Evidence for the presence of a histaminergic neuron system in the rat brain: An immunohistochemical analysis

Watanabe, Takehiko; Taguchi, Yoshitaka; Hayashi, Hideyuki; Tanaka, Junya; Shiosaka, Sadao; Tohyama, Masaya; Kumakura, Hiroaki; Terano, Yoshitake; Wada, Hiroshi

doi:10.1016/0304-3940(83)90308-7

Cited by 203 publications

(89 citation statements)

References 13 publications

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“…Furthermore, they describe numerous immunoreactive nerve fibers in the median eminence, whereas in our study the immunoreactivity to histamine in the median eminence was found mainly in mast cells, and only a very few immunoreactive fibers were detected. Our results support a biochemical study by Pollars et al (4) (25) detected histidine decarboxylase-like immunoreactivity in the enterochromaffin-like cells of the stomach in agreement with our results with antiserum against histamine, but they report neuronal cell bodies in the arcuate nucleus, which in our study was devoid of histamine-immunoreactive cells. We found the cells more laterally in the caudal magnocellular nucleus and around the ventral premammillary nucleus.…”

Section: Methodssupporting

confidence: 83%

Histamine-containing neurons in the rat hypothalamus.

Panula¹,

Yang²,

Costa³

1984

Proc. Natl. Acad. Sci. U.S.A.

629

318

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A specific antiserum against histamine was produced in rabbits, and an immunohistochemical study of histamine-containing cells was carried out in rat brain. The antiserum bound histamine in a standard radioimmunoassay and stained mast cells located in various rat and guinea pig tissues. Enterochromaffin-like cells in the stomach and neurons in the posterior hypothalamic area could be detected with this antiserum. The staining was highly specific and was not abolished by preabsorption with histidine, histidine-containing peptides, serotonin, or catecholamines, whereas preabsorption with histamine completely abolished the staining. Immunoglobulins of this antiserum purified by affinity chromatography stained the same cells as did the crude antiserum, whereas the serum fraction, which was not absorbed by histamine-affinity ligand, failed to stain any neuron. Histamine-immunoreactive neuronal cell bodies were found only in the hypothalamic and premammillary areas of colchicine-treated rats. The largest group of cells was seen in the caudal magnocellular nucleus and medially on the dorsal and ventral aspects of the ventral premammillary nucleus. Immunoreactive nerve fibers, but no cell bodies, were detected in other parts of the brain. Histamine-immunoreactive mast cells were found in the median eminence and pituitary gland. The results suggest that histamine-containing neurons are located only in a small area of the posterior hypothalamus, and these cells are probably the source of ascending and descending fibers detected in other brain areas.During the last decade, a number of studies have indicated that histamine may function as a putative neurotransmitter in the mammalian central nervous system (1). In the brain, the highest histamine content is found in hypothalamus (2-5) and in certain areas of the mesencephalon (6). Biochemical measurements of histamine and its synthesizing enzyme, histidine decarboxylase, in various regions of intact and lesioned brains suggest that brain contains long axon histamine-containing neurons. Furthermore, although a considerable portion of brain histamine appears to be located in mast cells (4,7,8), its presence in synaptosomal fractions suggests its neuronal location (9-12); this is supported by the presence in brain structures of adenylate cyclase activity stimulated by histamine (13).The immunocytochemical method that detects serotonin has been useful in revealing serotonin-containing neuronal pathways in the central nervous system (14). Attempts to develop a fluorescence histochemical method for histamine have been made (15-17), but the o-phthalylaldehyde (OPT) method failed to detect histamine-containing neurons in the brain. Wilcox and Seybold (18) reported the presence of histamine-containing neurons in the median eminence, using antibodies against histamine. However, this study detected only a few histamine-containing cells. The present study, in which an antiserum against histamine is used, shows that several groups of histamine-containing neurons are located in ra...

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Section: Methodssupporting

confidence: 83%

Histamine-containing neurons in the rat hypothalamus.

Panula¹,

Yang²,

Costa³

1984

Proc. Natl. Acad. Sci. U.S.A.

629

318

View full text Add to dashboard Cite

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“…Interactions among histaminergic and other neurotransmitter systems regulate the sleep-wake cycle Several experimental observations support the hypothesis that the histaminergic system constitutes a major wakepromoting system, as its terminals influence neuronal excitability in several brain areas [1,2]. Direct electrophysiological recordings from freely moving cats showed that the activity of histaminergic neurons is high during waking and low or absent during sleep [3], and their firing rate changes with the behavioral state [4].…”

mentioning

confidence: 83%

Histamine in the brain: Beyond sleep and memory

Passani¹,

Giannoni²,

Bucherelli

et al. 2007

Biochemical Pharmacology

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“…The central histaminergic neurons, located in the tuberomammillary nucleus in the posterior hypothalamus (Watanabe et al, 1983;Panula et al, 1984), have widely distributed projections throughout the brain regulating a broad range of physiological functions such as sleep-wake cycle, energy and endocrine homeostasis, and memory processes (Haas and Panula, 2003).…”

Section: Introductionmentioning

confidence: 99%

Histaminergic Neurons Protect the Developing Hippocampus from Kainic Acid-Induced Neuronal Damage in an Organotypic Coculture System

Kukko‐Lukjanov

Soini²,

Taira³

et al. 2006

J. Neurosci.

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The central histaminergic neuron system inhibits epileptic seizures, which is suggested to occur mainly through histamine 1 (H 1 ) and histamine 3 (H 3 ) receptors. However, the importance of histaminergic neurons in seizure-induced cell damage is poorly known. In this study, we used an organotypic coculture system and confocal microscopy to examine whether histaminergic neurons, which were verified by immunohistochemistry, have any protective effect on kainic acid (

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Evidence for the presence of a histaminergic neuron system in the rat brain: An immunohistochemical analysis

Cited by 203 publications

References 13 publications

Histamine-containing neurons in the rat hypothalamus.

Histamine-containing neurons in the rat hypothalamus.

Histamine in the brain: Beyond sleep and memory

Histaminergic Neurons Protect the Developing Hippocampus from Kainic Acid-Induced Neuronal Damage in an Organotypic Coculture System

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