Evidence for the presence of β3-adrenergic receptor mRNA in the human brain

Rodriguez, Marianne; Carillon, Christine; Coquerel, Antoine; Fur, G. Le; Ferrara, Pascual; Caput, Daniel; Shire, David

doi:10.1016/0169-328x(94)00274-i

Cited by 55 publications

(39 citation statements)

References 19 publications

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“…In recent years, expression of the protein was also found in the human heart (Rozec and Gauthier , (Rodriguez et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

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Distribution and quantification of β-3 adrenergic receptor in tissues of sheep

Liu

et al. 2011

Animal

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The b-3 adrenergic receptor (ADRB3) is a G-protein coupled receptor involved in regulating lipolysis, as part of homeostatic regulation. In this study, South African Mutton Merino and Shanxi Dam Line were used to study the distribution and quantification of ADRB3 in adipose (subcutaneous, omental, retroperitoneal, mesenteric and perirenal fat) and non-adipose (heart, liver, spleen, lung and kidney) tissues of sheep. The protein was determined by immunohistochemical technique and by mRNA abundance via real-time polymerase chain reaction. ADRB3 was detected in all studied tissues with abundance in adipose tissues higher than in non-adipose tissues ( P , 0.001). For adipose tissues, greater expression was found in deep deposits such as great omental and retroperitoneal fat than in subcutaneous fat ( P , 0.05). Significant differences ( P , 0.05) both for mRNA and for protein expression also existed between the two sheep flocks. These findings are consistent with the known function of ADRB3 in mediating lipolysis and homeostasis in adipose tissues.Keywords: b-3 adrenergic receptor, sheep, tissues, mRNA expression, immunohistochemistry ImplicationsIn this study, the b-3 adrenergic receptor (ADRB3) expression in various tissues of sheep was detected by immunohistochemical technique and by mRNA abundance via real-time PCR. Despite its low expression, the presence of ADRB3 in the spleen and kidney was found in livestock for the first time. Generally, ADRB3 expressed higher in adipose than in non-adipose tissues, and for the former, it was higher in deep fat deposits than in subcutaneous fat. The distributions are consistent with the known functions of ADRB3 in mediating lipolysis and homeostasis in adipose tissues, and relaxation of vascular smooth muscle in the cardiovascular system. The relation between low expression and its function in some viscera, for example, lung, spleen and kidney of sheep deserves further study.Introduction b-Adrenergic receptors (ADRBs) are G-protein coupled receptors that mediate effects of the endogenous catecholamines adrenaline and noradrenaline. Three subtypes of ADRBs have been identified, named ADRB1, ADRB2 and ADRB3, respectively. These receptors are present in most mammalian tissues, but the distribution of each subtype varies among tissues in a given species. Among the various physiological roles of ADRBs, the ADRB3 subtype mediates lipolysis and stimulates thermogenesis in adipose tissues (Cannon and Nedergaard, 2004) by activating uncoupling protein in mitochondria.In early studies, ADRB3 was identified mainly on the surface of white and brown adipocytes as reviewed by Strosberg (1997). In recent years, expression of the protein was also found in the human heart (Rozec and Gauthier , (Rodriguez et al., 1995).It has been confirmed that the mRNA of the ADRB3 gene is expressed in various tissues of domestic animals. The 2.0 kb ADRB3 transcript in the bovine brown adipose tissue (PietriRouxel et al., 1995), and two transcripts of 2.2 and 1.9 kb in porcine subcutaneous fat (McNeel and ...

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“…In recent years, expression of the protein was also found in the human heart (Rozec and Gauthier , (Rodriguez et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, expression of the protein was also found in the human heart (Rozec and Gauthier, 2006), mammary artery (Rozec et al, 2005), placental artery (Rouget et al, 2006), gastrointestinal tract (Anthony et al, 2000), prostate and gall bladder (de Matteis et al, 2002), urinary bladder detrusor (Igawa et al, 1999) and brain (Rodriguez et al, 1995).…”

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confidence: 98%

Distribution and quantification of β-3 adrenergic receptor in tissues of sheep

Liu

et al. 2011

Animal

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“…Initially, the existence of the b 3 adrenoceptor in the brain was questioned, as classical binding studies had failed to detect its presence in central tissues. However, more recently, experiments using reverse transcription/PCR revealed the existence of b 3 -adrenoceptor mRNA in discrete regions of the rat and human brain, including hippocampus, hypothalamus, amygdala, and cerebral cortex, areas known to be involved in the modulation of stress-related behaviors (Rodriguez et al, 1995;Summers et al, 1995;Strosberg, 1997). Furthermore, the availability of the selective b 3 -adrenoceptor agonist SR58611A (amibegron) has opened new avenues for exploring the possible involvement of the b 3 adrenoceptor in the modulation of stress-related disorders Manara and Bianchetti, 1990).…”

Section: Introductionmentioning

confidence: 99%

Stimulation of the β3-Adrenoceptor as a Novel Treatment Strategy for Anxiety and Depressive Disorders

Stemmelin

Cohen

Terranova

et al. 2007

Neuropsychopharmacol

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109

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The characterization of the first selective orally active and brain-penetrant b 3 -adrenoceptor agonist, SR58611A (amibegron), has opened new possibilities for exploring the involvement of this receptor in stress-related disorders. By using a battery of tests measuring a wide range of anxiety-related behaviors in rodents, including the mouse defense test battery, the elevated plus-maze, social interaction, stressinduced hyperthermia, four-plate, and punished drinking tests, we demonstrated for the first time that the stimulation of the b 3 receptor by SR58611A resulted in robust anxiolytic-like effects, with minimal active doses ranging from 0.3 to 10 mg/kg p.o., depending on the procedure. These effects paralleled those obtained with the prototypical benzodiazepine anxiolytic diazepam or chlordiazepoxide.Moreover, when SR58611A was tested in acute or chronic models of depression in rodents, such as the forced-swimming and the chronic mild stress tests, it produced antidepressant-like effects, which were comparable in terms of the magnitude of the effects to those of the antidepressant fluoxetine or imipramine. Supporting these behavioral data, SR58611A modified spontaneous sleep parameters in a manner comparable to that observed with fluoxetine. Importantly, SR58611A was devoid of side effects related to cognition (as shown in the Morris water maze and object recognition tasks), motor activity (in the rotarod), alcohol interaction, or physical dependence. Antagonism studies using pharmacological tools targeting a variety of neurotransmitters involved in anxiety and depression and the use of mice lacking the b 3 adrenoceptor suggested that these effects of SR58611A are mediated by b 3 adrenoceptors. Taken as a whole, these findings indicate that the pharmacological stimulation of b 3 adrenoceptors may represent an innovative approach for the treatment of anxiety and depressive disorders.

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“…Krief et al 17 detected b 3 -adrenoceptor mRNA in several tissues, including gall bladder, adipose tissue and colon, while Thomas and Liggett gall bladder, stomach, small intestine, adipose tissue and prostate gland. 19 In addition, b 3 -adrenoceptor mRNA has been reported in human brain, 20 although this was not detected by others. 19 These con¯icting results may, in part, be due to the relatively low levels of b 3 -adrenoceptor gene expression in human tissue.…”

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confidence: 99%

The tissue distribution of the human β3-adrenoceptor studied using a monoclonal antibody: Direct evidence of the β3-adrenoceptor in human adipose tissue, atrium and skeletal muscle

et al. 1999

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The tissue distribution of the human b b 3 -adrenoceptor studied using a monoclonal antibody: Direct evidence of the b b 3 -adrenoceptor in human adipose tissue, atrium and skeletal muscle 2,3 The human b 3 -adrenoceptor was later cloned by Emorine et al. 4 The pharmacology of the cloned b 3 -adrenoceptor agreed with the pharmacological data previously obtained in rodent adipose tissue, gut, and skeletal muscle in that the badrenoceptors in these tissues were insensitive to classical b-adrenoceptor antagonists such as propranolol.5 ± 8 Aryloxypropanolamine b 1 ab 2 adrenoceptor antagonists, exempli®ed by CGP12177, evoke a lipolytic response in rat adipose tissue through agonism at b 3 -adrenoceptors. 9 Similarly, selective b 3 -adrenoceptor agonists, such as BRL-37344, showed similar or greater potency than isoproterenol in stimulating lipolysis, but were much less potent than isoproterenol in stimulating responses mediated by b 1 -or b 2 -adrenoceptors. 10 The assessment of the pharmacological role of b 3 -adrenoceptors in human tissues has proved more controversial. For example, lipolysis in human white adipocytes induced by isoproterenol is sensitive to propranolol.11 Also, CGP12177-induced lipolytic responses have been demonstrated by some 12 ± 14 but not others. 9,15 It is now evident from comparisons of cloned human and rat b 3 -adrenoceptors that there are signi®cant species differences in pharmacology. 16 Attempts to detect b 3 -mRNA in human tissues using reverse-transcription PCR have also given conicting results. Krief et al 17 detected b 3 -adrenoceptor mRNA in several tissues, including gall bladder, adipose tissue and colon, while Thomas and Liggett 18 failed to detect a b 3 -adrenoceptor signal. Recently, RNAase protection assays, that do not rely on ampli®cation techniques, were used to identify b 3 -adrenoceptor mRNA in a variety of human tissues, including

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Evidence for the presence of β3-adrenergic receptor mRNA in the human brain

Cited by 55 publications

References 19 publications

Distribution and quantification of β-3 adrenergic receptor in tissues of sheep

Distribution and quantification of β-3 adrenergic receptor in tissues of sheep

Stimulation of the β3-Adrenoceptor as a Novel Treatment Strategy for Anxiety and Depressive Disorders

The tissue distribution of the human β3-adrenoceptor studied using a monoclonal antibody: Direct evidence of the β3-adrenoceptor in human adipose tissue, atrium and skeletal muscle

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