Evidence of a bacterial core in the stored products pest <i>Plodia interpunctella</i>: the influence of different diets

Montagna, Matteo; Mereghetti, Valeria; Gargari, Giorgio; Guglielmetti, Simone; Faoro, Franco; Lozzia, G.C.; Locatelli, D.P.; Limonta, L.

doi:10.1111/1462-2920.13450

Cited by 44 publications

(69 citation statements)

References 62 publications

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“…In terms of the distribution of ASVs among phyla and families of bacteria, the alimentary canal bacteria of T. ni larvae feeding on collards appeared similar to other lepidopteran species (Jones et al, ; Paniagua Voirol, Frago, Kaltenpoth, Hilker, & Fatouros, ). The two most abundant ASVs from the midgut and other organs, apparently belong to the genera Cloacibacterium and Diaphorobacter , members of which have been previously reported from insect gut microbiomes (Montagna et al, ; Ravenscraft, Kish, Peay, & Boggs, ). Interestingly, a Diaphorobacter isolate closely related to the ASV from our study was able to utilize the organophosphate insecticide Triazophos as its sole carbon source (Yang et al, ), suggesting the possibility that it could contribute to insecticide resistance.…”

Section: Resultsmentioning

confidence: 79%

Cabbage looper (Trichoplusia ni Hübner) labial glands contain unique bacterial flora in contrast with their alimentary canal, mandibular glands, and Malpighian tubules

Lawrence¹,

Novak²,

Shao

et al. 2020

MicrobiologyOpen

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In recent years, several studies have examined the gut microbiome of lepidopteran larvae and how factors such as host plant affect it, and in turn, how gut bacteria affect host plant responses to herbivory. In addition, other studies have detailed how secretions of the labial (salivary) glands can alter host plant defense responses. We examined the gut microbiome of the cabbage looper (Trichoplusia ni) feeding on collards (Brassica oleracea) and separately analyzed the microbiomes of various organs that open directly into the alimentary canal, including the labial glands, mandibular glands, and the Malpighian tubules. In this study, the gut microbiome of T. ni was found to be generally consistent with those of other lepidopteran larvae in prior studies. The greatest diversity of bacteria appeared in the Firmicutes, Actinobacteria, Proteobacteria, and Bacteriodetes. Well‐represented genera included Staphylococcus, Streptococcus, Corynebacterium, Pseudomonas, Diaphorobacter, Methylobacterium, Flavobacterium, and Cloacibacterium. Across all organs, two amplicon sequence variants (ASVs) associated with the genera Diaphorobacter and Cloacibacterium appeared to be most abundant. In terms of the most prevalent ASVs, the alimentary canal, Malpighian tubules, and mandibular glands appeared to have similar complements of bacteria, with relatively few significant differences evident. However, aside from the Diaphorobacter and Cloacibacterium ASVs common to all the organs, the labial glands appeared to possess a distinctive complement of bacteria which was absent or poorly represented in the other organs. Among these were representatives of the Pseudomonas, Flavobacterium, Caulobacterium, Anaerococcus, and Methylobacterium. These results suggest that the labial glands present bacteria with different selective pressures than those occurring in the mandibular gland, Malpighian tubules and the alimentary canal. Given the documented effects that labial gland secretions and the gut microbiome can exert on host plant defenses, the effects exerted by the bacteria inhabiting the labial glands themselves deserve further study.

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Section: Resultsmentioning

confidence: 79%

Cabbage looper (Trichoplusia ni Hübner) labial glands contain unique bacterial flora in contrast with their alimentary canal, mandibular glands, and Malpighian tubules

Lawrence¹,

Novak²,

Shao

et al. 2020

MicrobiologyOpen

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“…A further possible explanation may rely on the different sequencing technologies adopted here in respect to Montagna et al . (), namely Illumina sequencing versus 454 pyrosequencing.…”

Section: Discussionmentioning

confidence: 99%

“…A further symbiont‐derived benefit regards the production of antimicrobial peptides, which protect the insect host against the invasion of foreign bacteria (e.g., Shao et al ., ). Almost all the previously reported studies focused on a single developmental stage (e.g., third instar larvae, Hernàndez‐Flores et al ., ; Snyman et al ., ; the adult stage, Montagna et al ., ), ignoring the changes that may occur of the microbiota structure along the life cycle of these holometabolous insects. To our knowledge, only two studies have investigated the microbiota's structure across the life cycle in lepidopterans.…”

Section: Introductionmentioning

confidence: 99%

Evidence for a conserved microbiota across the different developmental stages of Plodia interpunctella

et al. 2017

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Diversity and composition of lepidopteran microbiotas are poorly investigated, especially across the different developmental stages. To improve this knowledge, we characterize the microbiota among different developmental stages of the Indian meal moth, Plodia interpunctella, which is considered one of the major pest of commodities worldwide. Using culture-independent approach based on Illumina 16S rRNA gene sequencing we characterized the microbiota of four developmental stages: eggs, first-, and last-instar larvae, and adult. A total of 1022 bacterial OTUs were obtained, showing a quite diversified microbiota associated to all the analyzed stages. The microbiotas associated with P. interpunctella resulted almost constant throughout the developmental stages, with approximately 77% of bacterial OTUs belonging to the phylum of Proteobacteria. The dominant bacterial genus is represented by Burkholderia (∼64%), followed by Propionibacterium, Delftia, Pseudomonas, and Stenotrophomonas. A core bacterial community, composed of 139 OTUs, was detected in all the developmental stages, among which 112 OTUs were assigned to the genus Burkholderia. A phylogenetic reconstruction, based on the 16S rRNA, revealed that our Burkholderia OTUs clustered with Burkholderia cepacia complex, in the same group of those isolated from the hemipterans Gossyparia spuria and Acanthococcus aceris. The functional profiling, predicted on the base of the bacterial 16S rRNA, indicates differences in the metabolic pathways related to metabolism of amino acids between preimaginal and adult stages. We can hypothesize that bacteria may support the insect host during preimaginal stages.

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“…Conversely, enterococci and staphylococci have been proven to be dominant groups among the Firmicutes hosted in Lepidoptera guts (Montagna et al . ; Vilanova et al . ).…”

Section: Discussionmentioning

confidence: 99%

“…Actually, Montagna et al . () pointed out the relevant role of the host's diet in moulding the microbiota composition of the cosmopolitan moth.…”

Section: Discussionmentioning

confidence: 99%

Insect frass in stored cereal products as a potential source ofLactobacillus sanfranciscensisfor sourdough ecosystem

et al. 2017

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Evidence of a bacterial core in the stored products pest Plodia interpunctella: the influence of different diets

Cited by 44 publications

References 62 publications

Cabbage looper (Trichoplusia ni Hübner) labial glands contain unique bacterial flora in contrast with their alimentary canal, mandibular glands, and Malpighian tubules

Cabbage looper (Trichoplusia ni Hübner) labial glands contain unique bacterial flora in contrast with their alimentary canal, mandibular glands, and Malpighian tubules

Evidence for a conserved microbiota across the different developmental stages of Plodia interpunctella

Insect frass in stored cereal products as a potential source ofLactobacillus sanfranciscensisfor sourdough ecosystem

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