Evidence for wheat-rye nucleolar competition (amphiplasty) in triticale by silver-staining procedure

Lacadena, J. R.; Cermeño, M. C.; Orellana, Juan; Santos, Juan Luis

doi:10.1007/bf00317037

Cited by 125 publications

(55 citation statements)

References 50 publications

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“…C-banding and silver staining treatments were carried out according to Schwarzacher et al (1980) and Lacadena et a!. (1984), respectively, with minor modifications.…”

Section: Introductionmentioning

confidence: 99%

Chromosomal differentiation in Helianthus annuus var. macrocarpus: heterochromatin characterization and rDNA location

et al. 1996

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The 2n = 34 chromosomes of the inbred line HA89, and the Flamme and Mirasol hybrids of Helianthus annuus var. macrocarpus possess centromeric heterochromatin as established by Giemsa C-banding. This heterochromatin can not be differentiated by fluorochromes such as DAPI or Chromomycin A3, with selective affinity for specific DNA base pairs. This situation probably results from either a balanced AT/GC composition of the involved repeat or the existence of alternating repetitive sequences of opposite base pair composition in these heterochromatic areas. However, there is also heterochromatin associated with the secondary constrictions on three pairs of chromosomes. This heterochromatin appears to be GC-rich according to its response to the fluorochrome treatments, thus indicating heterochromatin heterogeneity in H. annuus. Silver staining reveals the existence of active NORs associated with these secondary constrictions. In situ hybridization with an rDNA probe confirms these results and makes the existence of other inactive rDNA sites unlikely. These results are relevant to evolutionary and breeding studies on sunflowers.

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“…C-banding and silver staining treatments were carried out according to Schwarzacher et al (1980) and Lacadena et a!. (1984), respectively, with minor modifications.…”

Section: Introductionmentioning

confidence: 99%

Chromosomal differentiation in Helianthus annuus var. macrocarpus: heterochromatin characterization and rDNA location

et al. 1996

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“…So in fact, there is a slight discrepancy between the observed rDNA sites and the estimate numbers of genomes. Changes of ribosomal sites were previously documented in interspecific hybrids and referred to as amphiplasty, i.e., morphological changes in chromosomes following interspecific hybridization (Lacadena et al 1984). These changes in the nucleolar activity as demonstrated by silver nitrate staining in wheat-rye hybrids visualize dynamics of rDNA transcription patterns.…”

Section: Discussionmentioning

confidence: 95%

Meiotic analysis and FISH with rDNA and rice BAC probes of the Thai KPS 01-01-25 sugarcane cultivar

et al. 2015

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The interspecific sugarcane hybrid ''KPS 01-01-25'' is one of Thailand's most successful cultivars, but its genetics and genomic constitution are greatly complicated due to the highly polyploid nature of this crop. Here we analyzed the crop's karyotype, studied chromosome pairing at meiosis I and performed fluorescent in situ hybridization (FISH) of rDNA and rice BACs as probes. Mitotic cell complements in early tapetal cells showed 110 well-spread chromosomes. Pollen mother cells at pachytene clearly demonstrated fully paired configurations of the long chromosomes, in which few pairing partner exchanges and loops could be discerned. In diakinesis cells we observed 74.1 % bivalents, 18.6 % univalents, 2.9 % trivalents and 4.4 % quadrivalents. During this stage secondary associations were abundant, but these threadlike structures disappear after metaphase I. FISH with 5S rDNA produced up to ten fluorescent spots, of which 2-5 are often very small, whereas the 45S rDNA probe showed foci on 8-9 chromosomes. Based on our results and that of related studies we estimate that this sugarcane is composed of 11-12 genomes. We also selected rice BACs containing chromosome 5 genes controlling economically important genes. Eight BACs with relatively strong dot-blot hybridization on genomic sugarcane DNA were selected for FISH on sugarcane chromosome slides. These crossspecies chromosome BAC FISH revealed weak signals, and most of the foci were distributed over parts of the chromosomes. Based on these experiments we concluded that cross-species FISH in sugarcane can better be carried out with sorghum BACs as probes.Keywords Cross-species chromosome painting Á Meiosis Á rDNA FISH Á Saccharum Á Sugarcane

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“…The C-banding procedure was carried out according to Giraldez et aL, (1979) and the silver-staining method for nucleolar organiser regions as described by Lacadena et aL, (1984). ja.…”

Section: Methodsmentioning

confidence: 99%

Nucleolar organiser activity in wheat-barley chromosome addition lines

1984

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SUMMARYThe nucleolar organiser activity of cultivated barley, Hordeum vulgare, and the wheat-barley chromosome addition lines is analysed by a modified, highly reproducible, silver-staining procedure. Four Ag-NORs are observed in metaphase cells of barley corresponding to chromosomes 5HV and 6Hv, Six Ag-NORs are detected in the chromosome addition lines 5HV and 6H" while only four AgNORs are observed in the rest of the addition lines analysed (2Hv, 3H, 4W and 7Hv). It is concluded that the nucleolar organiser chromosomes 1B, 6B, 5W and 6HV are active. The chromosome 6HV of barley seems to control the nucleolar activity of its homoeologous 6B of wheat judging from the diminution of the Ag-NOR size of chromosome 6B in the corresponding addition line.

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Evidence for wheat-rye nucleolar competition (amphiplasty) in triticale by silver-staining procedure

Cited by 125 publications

References 50 publications

Chromosomal differentiation in Helianthus annuus var. macrocarpus: heterochromatin characterization and rDNA location

Chromosomal differentiation in Helianthus annuus var. macrocarpus: heterochromatin characterization and rDNA location

Meiotic analysis and FISH with rDNA and rice BAC probes of the Thai KPS 01-01-25 sugarcane cultivar

Nucleolar organiser activity in wheat-barley chromosome addition lines

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