1990
DOI: 10.1210/jcem-71-1-179
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Evidence for the Presence of Human Chorionic Gonadotropin (hCG) and Freeβ-Subunit of hCG in the Human Pituitary

Abstract: Previous studies have indicated that the pituitary gland may produce free alpha-subunit and small quantities of hCG in addition to other glycoprotein hormones. Since synthesis of holo-hCG requires the presence of both subunits, we have investigated the occurrence in human pituitary of free beta-subunit of hCG, in addition to intact holo-hCG. We processed a pituitary extract by fractionated ammonium sulfate precipitation followed by sequential chromatography on Sephadex G-100 and Ultrogel AcA 44. The fractions … Show more

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Cited by 63 publications
(28 citation statements)
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“…Monoclonal antibodies employed, i.e., 11/6 (against holo-hCG and LH), 3/6 (hCG and /3-subunit), 12/17 (hCG and /-subunit), and 2/6 (specific for the /3-subunit of hCG), were raised and purified by Dr. K. Siddle, University of Cambridge, UK, as reported previously (21). Assay procedures and assay characteristics have been described in detail in previous reports from our laboratory (22,23). The assay, which employs the monoclonal antibodies, 3/6 as solid phase antibody and 1 1/6 as tracer, measures holo-hCG with negligible cross-reactivity with free beta-subunit of hCG (< 0.01%).…”
mentioning
confidence: 99%
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“…Monoclonal antibodies employed, i.e., 11/6 (against holo-hCG and LH), 3/6 (hCG and /3-subunit), 12/17 (hCG and /-subunit), and 2/6 (specific for the /3-subunit of hCG), were raised and purified by Dr. K. Siddle, University of Cambridge, UK, as reported previously (21). Assay procedures and assay characteristics have been described in detail in previous reports from our laboratory (22,23). The assay, which employs the monoclonal antibodies, 3/6 as solid phase antibody and 1 1/6 as tracer, measures holo-hCG with negligible cross-reactivity with free beta-subunit of hCG (< 0.01%).…”
mentioning
confidence: 99%
“…The assay, which employs the monoclonal antibodies, 3/6 as solid phase antibody and 1 1/6 as tracer, measures holo-hCG with negligible cross-reactivity with free beta-subunit of hCG (< 0.01%). The second assay, using 2/6 as solid phase antibody and 12/17 as tracer, is specific for the free beta-subunit of hCG its cross-reactivity with holohCG being only 0.4% (23). Radioiodination (9).…”
mentioning
confidence: 99%
“…Immunoreactive human chorionic gonadotropin (hCG) is released by various trophoblastic as well as nontrophoblastic tumors and has been found in malignant pleural and peritoneal effusions [15,16,[18][19][20], We have recently detected hCG immunoreactivity in ascitic fluid [21]. This was apparently due to the presence of the free (3-subunit ofhCG rather than of the intact holo-hCG mol ecule.…”
Section: Introductionmentioning
confidence: 99%
“…As a result, continuous GnRH stimulation of gonadotrope cells occurs, leading to increased LH and FSH production. Under this hyperstimulation, the pituitary may secrete an hCG-like molecule (7)(8)(9). Pituitary hCG is the cause of increased serum hCG results in nonpregnant women, most notably in the peri-and postmenopausal periods (4 -9 ).…”
mentioning
confidence: 99%
“…Pituitary hCG is the cause of increased serum hCG results in nonpregnant women, most notably in the peri-and postmenopausal periods (4 -9 ). Not surprisingly, pituitary hCG is suppressed by estrogen/progestagen contraceptive pills (4,6,7,9 ).…”
mentioning
confidence: 99%