Evidence for the requirement of extracellular protease in the pathogenic interaction of Pyrenopeziza brassicae with oilseed rape

Ball, Alison M.; Ashby, Alison M.; Daniels, Michael J.; Ingram, D. S.; Johnstone, Keith

doi:10.1016/s0885-5765(05)80132-8

Cited by 65 publications

(29 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In certain cases, the activity of extracellular proteinases of a pathogenic microorganism is directly related to the intensity of the disease it induces in the plants. For example, pathogenic strains of the fungus Pyrenopeziza brassicae differ from its nonpathogenic counterparts by the ability to secrete a cysteine proteinase [332]. An aspartic proteinase secreted by the fungus Botrytis cinerea induces death in plant cells.…”

Section: Physiological Functionsmentioning

confidence: 99%

Proteinase inhibitors and their function in plants: A review

Mosolov

Valueva

2005

Appl Biochem Microbiol

112

View full text Add to dashboard Cite

show abstract

Section: Physiological Functionsmentioning

confidence: 99%

Proteinase inhibitors and their function in plants: A review

Mosolov

Valueva

2005

Appl Biochem Microbiol

112

View full text Add to dashboard Cite

show abstract

“…These include the enzymes produced by Botrytis cinerea [25], Cryphonectria parasitica (endothia pepsin) [26], and Glomerella cingulata [27]. Cysteine pro teinase is secreted by the fungus Pyrenopeziza brassicae [28]. Metalloproteinases include a family of Zn depend ent bacterial enzymes belonging to the genus Erwinia [29 31].…”

mentioning

confidence: 99%

Role of inhibitors of proteolytic enzymes in plant defense against phytopathogenic microorganisms

Valueva

Mosolov

2004

Biochemistry (Moscow)

View full text Add to dashboard Cite

This review analyzes the literature on various mechanisms of proteolytic enzyme inhibitors involved in plant defense against attack by phytopathogenic microorganisms. The action of proteinase inhibitors from plants upon the enzymes from pathogenic microorganisms and viruses is reviewed. Considerable attention is given to the induction of proteinase inhibitors in plants in response to the invasion of pathogens. Some aspects of application of proteinase inhibitors in biotechnology for production of transgenic plants with enhanced resistance to diseases are discussed.

show abstract

“…Recently, Ball etal. [4] reported the requirement of an extracellular cysteine proteinase in the pathogenic interaction of the fungus Pyrenopeziza brassicae with oil seed rape.…”

Section: Discussionmentioning

confidence: 99%

Expression of a cysteine proteinase inhibitor (oryzacystatin-I) in transgenic tobacco plants

et al. 1993

View full text Add to dashboard Cite

Expression of cysteine proteinase inhibitors (cystatins) in tobacco or other plants has the potential for improving resistance against pathogens and insects that possess cysteine proteinases. A chimeric gene containing a cDNA clone of rice cystatin (oryzacystatin-I; OC-I), the cauliflower mosaic virus 35S promoter, and the nopaline synthase 3' region was introduced into tobacco plants by Agrobacterium tumefaciens. The presence of the chimeric gene in transgenic plants was detected by a polymerase chain reaction-amplified assay, and transcriptional activity was shown by RNA blot analysis. Heated extracts from transgenic tobacco plants, as well as from progeny which were obtained by selfing a primary transformant, contained protein bands that corresponded in molecular mass to OC-I and reacted with antibodies raised against rOC, a recombinant OC-I protein produced by Escherichia coli. Similar bands were absent in extracts from untransformed control plants. OC-I levels reached 0.5% and 0.6% of the total soluble proteins in leaves and roots, respectively, of some progeny. On a fresh weight basis, the OC-I content was higher in leaves (50 micrograms/g) than in roots (30 micrograms/g). OC-I was partially purified from protein extracts of rice seeds and from transgenic tobacco leaves by affinity to anti-rOC antibodies. OC-I from both sources was active against papain.

show abstract

Evidence for the requirement of extracellular protease in the pathogenic interaction of Pyrenopeziza brassicae with oilseed rape

Cited by 65 publications

References 28 publications

Proteinase inhibitors and their function in plants: A review

Proteinase inhibitors and their function in plants: A review

Role of inhibitors of proteolytic enzymes in plant defense against phytopathogenic microorganisms

Expression of a cysteine proteinase inhibitor (oryzacystatin-I) in transgenic tobacco plants

Contact Info

Product

Resources

About