformatic analysis, most of the 41 differentially expressed proteins were found to be closely relevant to sperm premature capacitation, adhesions, energy supply, and sperm-oocyte binding and fusion. The expression of four of these proteins, SOD1, TPI1, ODF2, and AKAP3, was verified by western blot analysis. We propose that alterations in these identified proteins affect the quality of cryopreserved semen and ultimately lower its fertilizing capacity. This is the first study to compare protein levels in fresh and frozenthawed spermatozoa using the iTRAQ technology. Our preliminary results provide an overview of the molecular mechanisms of cryodamage in frozen-thawed spermatozoa and theoretical guidance to improve the cryopreservation of boar semen.

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“…Angiotensin II is not the only RAS component ascribed with functionality in the testicular RAS. Recent studies have established that Ang (1-7) activation of the Mas receptor is necessary for spermatogenesis in mice and rats [37] and that testicular ACE is necessary for capacitation and subsequent fertilization [38][39][40][41][42]. Thus, this novel Ang II/Ang III binding site could participate in Ang II-regulated male reproductive functions.…”

Section: Tissue Distributionmentioning

confidence: 99%

Distribution of a novel binding site for angiotensins II and III in mouse tissues

Rabey

Karamyan

Speth

2010

Regulatory Peptides

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A novel binding site for angiotensins II and III that is unmasked by parachloromercuribenzoate has been reported in rat, mouse and human brains. Initial studies of this binding site indicate that it is not expressed in the adrenal, liver or kidney of the rat and mouse. To determine if this binding site occurs in other mouse tissues, 8 tissues were assayed for expression of this binding site by radioligand binding assay and compared with the expression of this binding site in the forebrain. Particulate fractions of homogenates of testis, epididymis, seminal vesicles, heart, spleen, pancreas, lung, skeletal muscle, and forebrain were incubated with (125)I-sarcosine(1), isoleucine(8) angiotensin II in the presence or absence of 0.3mM parachloromercuribenzoate plus 10microM losartan and 10microM PD123319 (to saturate AT(1) and AT(2) receptors). Specific (3microM angiotensin II displaceable) high affinity binding occurred in the testis>forebrain>epididymis>spleen>pancreas>lung when parachloromercuribenzoate was present. Binding could not be reliably observed in heart, skeletal muscle and seminal vesicles. High affinity binding of (125)I-sarcosine(1), isoleucine(8) angiotensin II was observed in the absence of parachloromercuribenzoate in the pancreas on occasion. This suggests that this novel angiotensin binding site may have a functional role in these tissues.

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Evidence for the involvement of sperm angiotensin converting enzyme in fertilization

Cited by 49 publications

References 21 publications

Physiology of Local Renin-Angiotensin Systems

Physiology of Local Renin-Angiotensin Systems

Identification of differentially expressed proteins in fresh and frozen–thawed boar spermatozoa by iTRAQ-coupled 2D LC–MS/MS

Distribution of a novel binding site for angiotensins II and III in mouse tissues

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