Evidence for the local production and utilization of immune reactants in rheumatoid arthritis

Cecere, Fred; Lessard, James; McDuffy, S; Pope, Richard M.

doi:10.1002/art.1780251106

Cited by 32 publications

(4 citation statements)

References 16 publications

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“…The 1gM:HSA and 1gG:HSA ratios obtained are shown in Table 3. The relative Ig concentrations in rheumatoid synovial fluids approached the values found in sera in previous tests by other investigators (17). Comparison of the ratios measured in rheumatoid cartilage extracts indicated that there was an 11-20-fold relative enrichment of Ig with respect to synovial fluids.…”

Section: Resultssupporting

confidence: 77%

Autoantibody specificities of immune complexes sequestered in articular cartilage of patients with rheumatoid arthritis and osteoarthritis

Jasin

1985

Arthritis & Rheumatism

194

114

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To define autoantibody specificities of immune complexes sequestered in articular cartilage of patients with rheumatoid arthritis and osteoarthritis, extracts were obtained from articular cartilage specimens from 16 patients with rheumatoid arthritis, 11 patients with osteoarthritis, and 6 normal controls. Radioimmunoassays of the extracts revealed that rheumatoid cartilage contained 37 times more IgM and 14 times more IgG than did normal cartilage extracts. In addition, osteoarthritic cartilage contained 3 times more IgM and IgG than the normal tissues. IgM rheumatoid factor was found in 13 of 16 rheumatoid cartilage extracts but in none of 11 osteoarthritic or 6 normal control extracts. IgG rheumatoid factor was detected in 4 of 7 seropositive rheumatoid but in none of 5 osteoarthritic cartilage extracts. More than 60% of the rheumatoid cartilage extracts were positive for native and denatured collagen I1 antibodies. Surprisingly, 50% of the osteoarthritic specimens also contained significant titers of collagen antibodies. Similar results were obtained with osteoarthritic menisci extracts. These findings indicate that the immune complexes sequestered in rheumatoid cartilage contain autoantibodies that are probably synthesized locally by cells infiltrating the inflamed synovium. If immune complexes trapped in cartilage play an important role in cartilage damage, our findings would provide a possible pathogenic mechanism that explains the self-perpetuating and chronic nature of cartilage degradation in rheumatoid arthritis and osteoarthritis.The pathophysiologic mechanisms operative in articular cartilage damage in rheumatoid arthritis (RA) are not completely understood. However, the pathologic changes observed in advanced disease indicate that most of the cartilage erosive lesions are associated with the invasion of the articular and deep areas of cartilage by pannus originating from the inflamed synovium (1,2). Although the conditions that initiate and facilitate pannus invasion are unknown, previous work in our laboratory suggested that sequestered immune complexes commonly found in the superficial areas of rheumatoid cartilage (3) may play a role in the destructive process by inducing pannus invasion (4) or by activation of the resident mononuclear phagocytes within this granulation tissue (5,6). Rheumatoid synovial fluids frequently contain large amounts of immunoglobulin with autoantibody specificities, synthesized in part by plasma cells infiltrating the subsynovial membrane (7,8). Thus, it was of particular interest to define the possible autoantibody specificities of immune complexes sequestered in articular cartilage. Our findings indicate that rheumatoid factor (RF) and/or collagen antibodies are found in the majority of cartilage tissue specimens obtained from joints of patients with advanced RA. Surprisingly, more than 50% of specimens from patients with osteoarthritis (OA) also contained collagen antibodies. These findings may provide an explanation for the self-perpetuating and chronic nature of ...

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Section: Resultssupporting

confidence: 77%

Autoantibody specificities of immune complexes sequestered in articular cartilage of patients with rheumatoid arthritis and osteoarthritis

Jasin

1985

Arthritis & Rheumatism

194

114

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“…A model of simple diffusion across the SM has been established by measuring nonimmunoglobulin molecules, which are synthesized in the liver, in paired serum and S F samples (35)(36)(37)(38)(39). A linear relationship (diffusion line) has been shown between the SF: serum concentration ratios of these proteins and the log of their molecular weights (38). In essence, this is a measure of SM permeability, which will vary with the degree of inflammation present.…”

Section: Patientsmentioning

confidence: 99%

Effects of gold therapy on the synthesis and quantity of serum and synovial fluid IgM, IgG, and IgA rheumatoid factors in rheumatoid arthritis patients

Hanly

Hassan

Whelan

et al. 1986

Arthritis & Rheumatism

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Eleven patients with active rheumatoid arthritis were monitored prospectively while receiving up to 1 gm of gold sodium thiomalate. There was a significant decrease in serum and synovial fluid IgG, IgA, and IgM rheumatoid factor (RF) levels over the period of study. Comparison of changes in serum RF and total immunoglobulin levels indicated a selective effect on RF production. These observations were supported by changes in the spontaneous in vitro production of IgM-RF and total IgM by peripheral blood mononuclear cells. Studies of synovial membrane synthesis showed a downward trend in immunoglobulin and RF production, but this did not reach statistical significance. A differential effect on the various RF classes was also noted. The most profound effect was on IgM-RF production; whereas, changes in IgG-RF production were least affected. These results suggest a selective and differential effect of gold salts on RF production.High levels of serum IgM rheumatoid factor (RF) in patients with rheumatoid arthritis (RA) are generally associated with more severe articular disease and a higher prevalence of extraarticular manifestations ( 1 4 ) . However, there is no consistent cor-

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“…Die phagozytierten Substanzen werden vorwiegend in der Synovialis produziert (12,13,14). Im Gegensatz zu Untersuchungen an fixierten Zellen gibt es nur wenige Mitteilungen über den Vorgang der Phagozytose, in denen vitale Zellen der Synovialflüssigkeit in Suspension mit Hilfe von Immunfluoreszenz untersucht wurden.…”

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C3c-Complement- und immunglobulinpositive Leukozyten in der Synovialflüssigkeit von chronischen Polyarthritiden, Arthritiden unterschiedlicher Genese, Arthrosen, traumatisch induzierten sowie postoperativen Ergüssen

Maerker-Alzer¹,

Dill²,

Dienst³

1988

Akt Rheumatol

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Evidence for the local production and utilization of immune reactants in rheumatoid arthritis

Cited by 32 publications

References 16 publications

Autoantibody specificities of immune complexes sequestered in articular cartilage of patients with rheumatoid arthritis and osteoarthritis

Autoantibody specificities of immune complexes sequestered in articular cartilage of patients with rheumatoid arthritis and osteoarthritis

Effects of gold therapy on the synthesis and quantity of serum and synovial fluid IgM, IgG, and IgA rheumatoid factors in rheumatoid arthritis patients

C3c-Complement- und immunglobulinpositive Leukozyten in der Synovialflüssigkeit von chronischen Polyarthritiden, Arthritiden unterschiedlicher Genese, Arthrosen, traumatisch induzierten sowie postoperativen Ergüssen

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