Evidence for the presence of histamine in Gaboon viper (Bitis gabonica) venom

Tilmisany, A.K.; Mustafa, Ali A.; Aziz, Abdul; Osman, O.H.

doi:10.1016/0041-0101(86)90142-x

Cited by 5 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous studies have reported on the histamine-releasing capabilities of various snake venoms [ 219 , 224 , 225 , 226 , 227 ], based upon their capacity to degranulate mast cells [ 228 , 229 , 230 ]; however, to the best of our knowledge, histamine has been reported as an actual component of a snake venom only once ( Bitis gabonica ) [ 231 ]. Recently, Mamede et al [ 232 ] reported that the inflammatory reaction caused by Bothrops moojeni venom is mediated by eicosanoids, histamine, nitric oxide, and bradykinin, principally due to the action of phospholipases, metalloproteases, and serine proteases.…”

Section: Resultsmentioning

confidence: 99%

Organic and Peptidyl Constituents of Snake Venoms: The Picture Is Vastly More Complex Than We Imagined

Villar‐Briones

Aird

2018

Toxins

View full text Add to dashboard Cite

Small metabolites and peptides in 17 snake venoms (Elapidae, Viperinae, and Crotalinae), were quantified using liquid chromatography-mass spectrometry. Each venom contains >900 metabolites and peptides. Many small organic compounds are present at levels that are probably significant in prey envenomation, given that their known pharmacologies are consistent with snake envenomation strategies. Metabolites included purine nucleosides and their bases, neurotransmitters, neuromodulators, guanidino compounds, carboxylic acids, amines, mono- and disaccharides, and amino acids. Peptides of 2–15 amino acids are also present in significant quantities, particularly in crotaline and viperine venoms. Some constituents are specific to individual taxa, while others are broadly distributed. Some of the latter appear to support high anabolic activity in the gland, rather than having toxic functions. Overall, the most abundant organic metabolite was citric acid, owing to its predominance in viperine and crotaline venoms, where it chelates divalent cations to prevent venom degradation by venom metalloproteases and damage to glandular tissue by phospholipases. However, in terms of their concentrations in individual venoms, adenosine, adenine, were most abundant, owing to their high titers in Dendroaspis polylepis venom, although hypoxanthine, guanosine, inosine, and guanine all numbered among the 50 most abundant organic constituents. A purine not previously reported in venoms, ethyl adenosine carboxylate, was discovered in D. polylepis venom, where it probably contributes to the profound hypotension caused by this venom. Acetylcholine was present in significant quantities only in this highly excitotoxic venom, while 4-guanidinobutyric acid and 5-guanidino-2-oxopentanoic acid were present in all venoms.

show abstract

Section: Resultsmentioning

confidence: 99%

Organic and Peptidyl Constituents of Snake Venoms: The Picture Is Vastly More Complex Than We Imagined

Villar‐Briones

Aird

2018

Toxins

View full text Add to dashboard Cite

show abstract

“…In contrast to the several studies on the role of histamine in the envenomation of scorpions, snakes and bees, only few studies focus on the participation of histamine in that caused by caterpillars ( 26 – 28 ). Shama et al (1982) and Dinehart et al (1987) used an enzymatic isotopic assay to quantify 80 ng histamine for the caterpillar of the gypsy moth, Lymantria dispar , and 2,7 µM histamine for that of the yellowtail moth, Hylesia spp ( 29 , 30 ).…”

Section: Discussionmentioning

confidence: 99%

Unraveling the venom chemistry with evidence for histamine as key regulator in the envenomation by caterpillar Automeris zaruma

et al. 2022

View full text Add to dashboard Cite

Over the past decades, envenomation by caterpillars of Automeris spp. became an increasing health problem in Latin America. Accidental contact with the stinging spines of these caterpillars cause acute local pain, itching, inflammation and skin rashes that persists for days. Even when the cause is obvious, the exact molecular mechanisms responsible for the observed symptoms are yet to be elucidated. Here, we describe for the first time, an active compound in the venom and the study of the bioactivity of the venom extracted from the spines of the caterpillar Automeris zaruma. Electrophysiological screening of a library of membrane proteins important for pain and itch enabled us to investigate and reveal the mode of action of the venom of A. zaruma. Further mass spectrometric analysis (Q-TOF-MS) made it possible to establish a link between the bioactivity and the components found in the venom. We show that the spine extract of A. zaruma contains histamine that potently activates the four types of the human histamine receptors (H1R, H2R, H3R and H4R) with a selectivity preference towards H3R and H4R. Furthermore, a modulation of the target MRGPRX2 was found. Together, these findings are the first to explain the symptomology of A. zaruma envenomation, enabling us a better understanding of caterpillar envenomation and predict that the hurdle of the scarce efficacy of the currently used antihistaminic drugs can be overcome by including H3R and H4R blockers in the clinical used medication. Such an approach might be used for other caterpillar envenomation in the world and represent a significant improvement for the well-being of the patient.

show abstract

Acidotic effect of Gaboon viper (Bitis gabonica) venom in the urethane-anaesthetized rat

Gattullo

Hyslop

Marsh

et al. 1991

General Pharmacology: The Vascular System

View full text Add to dashboard Cite

Evidence for the presence of histamine in Gaboon viper (Bitis gabonica) venom

Cited by 5 publications

References 6 publications

Organic and Peptidyl Constituents of Snake Venoms: The Picture Is Vastly More Complex Than We Imagined

Organic and Peptidyl Constituents of Snake Venoms: The Picture Is Vastly More Complex Than We Imagined

Unraveling the venom chemistry with evidence for histamine as key regulator in the envenomation by caterpillar Automeris zaruma

Acidotic effect of Gaboon viper (Bitis gabonica) venom in the urethane-anaesthetized rat

Contact Info

Product

Resources

About