Evidence of a heterogeneous tissue oxygenation: renal ischemia/reperfusion injury in a large animal model

Crane, Nicole J.; Huffman, Scott W.; Alemozaffar, Mehrdad; Gage, Fred; Levin, Ira W.; Elster, Eric A.

doi:10.1117/1.jbo.18.3.035001

Cited by 23 publications

(16 citation statements)

References 24 publications

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“…For this technique, hyperspectral images (HSIs) in combination with darkfield imaging (DK) are utilized to characterize different subcellular components and approximate the locations of NPs. Over the past few years, hyperspectral imaging techniques have received considerable attention for single-cell analysis [19][20][21][22][23] . Both spectral and spatial information on NPs can be simultaneously recorded with hyperspectral imaging, providing a novel and label-free platform for the intracellular characterization of NPs.…”

Section: Introductionmentioning

confidence: 99%

Using intracellular plasmonics to characterize nanomorphology in human cells

2020

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Determining the characteristics and localization of nanoparticles inside cells is crucial for nanomedicine design for cancer therapy. Hyperspectral imaging is a fast, straightforward, reliable, and accurate method to study the interactions of nanoparticles and intracellular components. With a hyperspectral image, we could collect spectral information consisting of thousands of pixels in a short time. Using hyperspectral images, in this work, we developed a label-free technique to detect nanoparticles in different regions of the cell. This technique is based on plasmonic shifts taking place during the interaction of nanoparticles with the surrounding medium. The unique optical properties of gold nanoparticles, localized surface plasmon resonance bands, are influenced by their microenvironment. The LSPR properties of nanoparticles, hence, could provide information on regions in which nanoparticles are distributed. To examine the potential of this technique for intracellular detection, we used three different types of gold nanoparticles: nanospheres, nanostars and Swarna Bhasma (SB), an Indian Ayurvedic/Sidha medicine, in A549 (human non-small cell lung cancer) and HepG2 (human hepatocellular carcinoma) cells. All three types of particles exhibited broader and longer bands once they were inside cells; however, their plasmonic shifts could change depending on the size and morphology of particles. This technique, along with dark-field images, revealed the uniform distribution of nanospheres in cells and could provide more accurate information on their intracellular microenvironment compared to the other particles. The region-dependent optical responses of nanoparticles in cells highlight the potential application of this technique for subcellular diagnosis when particles with proper size and morphology are chosen to reflect the microenvironment effects properly.

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Section: Introductionmentioning

confidence: 99%

Using intracellular plasmonics to characterize nanomorphology in human cells

2020

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“…The local blood supply is compromised by the resection, meaning that selection and preparation of the tissue for anastomosis are important factors in ensuring the success of the procedure. Reperfusion of tissues following periods of ischaemia may result in damage to the parenchyma and the microcirculation in a condition known as ischemia-reperfusion injury [2]. Choosing the optimum surgical strategy is not always trivial however, particularly in upper GI (oesophageal) surgery, where the native blood supply is more tenuous.…”

Section: Introductionmentioning

confidence: 99%

“…This well-known property has been exploited to measure changes in oxygen saturation in tissues such as the skin [10], retina [11], brain [12] and kidney (during open surgery) [13]. Spectral imaging has also been used to investigate the effects of ischaemia-reperfusion injury [2]. The relative concentrations of oxygenated and deoxygenated haemoglobin can be determined using a simple linear spectral unmixing model [14][15][16], diffusion theory [17], inverse Monte Carlo [18] or a non-model-based approach [19].…”

Section: Introductionmentioning

confidence: 99%

Intraoperative measurement of bowel oxygen saturation using a multispectral imaging laparoscope

Clancy

Arya

Stoyanov

et al. 2015

Biomed. Opt. Express

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Intraoperative monitoring of tissue oxygen saturation (StO2 ) has potentially important applications in procedures such as organ transplantation or colorectal surgery, where successful reperfusion affects the viability and integrity of repaired tissues. In this paper a liquid crystal tuneable filter-based multispectral imaging (MSI) laparoscope is described. Motion-induced image misalignments are reduced, using feature-based registration, before regression of the tissue reflectance spectra to calculate relative quantities of oxy- and deoxyhaemoglobin. The laparoscope was validated in vivo, during porcine abdominal surgery, by making parallel MSI and blood gas measurements of the small bowel vasculature. Ischaemic conditions were induced by local occlusion of the mesenteric arcade and monitored using the system. The MSI laparoscope was capable of measuring StO2 over a wide range (30-100%) with a temporal error of ± 7.5%. The imager showed sensitivity to spatial changes in StO2 during dynamic local occlusions, as well as tracking the recovery of tissues post-occlusion.

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“…However, there are several systems reported in the literature that have shown the feasibility of measuring changes in oxygen saturation in tissues such as the skin 9 , retina 10 , brain 11 and kidney 12 . Spectral imaging has also been used to investigate the effects of cold and warm ischaemia-reperfusion injury 13 .…”

Section: Optical Imaging Of Perfusion and Ischaemiamentioning

confidence: 99%

Multispectral imaging of organ viability during uterine transplantation surgery

et al. 2014

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Uterine transplantation surgery has been proposed as a treatment for permanent absolute uterine factor infertility (AUFI) in the case of loss of the uterus. Due to the complexity of the vasculature correct reanastomosis of the blood supply during transplantation surgery is a crucial step to ensure reperfusion and viability of the organ.While techniques such as fluorescent dye imaging have been proposed to visualise perfusion there is no gold standard for intraoperative visualisation of tissue oxygenation. In this paper results from a liquid crystal tuneable filter (LCTF)-based multispectral imaging (MSI) laparoscope are described. The system was used to monitor uterine oxygen saturation (SaO 2 ) before and after transplantation. Results from surgeries on two animal models (rabbits and sheep) are presented.A feature-based registration algorithm was used to correct for misalignment induced by breathing or peristalsis in the tissues of interest prior to analysis. An absorption spectrum was calculated at each spatial pixel location using reflectance data from a reference standard, and the relative contributions from oxy-and deoxyhaemoglobin were calculated using a least squares regression algorithm with non-negativity constraints.Results acquired during animal surgeries show that cornual oxygenation changes are consistent with those observed in point measurements taken using a pulse oximeter, showing reduced SaO 2 following reanastomosis. Values obtained using the MSI laparoscope were lower than those taken with the pulse oximeter, which may be due to the latter's use of the pulsatile arterial blood signal. Future work incorporating immunological test results will help to correlate SaO 2 levels with surgical outcomes.

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Evidence of a heterogeneous tissue oxygenation: renal ischemia/reperfusion injury in a large animal model

Cited by 23 publications

References 24 publications

Using intracellular plasmonics to characterize nanomorphology in human cells

Using intracellular plasmonics to characterize nanomorphology in human cells

Intraoperative measurement of bowel oxygen saturation using a multispectral imaging laparoscope

Multispectral imaging of organ viability during uterine transplantation surgery

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