Evidence of an affinity threshold for IgE‐allergen binding in the percutaneous skin test reaction

Pierson-Mullany, Lisa K.; Jackola, Duaine R.; Blumenthal, Malcolm N.; Rosenberg, Andreas

doi:10.1046/j.0022-0477.2001.01244.x

Cited by 41 publications

(31 citation statements)

References 15 publications

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“…As previously shown [16, 17], interactions between IgE and purified allergens are complex, heterogeneous reactions. The total binding capacity is defined as: CAP ≡ [IgE] × Σ n i = 1 K i × p[K] i , where [IgE] is the specific IgE concentration, n is the number of specific reactions, K i is the equilibrium binding constant for the ith reaction and p[K] i is the relative proportionate amount of each reaction.…”

Section: Resultsmentioning

confidence: 98%

“…On average, those with SPT-positive results have higher reaction binding affinities compared to those with SPT-negative results [17]. Independent investigators have shown that binding affinity is positively correlated with histamine release by basophils in vitro [24].…”

Section: Resultsmentioning

confidence: 99%

“…Our basic experimental method provides information regarding allergen-antibody binding reactions in vitro. Specific details are described elsewhere [16, 17, 21]. The information derived from this method includes the number of reactions involved between an allergen-specific immunoglobulin and the allergen (n), the equilibrium binding affinity constant for each reaction (K A ) and the relative proportion of each reaction to the sum of all reactions involved [p(K)].…”

Section: Methodsmentioning

confidence: 99%

“…Using ultrasensitive chemiluminescence-based detection methods [15], we have devised techniques to study the complex reactions between allergens and IgE [16]. These methods demonstrate that there are clear differences among those who have detectable amounts of serum allergen-specific IgE but who vary with regard to standard SPT sensitization tests; those with SPT-positive results have higher allergen-IgE binding affinity values compared to those who are SPT negative [17]. Low IgE binding affinity failed to elicit an SPT reaction, a clear potential source of classification errors.…”

Section: Introductionmentioning

confidence: 99%

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Allergen Skin Test Reaction Patterns in Children (≤10 Years Old) from Atopic Families Suggest Age-Dependent Changes in Allergen-IgE Binding in Early Life

Jackola

Pierson-Mullany

Blumenthal

et al. 2003

Int Arch Allergy Immunol

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Background: Genetic studies of atopy rely upon evidence of abnormal IgE production, usually elevated total IgE or skin prick test (SPT) reactions. However, these measures may change with subject age. Methods: We screened 1,099 members of atopic families (aged 6–87 years) by serum total IgE and SPT for 14 allergens. For those SPT negative, we screened for Amb a 1- and Der p 1-specific IgE. Der p 1 IgE-Der p 1 allergen binding affinities were done on randomly selected subjects. Results: There were significantly fewer atopics ≤10 years old (69.1%) compared to those >10 years old (75.8%) based upon any SPT-positive result. Children ≤10 years had fewer SPT-positive reactions and smaller SPT wheal reaction areas. Yet, mean total IgE values were comparable to those of the older group. Screens for specific IgE showed no differences in proportions of atopics (≤10 years old = 83.1% and >10 years old = 82.3%). Among those SPT-positive for house dust mite extract, there was a positive correlation between Der p 1 binding affinity and the wheal area of the house dust mite extract. There was a positive correlation between the number of SPT-positive reactions and total IgE for both age groups. However, there was only a significant relationship between SPT-positive wheal area and total IgE for those >10 years old and no apparent relationship between wheal area and total IgE for those ≤10 years old. Conclusion: These results suggest that atopy-specific physiological mechanisms, primarily those involving allergen-IgE binding, change during the earliest years of life.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Allergen Skin Test Reaction Patterns in Children (≤10 Years Old) from Atopic Families Suggest Age-Dependent Changes in Allergen-IgE Binding in Early Life

Jackola

Pierson-Mullany

Blumenthal

et al. 2003

Int Arch Allergy Immunol

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“…From this perspective it is not surprising that monoglycosylated bromelain did not give positive skin tests in patients with IgE antibodies recognizing the glycan moiety of this glycoprotein [15]. Then, the affinity of the interaction between allergen and IgE also plays a role [70, 71]. High-affinity interactions seem to be needed for the triggering of effector cells.…”

Section: Clinical Relevance Of Ige Antibodies Against N-glycansmentioning

confidence: 99%

Carbohydrate Epitopes and Their Relevance for the Diagnosis and Treatment of Allergic Diseases

Ree¹

2002

Int Arch Allergy Immunol

175

119

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Allergenicity of plant and invertebrate N-glycans has been shown to be caused by the presence of two typical nonmammalian substitutions: an α(1,3)-fucose linked to the proximal N-acetylglucosamine and a β(1,2)-xylose linked to the core mannose. IgE antibodies against these carbohydrate structures are induced upon exposure to pollen or after insect stings, and result in extensive cross-reactivity to plant and invertebrate foods. These cross-reactive IgE antibodies have been shown to possess variable degrees of biological activity, but have never been convincingly shown to induce clinical food allergy. The most likely explanation for this lack of clinical relevance has to be sought in a combination of epitope valency and antibody affinity. In diagnostic tests, these antibodies are at the basis of many false-positive test results for food allergy. Recombinant technologies offer the possibility to produce allergens that do not carry IgE-binding glycans. Whether their absence or presence is of importance for the application of recombinant allergens in immunotherapy is still largely unknown.

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The Role of Common Properties in Determining Plant Food Protein Allergenicity

Mills¹,

Jenkins²,

Shewry³

2003

Plant Food Allergens

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Evidence of an affinity threshold for IgE‐allergen binding in the percutaneous skin test reaction

Cited by 41 publications

References 15 publications

Allergen Skin Test Reaction Patterns in Children (≤10 Years Old) from Atopic Families Suggest Age-Dependent Changes in Allergen-IgE Binding in Early Life

Allergen Skin Test Reaction Patterns in Children (≤10 Years Old) from Atopic Families Suggest Age-Dependent Changes in Allergen-IgE Binding in Early Life

Carbohydrate Epitopes and Their Relevance for the Diagnosis and Treatment of Allergic Diseases

The Role of Common Properties in Determining Plant Food Protein Allergenicity

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