Evidence for the presence of N‐CAM 180 on astrocytes from rat cerebellum and differences in glycan structures between N‐CAM 120 and N‐CAM 140

Sasaki, Takaya; Endo, Tamao

doi:10.1002/(sici)1098-1136(199912)28:3<236::aid-glia7>3.0.co;2-#

Cited by 14 publications

(8 citation statements)

References 49 publications

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“…Donato, 2001; Marenholz et al, 2004). It would, therefore, be interesting to compare the location of binding of anti‐GS and anti‐S100 antibodies in the crustacean visual system with that of Datura stramonium lectin (DSL), a tested marker of both arthropod glia and rat cerebellar astrocytes (Hählein et al, 1996; Jacobs and Lakes‐Harlan, 1997; Sasaki and Endo, 1999).…”

Section: Introductionmentioning

confidence: 99%

Regionally specific distribution of the binding of anti‐glutamine synthetase and anti‐S100 antibodies and of Datura stramonium lectin in glial domains of the optic lobe of the giant prawn

Allodi

Bressan

Carvalho

et al. 2006

Glia

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We previously characterized some crustacean glial cells by markers such as 2',3'-cyclic nucleotide 3'-phosphodiesterase and glial fibrillary acidic protein. Here we use antibodies against glutamine synthetase full-length molecule (anti-GS/FL), a GS C-terminal peptide (anti-GS/20aa-C), and brain S100 (anti-S100), as well as the binding of the insect glia and rat astrocytic marker Datura stramonium lectin (DSL), in the optic lobe of the prawn Macrobrachium rosenbergii. All markers label the lamina ganglionaris cartridge region (lighter: anti-GS/FL; heavier: DSL). In addition, anti-GS/FL labels superficial somata of external and internal medullas and internal chiasm cells. Both anti-GS/20aa-C and anti-S100 label heavily the glial sheaths of the lamina ganglionaris. In addition, anti-S100 binds to the perineurial glia of medullary parenchymal vessels. Western blot analyses show that both anti-GS/FL and anti-GS/20aa-C bind mostly to a band of 50-55 kDa, compatible with a long isoform of vertebrate GS, and accessorily to a possible dimer and, in the case of anti-GS/20aa-C, to an ill-defined band of intermediate mass. Binding of anti-S100 is selective for a single band of about 68 kDa but shows no protein in the weight range of the canonical S100 protein superfamily. DSL reveals two bands of about 75 and about 120 kDa, thus within the range of maximal recognition for rat astrocytes. Our results suggest that phenotype protein markers of the optic lobe glia share antigenic determinants with S100 and (a long form of) GS and that, similarly to vertebrate and insect glia, crustacean glia protein and N-glycan residue markers display regional heterogeneity.

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Section: Introductionmentioning

confidence: 99%

Regionally specific distribution of the binding of anti‐glutamine synthetase and anti‐S100 antibodies and of Datura stramonium lectin in glial domains of the optic lobe of the giant prawn

Allodi

Bressan

Carvalho

et al. 2006

Glia

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“…The addition of PSA and HNK-1 to CAMs in the brain is both temporally and spatially regulated (1,8). As a result, only a fraction of any CAM is modified with either of these carbohydrate structures, indicating the synthesis of these glycoproteins and the carbohydrate structures that modify them are regulated independently.…”

mentioning

confidence: 99%

Spatial and Temporal Regulation of Tenascin-R Glycosylation in the Cerebellum

Woodworth

Fiete

Baenziger

2002

Journal of Biological Chemistry

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The cellular adhesion molecule tenascin-R is a multifunctional extracellular matrix component expressed exclusively in the central nervous system. The expression of tenascin-R by oligodendrocytes and small interneurons in the hippocampus and cerebellum is highly regulated during development of these regions. This complex glycoprotein displays both adhesive and antiadhesive properties that contribute to the formation and maintenance of synapses. We have determined that tenascin-R associated with Purkinje cell bodies and their dendrites in the molecular layer of the cerebellum bears N-linked oligosaccharides terminating with ␤1,4-linked GalNAc-4-SO 4 , whereas tenascin-R in other regions of the cerebellum does not bear this modification. Expression of this unique sulfated carbohydrate structure is also temporally regulated, increasing throughout cerebellar development. The most dramatic increase in GalNAc-4-SO 4 occurs between postnatal days 14 and 21, corresponding to a period of Purkinje cell dendrite extension and synaptogenesis. The spatially and temporally regulated addition of this unique sulfated carbohydrate to tenascin-R may serve to modulate its adhesive/anti-adhesive or other biological properties in vivo.

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“…Since lectins recognise specific sequences and configurations of the oligosaccharides, they are useful tools for distinguishing glycoproteins produced by different cells. In fact, DSA reacted strongly with many rat astrocyte glycoproteins, but reacted with very few neuronal glycoproteins (Sasaki and Endo, 1999). Actually, DSA did not affect neuronal cell migration or axonal extension and did not induce any morphological change of neurons (Sasaki and Endo, 2000).…”

Section: Discussionmentioning

confidence: 89%

Inhibition of proliferation and induction of differentiation of glioma cells with Datura stramonium agglutinin

et al. 2002

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We found that a lectin, Datura stramonium agglutinin, induced irreversible differentiation in C6 glioma cells. The differentiated cells had long processes, a low rate of proliferation and a high content of glial fibrillary acidic protein. When the medium was replaced with Datura stramonium agglutinin-free medium after 1 h, cell proliferation continued to be inhibited. Experiments with several other lectins indicated that both recognition of linear N-acetyllactosamine repeats and recognition of multiantennary units of cell-surface glycans were required for the inhibition of C6 proliferation. Proliferation of four human glial tumour cells was also inhibited by Datura stramonium agglutinin. Further, these differentiated human glial tumour cells had long processes and a high content of glial fibrillary acidic protein similar to differentiated C6 glioma cells. Taken together, these observations suggest that Datura stramonium agglutinin may be useful as a new therapy for treating glioma without side effects.

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Evidence for the presence of N‐CAM 180 on astrocytes from rat cerebellum and differences in glycan structures between N‐CAM 120 and N‐CAM 140

Cited by 14 publications

References 49 publications

Regionally specific distribution of the binding of anti‐glutamine synthetase and anti‐S100 antibodies and of Datura stramonium lectin in glial domains of the optic lobe of the giant prawn

Regionally specific distribution of the binding of anti‐glutamine synthetase and anti‐S100 antibodies and of Datura stramonium lectin in glial domains of the optic lobe of the giant prawn

Spatial and Temporal Regulation of Tenascin-R Glycosylation in the Cerebellum

Inhibition of proliferation and induction of differentiation of glioma cells with Datura stramonium agglutinin

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