Evidence for Training-Induced Changes in miRNA Levels in the Skeletal Muscle of Patients With Type 2 Diabetes Mellitus

Simaitis, Sarah; Schulte-Körne, Benedikt; Schiffer, Thorsten; Bloch, Wilhelm; Predel, Hans‐Georg; Brixius, Klara; Brinkmann, Christian

doi:10.3389/fphys.2020.599651

Cited by 9 publications

(8 citation statements)

References 30 publications

(47 reference statements)

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“…Many ndings con rmed the expression of microRNAs in skeletal muscle of T2DM patients and animal models (39,51,52), but with little information on miRNAs as a therapeutic target in diabetes. In this study, we investigaed the expression pro les of miR-133a in skeletal muscle of non-diabetic and diabetic rats following aerobic exercise (HIIT and MCT) alone and in combination with metformin.…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Metformin and exercise reduce miR-133a expression in skeletal muscles of diabetic rats

Alivaisi

Amini

Haghani

et al. 2023

Preprint

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Background Exercise training regulates the expression of miRs in diabetes. We investigated the effects of metformin alone or in combination with high-intensity interval training (HIIT) and moderate continuous training (MCT) on miR-133a expression in skeletal muscles of diabetic rats. Sprague-Dawley rats were randomly allocated into nine groups (n = 10 per group): control group (CG), control group + MCT group (CMTG), control group + HIIT group (CHTG), diabetic control group (CDG), diabetic + metformin group (DMG), diabetic + HIIT group (DHTG), diabetic + MCT group (DMTG), diabetic + metformin and MCT group (DMMTG), diabetic + metformin and HIIT group (DMHTG). Type 2 diabetes mellitus was induced in Sprague-Dawley rats and exercise protocols were performed for eight weeks. MiR-133a was extracted from skeletal muscles and their expression profile was determined by real-time reverse-transcription polymerase chain reaction (RT-PCR). Results The expression of miR-133a was increased in skeletal muscles of sedentary diabetic rats (CDG) in comparison to treated (CMTG,CHTG,DMG, DMTG) and healthy controls (CG). Treatment with either HIIT and MCT reduced miR-133a expression, but the combination of HIIT and MCT caused to a further reduction of miR-133a expression in diabetic rats. Conclusion The increased expression of MiR-133a in skeletal muscles of rats can be attenuated by metformin and / or HIIT and MCT. This presents an intervention target that could be used in preventing, treating, and/or delaying diabetes.

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Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Metformin and exercise reduce miR-133a expression in skeletal muscles of diabetic rats

Alivaisi

Amini

Haghani

et al. 2023

Preprint

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“…Furthermore, Simaitis evidenced that miRNA-29b-3p, miRNA-29c-3p, and miRNA-135a-5p expression were downregulated in the skeletal muscle of T2DM patients after 12 weeks of AT (70-80% of peak heart rate) [32].…”

Section: Type 2 Diabetes Mellitus (T2dm)mentioning

confidence: 99%

“…Instead, RT upregulated miR-23a and miR-195 suggests an involvement of these miRNAs in blood vessel development [31]. Another study investigating skeletal muscles' miRNA samples illustrated that miRNA-29b-3p, miRNA-29c-3p, and miRNA-135a-5p levels were downregulated after 12 weeks of aerobic training [32]. Moreover, miR-29b was downregulated following combined RT and HIIT performed on separate days [29].…”

Section: Type 2 Diabetes Mellitus (T2dm)mentioning

confidence: 99%

“…Moreover, miR-29b was downregulated following combined RT and HIIT performed on separate days [29]. Some of these miRNAs (29b-3p, 29c-3p, and 135a-5p) were found to negatively affect glucose metabolism [32]. Therefore, physical activity may represent an important tool in order to regulate glycemia due to the downregulation of the mentioned miRNAs.…”

Section: Type 2 Diabetes Mellitus (T2dm)mentioning

confidence: 99%

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The Effect of Physical Activity/Exercise on miRNA Expression and Function in Non-Communicable Diseases—A Systematic Review

Afzal,

Greco,

Quinzi

et al. 2024

IJMS

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Exercise may differently affect the expression of key molecular markers, including skeletal muscle and circulating miRNAs, involved in cellular and metabolic pathways’ regulation in healthy individuals and in patients suffering from non-communicable diseases (NCDs). Epigenetic factors are emerging as potential therapeutic biomarkers in the prognosis and treatment of NCDs and important epigenetic factors, miRNAs, play a crucial role in cellular pathways. This systematic review aims to underline the potential link between changes in miRNA expression after different types of physical activity/exercise in some populations affected by NCDs. In June 2023, we systematically investigated the following databases: PubMed, MEDLINE, Scopus, and Web of Science, on the basis of our previously established research questions and following the PRISMA guidelines. The risk of bias and quality assessment were, respectively, covered by ROB2 and the Newcastle Ottawa scale. Of the 1047 records extracted from the initial search, only 29 studies were found to be eligible. In these studies, the authors discuss the association between exercise-modulated miRNAs and NCDs. The NCDs included in the review are cancer, cardiovascular diseases (CVDs), chronic obstructive pulmonary disease (COPD), and type 2 diabetes mellitus (T2DM). We evidenced that miR-146, miR-181, miR-133, miR-21, and miRNA-1 are the most reported miRNAs that are modulated by exercise. Their expression is associated with an improvement in health markers and they may be a potential target in terms of the development of future therapeutic tools.

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“…Aerobic exercise (cycling or exercising on an elliptical crosstrainer) induced a decrease in the expression of miR-29b-3p, miR-29c-3p and miR-135a-5p post-training in the skeletal muscle of diabetic individuals, including the reduction of miR-29c-3p was correlated with improvement in cardiorespiratory fitness [112]. Swimming training, another type of aerobic exercise, also induced a change in the expression of another miRNA, miR-382 was overexpressed in the serum of diabetic mice after 12 weeks of training.…”

Section: Micrornas In T2dm and Physical Exercisementioning

confidence: 99%

MicroRNAs in type 2 diabetes mellitus: potential role of physical exercise

Improta-Caria¹,

Sousa²,

Roever³

et al. 2022

Rev. Cardiovasc. Med.

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Type 2 diabetes mellitus (T2DM) is a multifactorial metabolic disease, and its prevalence has grown worldwide. Several pathophysiological processes contribute to the development, progression and aggravating of the disease, for example, decreased insulin synthesis and secretion, insulin resistance, inflammation, and apoptosis, all these processes are regulated by various epigenetic factors, including microRNAs (miRNAs). MiRNAs are small non-coding RNAs, which are around 20 nucleotides in length and are regulators of gene expression at the post-transcriptional level, have a specific function of inhibiting or degrading a messenger RNA target. Thus, miRNAs modulate the expression of many associated genes with the pathophysiological processes in T2DM. On the other hand, miRNAs are also modulated through physical exercise (PE), which induces a change in their expression pattern during and after exercise. Some scientific evidence shows that PE modulates miRNAs beneficially and improves the signaling pathway of insulin resistance, however, little is known about the function of PE modulating miRNAs associated with the processes of insulin secretion, inflammation, and apoptosis. Thus, the objective of this review is to identify the miRNAs expression pattern in T2DM and compare it with the exercise-induced miRNAs expression pattern, identifying the signaling pathways that these miRNAs are regulating in the processes of insulin secretion, insulin resistance, inflammation, and apoptosis in T2DM, and how PE may have a potential role in modulating these signal transduction pathways, promoting benefits for patients with T2DM.

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Evidence for Training-Induced Changes in miRNA Levels in the Skeletal Muscle of Patients With Type 2 Diabetes Mellitus

Cited by 9 publications

References 30 publications

WITHDRAWN: Metformin and exercise reduce miR-133a expression in skeletal muscles of diabetic rats

WITHDRAWN: Metformin and exercise reduce miR-133a expression in skeletal muscles of diabetic rats

The Effect of Physical Activity/Exercise on miRNA Expression and Function in Non-Communicable Diseases—A Systematic Review

MicroRNAs in type 2 diabetes mellitus: potential role of physical exercise

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