Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals

Xu, Fei; Marlétaz, Ferdinand; Gavriouchkina, Daria; Liu, Xiao; Sauka‐Spengler, Tatjana; Zhang, Guofan; Holland, Peter W.H.

doi:10.1038/s41467-021-23216-7

Cited by 12 publications

(9 citation statements)

References 74 publications

(80 reference statements)

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“…We find a particularly strong match between the exocrine pancreatic-like cells of the pluteus larvae and a cluster of gut related cells in the flatworm that, similar to the pluteus, are located at the top of the gut ( 8 ). This finding further confirms the presence of pancreatic-like cells in protostomes and is only the second occurrence in ciliated larvae ( 50 ).…”

Section: Discussionsupporting

confidence: 84%

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Single-cell atlases of two lophotrochozoan larvae highlight their complex evolutionary histories

Piovani

Leite

Yáñez-Guerra

et al. 2023

Preprint

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Pelagic larval stages are widespread across animals, yet it is unclear if larvae were present in the last common ancestor of animals or whether they evolved multiple times due to common selective pressures. Many marine larvae are at least superficially similar, they are small, swim through beating of ciliated bands and sense the environment with an apical organ structure. To understand these similarities, we have generated single cell atlases for marine larvae from two animal phyla and have compared their cell types. We found clear similarities among ciliary band cells and neurons of the apical organ in the two larvae pointing to possible homology of these structures suggesting a single origin of larvae within the clade analysed here (Lophotrochozoa). We also find several clade specific innovations in each larva, including distinct myocytes and shell gland cells in the oyster larva. Oyster shell gland cells express many novel genes which have made previous gene age estimates for trochophore larvae too young.

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Section: Discussionsupporting

confidence: 84%

“…4). Exocrine pancreatic-like cells have been described in adults of cephalochordates, tunicates, cnidarians and in the pacific oyster ( 50, 51 ). Previously they have only been described in larval stages of the sea urchin S. purpuratus ( 51 ).…”

Section: Resultsmentioning

confidence: 99%

Single-cell atlases of two lophotrochozoan larvae highlight their complex evolutionary histories

Piovani

Leite

Yáñez-Guerra

et al. 2023

Preprint

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“…The hepatopancreas is an organ with combined functions of the liver and pancreas ( Xu et al, 2021 ). The vertebrate hepatopancreas is also called the mid-intestinal gland ( Rőszer, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Full-Length Transcriptome Sequencing Reveals Tissue-Specific Gene Expression Profile of Mangrove Clam Geloina erosa

et al. 2022

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Mollusca is the second largest animal phylum and represents one of the most evolutionarily successful animal groups. Geloina erosa, a species of Corbiculidae, plays an important role in mangrove ecology. It is highly adaptable and can withstand environmental pollution and microbial infections. However, there is no reference genome or full-length transcriptome available for G. erosa. This impedes the study of the biological functions of its different tissues because transcriptome research requires reference genome or full-length transcriptome as a reference to improve accuracy. In this study, we applied a combination of Illumina and PacBio single-molecule real-time sequencing technologies to sequence the full-length transcriptomes of G. erosa tissues. Transcriptomes of nine samples obtained from three tissues (hepatopancreas, gill, and muscle) were sequenced using Illumina. Furthermore, we obtained 87,310 full-length reads non-chimeric sequences. After removing redundancy, 22,749 transcripts were obtained. The average Q score of 30 was 94.48%. In total, 271 alternative splicing events were predicted. There were 14,496 complete regions and 3,870 lncRNAs. Differential expression analysis revealed tissue-specific physiological functions. The gills mainly express functions related to filtration, metabolism, identifying pathogens and activating immunity, and neural activity. The hepatopancreas is the main tissue related to metabolism, it also involved in the immune response. The muscle mainly express functions related to muscle movement and control, it contains more energy metabolites that gill and hepatopancreas. Our research provides an important reference for studying the gene expression of G. erosa under various environmental stresses. Moreover, we present a reliable sequence that will provide an excellent foundation for further research on G. erosa.

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“…Here, we observed that site-specific genetic variants in the homeobox and HSP genes are essential regulators of body development and stress responses. As genetic divergence and its associated functional changes could strongly diversify the adaptive regulations and responses of oysters to environmental factors [28,[76][77][78], it is possible that the variance of environmental condition or source of oyster recruits between Shenzhen Bay and Lantau Island might lead to the possession of these genetic variants which warrants further investigation.…”

Section: Discussionmentioning

confidence: 99%

Population Genomics, Transcriptional Response to Heat Shock, and Gut Microbiota of the Hong Kong Oyster Magallana hongkongensis

Xie

Huang

Nong

et al. 2022

JMSE

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The Hong Kong oyster Magallana hongkongensis, previously known as Crassostrea hongkongensis, is a true oyster species native to the estuarine-coast of the Pearl River Delta in southern China. The species—with scientific, ecological, cultural, and nutritional importance—has been farmed for hundreds of years. However, there is only limited information on its genetics, stress adaptation mechanisms, and gut microbiota, restricting the sustainable production and use of oyster resources. Here, we present population structure analysis on M. hongkongensis oysters collected from Deep Bay and Lantau Island in Hong Kong, as well as transcriptome analysis on heat shock responses and the gut microbiota profile of M. hongkongensis oysters collected from Deep Bay. Single nucleotide polymorphisms (SNPs), including those on the homeobox genes and heat shock protein genes, were revealed by the whole genome resequencing. Transcriptomes of oysters incubated at 25 °C and 32 °C for 24 h were sequenced which revealed the heat-induced regulation of heat shock protein pathway genes. Furthermore, the gut microbe community was detected by 16S rRNA sequencing which identified Cyanobacteria, Proteobacteria and Spirochaetes as the most abundant phyla. This study reveals the molecular basis for the adaptation of the oyster M. hongkongensis to environmental conditions.

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Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals

Cited by 12 publications

References 74 publications

Single-cell atlases of two lophotrochozoan larvae highlight their complex evolutionary histories

Single-cell atlases of two lophotrochozoan larvae highlight their complex evolutionary histories

Full-Length Transcriptome Sequencing Reveals Tissue-Specific Gene Expression Profile of Mangrove Clam Geloina erosa

Population Genomics, Transcriptional Response to Heat Shock, and Gut Microbiota of the Hong Kong Oyster Magallana hongkongensis

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