Evidence for up-regulated low density lipoprotein receptor in human lung adenocarcinoma cell line A549

Emerging Roles of Apolipoprotein E and Apolipoprotein A-I in the Pathogenesis and Treatment of Lung Disease

“…Collectively, these studies suggest a role for apoE in promoting lung cancer, perhaps by enhancing cholesterol transport into tumor cells (50). Consistent with this concept, LDLR expression is up-regulated in the A549 human lung adenocarcinoma cell line and increases when cholesterol is depleted (53). Thus, in contrast to its protective effect regarding inflammatory respiratory diseases, apoE may promote tumor cell growth in the lung.…”

Coordinate up-regulation of low-density lipoprotein receptor and cyclo-oxygenase-2 gene expression in human colorectal cells and in colorectal adenocarcinoma biopsies

“…Caruso et al (1993) demonstrated the presence of ldlr in colorectal carcinoma by immuno-enzymatic assay in about 25% of patients studied. Gueddari et al (1993) showed up-regulation of ldlr mRNA in the human lung adenocarcinoma FIGURE 1 -LDLr-mediated uptake of DiI-labeled LDL in DiFi cells. LDL uptake is seen in the bright areas.…”

“…Certain tumors, such as colorectal carcinoma and human adenocarcinoma cell lines, have high levels of LDLr protein (Caruso et al, 1993;Gueddari et al, 1993). We have examined mRNA expression of ldlr and cox-2 in cell lines as well as in normal and tumor biopsies from patients with colorectal adenomas and adenocarcinomas.…”

Evaluation of Tributyrin Lipid Emulsion with Affinity to Low-Density Lipoprotein: Pharmacokinetics in Adult Male Wistar Rats and Cellular Activity on Caco-2 and HepG2 Cell Lines

“…After binding to the receptors, LDL is internalized via receptormediated endocytosis and degraded in lysosomes (Brown and Goldstein, 1986), and the cholesterol is liberated for use in the synthesis of steroid hormones and new plasma membranes. In the rapidly proliferating malignant cells, the increased need of cholesterol for new membrane synthesis may result in overexpression of the LDL receptor (Ho et al, 1978;Gueddari et al, 1993;Leppälä et al, 1995;Maletinska et al, 2000;Sviridov et al, 2003), which allows greater uptake of the lipoprotein by the neoplastic cells. Several investigators showed the uptake of LDL-drug complexes by malignant cells, leading to the possibility of using LDL as drug carrier in cancer chemotherapy (Maranhã o et al, 1994;Shawer et al, 2002;Chung and Wasan, 2004).…”